Series: Methods in Microbiology

Methods in Microbiology is the most prestigious series devoted to techniques and methodology in the field. Established for over 30 years, Methods in Microbiology provides ready-to-use "recipes", and the latest emerging techniques as well as novel approaches to the tried and tested, established methods.

About the Editors: Colin Harwood, Newcastle University, Newcastle upon Tyne, UK.

Impact Factor 2014: 0.077 (Copyright ISI Journal Citation Report)

To view individual chapters for Methods in Microbiology, please visit Science Direct.

Praise for the Series: "An invaluable resource for the detailed analysis of cellular gene function and genome architecture." - NATURE

Book Series: Current and Emerging Technologies for the Diagnosis of Microbial Infections

Most recent volume


Volume 42. Current and Emerging Technologies for the Diagnosis of Microbial Infections

Published: 18th November 2015 Serial Volume Editors: Andrew Sails Yi Wei Tang
  • Preface
  • Chapter 1: Total Laboratory Automation in Clinical Bacteriology
    • Abstract
    • 1 Clinical Bacteriology and Automation: background
    • 2 Specimen Collection: liquid Microbiology
    • 3 Pre-analytical Automation
    • 4 Pre-analytical Bacteriology Specimen Plating Instruments
    • 5 Digital Plate Reading
    • 6 TLA Systems
    • 7 Change Management: A Holistic Approach to Automation in Bacteriology
  • Chapter 2: MALDI-TOF Mass Spectrometry for Microorganism Identification
    • Abstract
    • 1 Introduction
    • 2 Microbial Identification by MALDI-TOF MS
    • 3 Performance of MALDI-TOF MS for the Identification of Routine Clinical Isolates
    • 4 Direct Identification from Clinical Specimens
    • Limitations and Conclusions
  • Chapter 3: POC Tests in Microbial Diagnostics: Current Status
    • Abstract
    • 1 Introduction
    • 2 The Current Utilisation of Infectious Diseases POC Testing
    • 3 The Road to POC Molecular Diagnostics Is Paved with Good Intentions and Technologies
    • Conclusions
    • Acknowledgements
  • Chapter 4: Molecular Diagnostics in the Diagnosis of Parasitic Infection
    • Abstract
    • 1 Introduction
    • 2 Blood Parasites
    • 3 Tissue Parasites
    • 4 Intestinal Protozoa and Microsporidia
    • 5 Intestinal Helminths
    • 6 Trichomonas Vaginalis
    • 7 Summary
  • Chapter 5: Clinical Applications of Quantitative Real-Time PCR in Virology
    • Abstract
    • 1 Introduction
    • 2 Applications in the Continuum of Care
    • 3 Expanding Applications
    • 4 Discussion
  • Chapter 6: Low-Density TaqMan® Array Cards for the Detection of Pathogens
    • Abstract
    • 1 Introduction
    • 2 TaqMan® Array Cards
    • 3 Developing a TAC Based on In-House Real-Time PCR Assays
    • 4 Syndromic TAC
    • Conclusions
    • Acknowledgements
  • Chapter 7: Invasive Fungal Infections and Approaches to Their Diagnosis
    • Abstract
    • 1 Introduction
    • 2 Spectrum of Opportunistic Pathogens
    • 3 Clinical Recognition of Fungal Infection
    • 4 Laboratory Diagnosis
    • 5 Antifungal Susceptibility Testing
    • Summary and Conclusions
  • Chapter 8: Technical and Software Advances in Bacterial Pathogen Typing
    • Abstract
    • 1 Introduction
    • 2 Pulsed-Field Gel Electrophoresis
    • 3 Serotyping for Salmonella
    • 4 Sequencing-Based Molecular Typing
    • 5 Comparative Genomic Fingerprinting
    • 6 Tools for Pathogen Surveillance and Reporting
    • 7 Geographic Informations Systems
    • 8 Whole-Genome Sequencing
    • 9 Discussion
    • Acknowledgements
  • Chapter 9: Molecular Strain Typing and Characterisation of Toxigenic Clostridium difficile
    • Abstract
    • 1 Introduction
    • 2 Methods for Molecular Strain Typing
    • 3 Comparison of Molecular Typing Methods
    • 4 Impact and Applications of Molecular Strain Typing
    • 5 Standardisation Efforts
    • 6 Future Directions
    • Conclusion
  • Chapter 10: Accurate Whole-Genome Sequencing-Based Epidemiological Surveillance of Mycobacterium Tuberculosis
    • Abstract
    • 1 Introduction
    • 2 Classical Molecular Typing for Molecular-Guided Investigation
    • 3 Whole-Genome-Based Typing for Molecular-Guided Investigation
    • Concluding Remarks
    • Acknowledgements
  • Chapter 11: Solid and Suspension Microarrays for Microbial Diagnostics
    • Abstract
    • 1 Introduction
    • 2 Solid-State Microarrays
    • 3 Suspension Bead Arrays
    • 4 Clinical Applications of Microarray Testing
    • Conclusions
  • Chapter 12: Gene Amplification and Sequencing for Bacterial Identification
    • Abstract
    • 1 Importance of Accurate Identification of Bacteria
    • 2 Evolution of Bacterial Identification Methods
    • 3 16S rRNA Gene Sequencing
    • 4 Sequencing of Other Gene Targets for Bacterial Identification
    • 5 Bacterial Identification by Multilocus and Genome Sequence Analysis
    • 6 Comparison of Gene Sequencing and MALDI-TOF MS for Bacterial Identification
    • 7 Identification by Species-Specific Gene Amplification
    • Conclusion
  • Chapter 13: Host-Based Diagnostics for Detection and Prognosis of Infectious Diseases
    • Abstract
    • 1 Introduction
    • 2 Techniques and Markers
    • 3 Diseases
    • Conclusions
  • Chapter 14: MALDI-TOF Mass Spectrometry in the Clinical Microbiology Laboratory; Beyond Identification
    • Abstract
    • 1 MALDI-TOF MS for Microbial Strain Typing and Epidemiology
    • 2 Resistance Testing by MALDI-TOF MS
    • Concluding Remarks
  • Chapter 15: Next-Generation Sequencing for Pathogen Detection and Identification
    • Abstract
    • 1 Introduction to Next-Generation Sequencing
    • 2 Metagenomic Sequencing
    • 3 NGS for Research Diagnostic Applications
    • 4 Challenges Going Forth and Successful Implementation
  • Chapter 16: Virology: The Next Generation from Digital PCR to Single Virion Genomics
    • Abstract
    • 1 Introduction
    • 2 Digital PCR
    • 3 Viral Load Testing
    • 4 Single Virion Genomics
    • Conclusions
  • Chapter 17: Artificial Nucleic Acid Probes and Their Applications in Clinical Microbiology
    • Abstract
    • 1 Introduction
    • 2 Artificial Nucleic Acids: The Unmet Need Drives Innovation
    • 3 The Many Flavours of Artificial Nucleic Acids; Different Structures, Internal Modifications, and Characteristics
    • 4 Artificial NAs in Use: Versatile, Flexible, and Outperforming DNA/RNA
    • 5 Artificial Probes and Diagnostic Clinical Microbiology
    • Conclusion
  • Additional volumes


