Biothiols, Part B: Glutathione and Thioredoxin: Thiols in Signal Transduction and Gene Regulation - 1st Edition - ISBN: 9780121821531, 9780080883656

Biothiols, Part B: Glutathione and Thioredoxin: Thiols in Signal Transduction and Gene Regulation, Volume 252

1st Edition

Editor-in-Chiefs: John Abelson Melvin Simon
Serial Volume Editors: Helmut Sies
eBook ISBN: 9780080883656
Imprint: Academic Press
Published Date: 27th July 1995
Page Count: 382


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Table of Contents

Glutathione: Distribution, Biosynthesis, Metabolism, and Transport:

D.P. Jones, Glutathione Distribution in Natural Products: Absorption and Tissue Distribution.

L.H. Lash, Intracellular Distribution of Thiols and Disulfides: Assay of Mitochondrial Glutathione Transport.

A. Meister, Glutathione Biosynthesis and Its Inhibition.

W.W. Wells, D.P. Xu, and M.P. Washburn, Glutathione: Dehydroascorbate Oxidoreductases.

F. Ursini, M. Maiorino, R. Brigelius-Flohé, K.D. Aumann, A. Roveri, D. Schomburg, and L. Flohé, Diversity of Glutathione Peroxidases.

D.J. Meyer and B. Ketterer, Purification of Soluble Human Glutathione S-Transferases.

H.J. Forman, M.M. Shi, T. Iwamoto, R.-M. Liu, and T.W. Robinson, Measurement of g-Glutamyl Transpeptidase and g-Glutamylcysteine Synthetase Activities in Cells.

T. Kondo, G.L. Dale, and E. Beutler, Thiol Transport from Human Red Blood Cells.

J.A. Fuchs, Glutathione Mutants.

D.J. Reed, Cystathionine.

T. Meier and R.D. Issels, Promotion of Cyst(e)ine Uptake.

Signal Transduction and Gene Regulation:

T.P. Holler and P.B. Hopkins, Ovothiols.

S.R. Hubbard, X-Ray Absorption Spectroscopy Studies of Protein Kinase C.

R. Gopalakrishna, Z.H. Chen, and U. Gundimeda, Modificiations of Cysteine-Rich Regions in Protein Kinase C Induced by Oxidant Tumor Promoters and Enzyme-Specific Inhibitors.

F.J. Romero and J. Romá, Glutathione and Protein Kinase C in Peripheral Nervous Tissues.

A.F.G. Quest, E.S.G. Bardes, W.Q. Xie, E. Willott, R.A. Borchardt, and R.M. Bell, Expression of Protein Kinase Cg Regulatory Domain Elements Containing Cysteine-Rich Zinc-Coordinating Regions as Glutathione S-Transferase Fusion Proteins.

F.J.T. Staal, M.T. Anderson, and L.A. Herzenberg, Redox Regulation of Activation of NF-kB Transcription Factor Complex: Effects of N-Acetycysteine.

Y.J. Suzuki and L. Packer, Redox Regulation of DNA-Protein Interaction by Biothiols.

M. Mizuno and L. Packer, Suppression of Protooncogene C-fos Expression by Antioxidant Dihydrolipoic Acid.

M.S. Patel, N.N. Vettakkoruma-Kankav, and T.-C. Liu, Dihydrolipoamide Dehydrogenase: Activity Assays.

Thioredoxin and Glutaredoxin:

A. Holmgren and M. Björnstedt, S. Kumar, and A. Holmgren, Selenite and Selenodiglutathione: Reactions with Thioredoxin Systems.

R.S. Hutchison and D.R. Ort, Measurement of Equilibrium Midpoint Potentials of Thiol/Disulfide Regulatory Groups on Thioredoxin-Activated Chloroplast Enzymes.

J.H. Wong, K. Kobrehel, and B.B. Buchanan, Thioredoxin and Seed Proteins.

J.-P. Jacquot, E. Issakidis, P. Decottignies, M. Lemaire, and M.-M. Myroslawa, Analysis and Manipulation of Target Enzymes for Thioredoxin Control.

K. Schulze-Osthoff, H. Schenk, and W. Dröge, Effects of Thioredoxin on Activation Transcription Factor NF-KB.

M. Russel, Thioredoxin Genetics.

P. Schürmann, Ferredoxin: Thioredoxin System.

A. Holmgren and F. Åslund, Flutaredoxin.

H.J. Dyson, Nuclear Magnetic Resonance of Thioredoxin and Glutaredoxin.

Mimics of Biological Thiols and Thiol Inhibitors:

R.S. Glass and T.C. Stadtman, Selenophosphate.

M.-O. Christen, Anethole Dithiolethione: Biochemical Considerations.

M.-O. Christen, L. Fackir, and D. Jore, Use of Linoleic Acid Radiolysis for Assay of Antioxidant Action of Anethole Dithiolethione.

P. Rat, M.-O. Christen, M. Thevenin, J.-M. Warnet, and M. Adolphe, Cold Light Fluorimetry: A Microtritration Technology for Cell Culture to Evaluate Anethole dithiolethione and Other Biothiols.

H. Sies, Ebselen.

N. Sato, S. Iwata, A. Yamauchi, T. Hori, and J. Yodoi, Thiol Compounds and Adult T-Cell Leukemia Virus Infection: A Potential Therapeutic Approach. Author Index. Subject Index.


The critically acclaimed laboratory standard for forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerlyawaited, frequently consulted, and praised by researchers and reviewers alike. More than 250 volumes have been published (all of them still in print) and much of the material is relevant even today--truly an essential publication for researchers in all fields of life sciences.

Key Features

@introbul:Key Features @bul:* Distribution, biosynthesis, metabolism, and transport of glutathione

  • Signal transduction and gene regulation
  • Thioredoxin and glutaredoxin
  • Mimics of biological thiols and thiol inhibitors


Biochemists, molecular biologists, physiologists, cell biologists, and biomedical researchers.


No. of pages:
© Academic Press 1995
Academic Press
eBook ISBN:


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About the Editor-in-Chiefs

John Abelson Editor-in-Chief

Affiliations and Expertise

California Institute of Technology, Division of Biology, Pasadena, U.S.A.

Melvin Simon Editor-in-Chief

Affiliations and Expertise

The Salk Institute, La Jolla, CA, USA

About the Serial Volume Editors

Helmut Sies Serial Volume Editor

Helmut Sies is an Honorary Member of the American Society for Biochemistry and Molecular Biology. He received an Honorary Ph.D. from the University of Buenos Aires, Argentina in 1996. Dr. Sies is a member of the Northrhine-Westphalian Academy of Sciences, Germany, and a Corresponding Member of both the Academy of Sciences of Heidelberg, Germany, and the Academy of Medicine, Buenos Aires, Argentina. He has received many awards and prizes, including the FEBS Anniversary Prize awarded by the Federation of European Biochemical Societies, 1978; the Distinguished Foreign Scholar award, MASUA, 1985; the Silver Medal, Karolinska Institute, Stockholm, 1986; the Ernst Jung Preis fur Medizin, 1988; the Claudius-Galenus-Preis, 1990; and the ISFE-Preis, 1994. Dr. Sies sereves on the editorial board and advisory committee for twelve journals, has edited numerous books, and has published more than 400 original articles and chapters. He received his M.D. at the University of Munich in 1967 and currently serves as Full Professor and Chairman of the Department of Physiological Chemistry at the University of Düsseldorf.

Affiliations and Expertise

Institut Fur Physiologische Chemie, Germany