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Volume . Plant Molecular Biology LabFax

Published: 2nd December 2012 Series Volume Editor: R. Croy Series Editors: Ali Hames D. Rickwood

Contributors

Abbreviations

1. Plant Anatomy, Histology and Taxonomy for Molecular Biologists

Histology: Plant Cell Types

Parenchyma

Epidermis

Mesophyll

Endodermis

Pericycle

Collenchyma

Sclerenchyma

Vascular bundles

Xylem

Phloem

Cambium

Anatomy

Stem

Root

Leaf

Flower

Fruits and Seeds

Methods for Examination of Plant Tissues

Embedding and Sectioning

Histochemical Staining

Fluorescence Microscopy

General Information

Procedure

Commonly Used Fluorochromes

Taxonomy

Rules for the Citation of Plant Names in Scientific Publications

Nomenclature of Major Crop and Experimental Plants

References

Further Reading

2. Plant Nucleic Acids

DNA Isolation from Plants

Isolation of Agrobacterium Ti Plasmid DNA

Calculation of Gene Copy Number Equivalents

Formula

Nomogram

Computer Program

RNA Isolation from Plants

Codon Usage

References

3. Plant Gene Index

Introduction

References

4. Plant Transformation and Expression Vectors

Introduction

Expression Cassettes

pRT103

pJIT74

pDH51

pJIT60

pMA406

pKYLX3

PJIT117

Reporter Genes

Gus Gene

Cat and Luc Genes

Other Reporter Genes

Selectable Markers

Kanamycin Resistance

Hygromycin Resistance

Bialaphos Resistance

Sulfonamide Resistance

Streptomycin/Spectinomycin Resistance

Chlorsulfuron Resistance

Methotrexate Resistance

Phenotypic Markers

Anthocyanin Regulatory Genes

Binary Vectors

pBIN19

pROK1

pGA482

pCGN1547, pCGN1548

pUCD2340

pC22

References

5. Plant Selectable Genes, Reporter Genes and Promoters

Introduction

Aminoglycoside Adenyltransferase Gene [S]

Bleomycin-Resistance Gene [S]

Cauliflower Mosaic Virus 35S Promoter [P]

Cauliflower Mosaic Virus 35S Promoter and Terminator [P,T]

Chloramphenicol Acetyltransferase Gene [R]

Dihydrofolate Reductase [S]

Dihydropteroate Synthase Gene [S]

Enolpyruvylshikimate Phosphate Synthase Gene [S]

β-Galactosidase Gene [RH]

β-Glucuronidase Gene [RH]

Hygromycin Phosphotransferase Gene [S]

Luciferase Gene [R]

Luciferase A and B Genes [R]

Neomycin Phosphotransferase Gene [S, R]

Nopaline Synthase Gene [R, P, T]

Octopine Synthase Gene [R, P, T]

Phosphinothricin Acetyltransferase Gene [S]

References

6. Characteristics of Plant Pre-mRNA Introns and Transposable Elements

Introduction

Mobile Genetic Elements in Higher Plants

References

7. Plant Tissue Culture and Genetic Transformation

Tissue Culture Media

Common Basal Media

Media Supplements

Media and Methods for the Culture of Tobacco

Genetic Transformation

Gene Transfer Methods and their Evaluation

Stably Transformed Plant Species

Agrobacterium Strains, Media and Methods

Protoplast Transformation

Particle Bombardment of Tissues

Examples of Tissue-Specific Transient Expression

References

8. Plant Organellar Targeting Sequences

Nucleus

Peroxisomes/Glyoxysomes (Microbodies)

Secretion Pathway

Mitochondria

Chloroplasts

Other Plastids

References

9. Chloroplast Molecular Biology

Introduction

What cpDNA Encodes

Nomenclature of Genes and their Products

Genes for the Polypeptides of Multi-Component Thylakoid Complexes Involved in Electron Transport

Photosystem I (PSI) Plastocyanin: Ferredoxin Oxidoreductase

Photosystem II (PSII) Water: Plastoquinone Oxidoreductase

Cytochrome Blf Complex (Plastoquinol: Plastocyanin Oxidoreductase)

ATP Synthase (F0F1-ATPase, EC 3.6.1.34)

NADPH: Plastoquinone Oxidoreductaste (NADPH Dehydrogenase)

The Chloroplast Genetic System

Ribosomal RNA

Chloroplast Ribosomal Proteins

tRNA Genes

RNA Polymerase

Chloroplast Gene Expression

Transcription

Introns, Splicing and Processing

Chloroplast Genome Structure

Gene Arrangements

Chloroplast Transformation

Transformation of Plastids of Higher Plants

Transformation of the Chloroplast of Chlamydomonas reinhardtii

Markers for Selection of Transformants in C. reinhardtii

Apparatus for Particle Bombardment

References

10. Plant Gene Expression and PCR Techniques

PCR Strategies

Assay of Plant Gene Expression

Introduction

References

Index

Additional volumes


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