    Volume 41. New Approaches to Prokaryotic Systematics

    Published: 25th November 2014 Editors: Michael Goodfellow Iain Sutcliffe Jongsik Chun

    Volume 40. Microbial Synthetic Biology

    Published: 25th November 2013 Serial Volume Editors: Colin Harwood Anil Wipat

    Volume 39. Systems Biology of Bacteria

    Published: 28th November 2012 Serial Volume Editors: Anil Wipat Colin Harwood

    Volume 38. Taxonomy of Prokaryotes

    Published: 13th December 2011 Series Volume Editors: Fred Rainey Aharon Oren

    Volume 37. Immunology of Infection

    Published: 23rd September 2010 Series Volume Editors: Stefan Kaufmann Dieter Kabelitz

    Volume 36. Yeast Gene Analysis

    Published: 25th May 2007 Serial Volume Editor: Ian Stansfield Series Volume Editor: Mike Stark

    Volume 35. Extremophiles

    Published: 7th June 2006 Series Volume Editors: Fred Rainey Aharon Oren

    Volume 34. Microbial Imaging

    Published: 18th December 2004 Serial Volume Editor: Tor Savidge Series Volume Editor: Charalabos Pothulakis

    Volume 33. Functional Microbial Genomics

    Published: 12th December 2002 Series Volume Editors: Brendan Wren Nick Dorrell

    Volume 32. Immunology of Infection

    Published: 25th November 2002 Series Volume Editors: Stefan Kaufmann Dieter Kabelitz

    Volume 31. Molecular Cellular Microbiology

    Published: 30th January 2002 Serial Volume Editors: Philippe Sansonetti Arturo Zychlinsky

