Description

The critically acclaimed laboratory standard for forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerlyawaited, frequently consulted, and praised by researchers and reviewers alike. More than 250 volumes have been published (all of them still in print) and much of the material is relevant even today--truly an essential publication for researchers in all fields of life sciences.

Key Features

@introbul:Key Features @bul:* Prevention of oxidative stress * Redox regulation of metabolism * Toxicology * Carcinogenesis

Readership

Biochemists, molecular biologists, physiologists, cell biologists, and biomedical researchers.

Table of Contents

Overview: A. Meister, Glutathione Metabolism. H.F. Gilbert, Thiol/Disulfide Exchange Equilibria and Disulfide Bond Stability. Thiyl Radicals: P. Wardman and C. von Sonntag, Kinetic Factors That Control the Fate of Thiyl Radicals in Cells. C. Schineich, Kinetics of Thiol Reactions. S.A. Everett and P. Wardman, Perthiols as Antioxidants: Radical-Scavenging and Pro-oxidative Mechanisms. S.C. Kundu and R.L. Willson, Thiyl (Sulfhydryl/Thiol) Free Radical Reactions, Vitamins, Beta-Carotene, and Superoxide Dismutase in Oxidative Stress: Design and Interpretation of Enzymatic Studies. C.C. Winterbourn and D. Metodiewa, Reaction of Superoxide with Glutathione and Other Thiols. L.M. Weiner, Quantitative Determination of Thiol Groups in Low and High Molecular Weight Compounds by Electron Paramagnetic Resonance. E. Cadenas, Thiyl Radical Formation during Thiol Oxidation by Ferrylmyoglobin. R. Munday, In Vivo Toxicity of Thiols: Relationship to Rate of One-Electron Oxidation by Oxyhemoglobin. Chemical Basis of Thiol/Disulfide Measurements: N.S. Kosower and E.M. Kosower, Diamide: An Oxidant Probe for Thiols. E.M. Kosower and N.S. Kosower, Bromobimane Probes for Thiols. G.L. Newton and R.C. Fahey, Determination of Biothiols by Bromobimane Labeling and High-Performance Liquid Chromatography. R. Singh, G.V. Lamoureux, W.J. Lees, and G.M. Whitesides, Reagents for Rapid Reduction of Disulfide Bonds. K. Becker and R.H. Schirmer, 1,3-Bis(2-chloroethyl)-1-nitrosourea as Thiol-Carbamoylating Agent in Biological Systems. Monothiols: Measurement in Organs, Cells, Organel

Details

No. of pages:
529
Language:
English
Copyright:
© 1995
Published:
Imprint:
Academic Press
Print ISBN:
9780121821524
Electronic ISBN:
9780080883649

Reviews

@from:Praise for the Series @qu:"The Methods in Enzymology series represents the gold-standard." @source:--NEUROSCIENCE @qu:"Incomparably useful." @source:--ANALYTICAL BIOCHEMISTRY @qu:"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." @source:--BIO/TECHNOLOGY @qu:"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." @source:--CHEMISTRY IN INDUSTRY @qu:"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." @source:--AMERICAN SOCIETY OF MICROBIOLOGY NEWS @qu:"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work." @source:--ENZYMOLOGIA @qu:"A series that has established itself as a definitive reference for biochemists." @source:--JOURNAL OF CHROMATOGRAPHY