The critically acclaimed laboratory standard for forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerlyawaited, frequently consulted, and praised by researchers and reviewers alike. More than 250 volumes have been published (all of them still in print) and much of the material is relevant even today--truly an essential publication for researchers in all fields of life sciences.

Key Features

@introbul:Key Features @bul:* Distribution, biosynthesis, metabolism, and transport of glutathione * Signal transduction and gene regulation * Thioredoxin and glutaredoxin * Mimics of biological thiols and thiol inhibitors


Biochemists, molecular biologists, physiologists, cell biologists, and biomedical researchers.

Table of Contents

Glutathione: Distribution, Biosynthesis, Metabolism, and Transport: D.P. Jones, Glutathione Distribution in Natural Products: Absorption and Tissue Distribution. L.H. Lash, Intracellular Distribution of Thiols and Disulfides: Assay of Mitochondrial Glutathione Transport. A. Meister, Glutathione Biosynthesis and Its Inhibition. W.W. Wells, D.P. Xu, and M.P. Washburn, Glutathione: Dehydroascorbate Oxidoreductases. F. Ursini, M. Maiorino, R. Brigelius-Flohé, K.D. Aumann, A. Roveri, D. Schomburg, and L. Flohé, Diversity of Glutathione Peroxidases. D.J. Meyer and B. Ketterer, Purification of Soluble Human Glutathione S-Transferases. H.J. Forman, M.M. Shi, T. Iwamoto, R.-M. Liu, and T.W. Robinson, Measurement of g-Glutamyl Transpeptidase and g-Glutamylcysteine Synthetase Activities in Cells. T. Kondo, G.L. Dale, and E. Beutler, Thiol Transport from Human Red Blood Cells. J.A. Fuchs, Glutathione Mutants. D.J. Reed, Cystathionine. T. Meier and R.D. Issels, Promotion of Cyst(e)ine Uptake. Signal Transduction and Gene Regulation: T.P. Holler and P.B. Hopkins, Ovothiols. S.R. Hubbard, X-Ray Absorption Spectroscopy Studies of Protein Kinase C. R. Gopalakrishna, Z.H. Chen, and U. Gundimeda, Modificiations of Cysteine-Rich Regions in Protein Kinase C Induced by Oxidant Tumor Promoters and Enzyme-Specific Inhibitors. F.J. Romero and J. Romá, Glutathione and Protein Kinase C in Peripheral Nervous Tissues. A.F.G. Quest, E.S.G. Bardes, W.Q. Xie, E. Willott, R.A. Borchardt, and R.M. Bell, Expression of Protein Kinase C


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© 1995
Academic Press
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About the serial-volume-editor

Helmut Sies

Helmut Sies is an Honorary Member of the American Society for Biochemistry and Molecular Biology. He received an Honorary Ph.D. from the University of Buenos Aires, Argentina in 1996. Dr. Sies is a member of the Northrhine-Westphalian Academy of Sciences, Germany, and a Corresponding Member of both the Academy of Sciences of Heidelberg, Germany, and the Academy of Medicine, Buenos Aires, Argentina. He has received many awards and prizes, including the FEBS Anniversary Prize awarded by the Federation of European Biochemical Societies, 1978; the Distinguished Foreign Scholar award, MASUA, 1985; the Silver Medal, Karolinska Institute, Stockholm, 1986; the Ernst Jung Preis fur Medizin, 1988; the Claudius-Galenus-Preis, 1990; and the ISFE-Preis, 1994. Dr. Sies sereves on the editorial board and advisory committee for twelve journals, has edited numerous books, and has published more than 400 original articles and chapters. He received his M.D. at the University of Munich in 1967 and currently serves as Full Professor and Chairman of the Department of Physiological Chemistry at the University of Düsseldorf.


@from:Praise for the Series @qu:"The Methods in Enzymology series represents the gold-standard." @source:--NEUROSCIENCE @qu:"Incomparably useful." @source:--ANALYTICAL BIOCHEMISTRY @qu:"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." @source:--BIO/TECHNOLOGY @qu:"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." @source:--CHEMISTRY IN INDUSTRY @qu:"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." @source:--AMERICAN SOCIETY OF MICROBIOLOGY NEWS @qu:"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work." @source:--ENZYMOLOGIA @qu:"A series that has established itself as a definitive reference for biochemists." @source:--JOURNAL OF CHROMATOGRAPHY