    Volume 30. Marine Microbiology

    Published: 2nd May 2001 Serial Volume Editor: John Paul

    Volume 29. Genetic Methods for Diverse Prokaryotes

    Published: 28th May 1999 Serial Volume Editors: Margaret Smith Elizabeth Sockett

    Volume 28. Automation: Genomic and Functional Analyses

    Published: 8th December 1998 Serial Volume Editors: Alister Craig Jörg Hoheisel

    Volume 27. Bacterial Pathogenesis

    Published: 1st July 1998 Serial Volume Editors: Peter Williams Julian Ketley George Salmond

    Volume 25. Immunology of Infection

    Published: 20th April 1998 Serial Volume Editors: Stefan Kaufmann Dieter Kabelitz

    Volume 26. Yeast Gene Analysis

    Published: 15th April 1998 Serial Volume Editor: Mick Tuite

    Volume 24. Techniques for the Study of Mycorrhiza, Part II

    Published: 10th January 1992 Serial Volume Editors: J. Norris David Read A. Varma

    Volume 23. Techniques for the Study of Mycorrhiza

    Published: 14th November 1991 Serial Volume Editors: J. Norris David Read A. Varma

    Volume 22. Methods in Microbiology

    Published: 28th January 1990 Series Volume Editor: Author Unknown

    Volume 20. Electron Microscopy in Microbiology

    Published: 28th January 1988 Serial Volume Editor: Frank Mayer

    Volume 21. Methods in Microbiology

    Published: 28th January 1988 Serial Editors: J. Grinsted P.M. Bennett

    Volume 19. Methods in Microbiology

    Published: 3rd December 1987 Serial Editors: R.R. Colwell R. Grigorova

    Volume 16. Methods in Microbiology

    Published: 28th January 1985 Serial Volume Editor: T. Bergan

    Volume 18. Methods in Microbiology

    Published: 28th January 1985 Serial Volume Editor: Gerhard Gottschalk

    Volume 15. Methods in Microbiology

    Published: 28th January 1984 Serial Editor: T. Bergan

    Volume 17. Methods in Microbiology

    Published: 28th January 1984 Serial Editors: P.M. Bennett J. Grinsted

    Volume 14. Methods in Microbiology

    Published: 28th January 1984 Serial Volume Editor: T. Bergan

    Volume 13. Methods in Microbiology

    Published: 18th December 1979 Serial Volume Editors: T. Bergan J. Norris

    Volume 12. Methods in Microbiology

    Published: 28th January 1979 Serial Volume Editors: T. Bergan J. Norris

    Volume 11. Methods in Microbiology

    Published: 14th December 1978 Serial Volume Editors: T. Bergan J. Norris

    Volume 10. Methods in Microbiology

    Published: 3rd November 1978 Series Volume Editor: Author Unknown

    Volume 9. Methods in Microbiology

    Published: 24th November 1976 Serial Editor: J. Norris

    Volume 8. Methods in Microbiology

    Published: 10th October 1973 Serial Editors: J.R. Norris D.W. Ribbons

    Volume 7B. Methods in Microbiology

    Published: 29th December 1972 Serial Editors: J.R. Norris D.W. Ribbons

    Volume 7A. Methods in Microbiology

    Published: 9th June 1972 Serial Editors: J.R. Norris D.W. Ribbons

    Volume 6B. Methods in Microbiology

    Published: 1st January 1972 Serial Editors: J.R. Norris D.W. Ribbons

    Volume 6A. Methods in Microbiology

    Published: 31st December 1971 Serial Editors: J.R. Norris D.W. Ribbons

    Volume 5B. Methods in Microbiology

    Published: 29th July 1971 Serial Editors: J.R. Norris D.W. Ribbons

    Volume 4. Methods in Microbiology

    Published: 20th April 1971 Series Volume Editor: C. Booth

    Volume 5A. Methods in Microbiology

    Published: 28th January 1971 Serial Editors: J.R. Norris D.W. Ribbons

    Volume 3A. Methods in Microbiology

    Published: 31st March 1970 Serial Editors: J.R. Norris D.W. Ribbons

    Volume 2. Methods in Microbiology

    Published: 28th January 1970 Serial Editors: J.R. Norris D.W. Ribbons

    Volume 1. Methods in Microbiology

    Published: 1st January 1969 Serial Editors: J.R. Norris D.W. Ribbons

    Volume 3B. Methods in Microbiology

    Published: 1st January 1969 Serial Editors: J.R. Norris D.W. Ribbons