Description

The use of thermodynamics in biological research can be equated to an energy book-keeping system. While the structure and function of a molecule is important, it is equally important to know what drives the energy force. This volume presents sophisticated methods for estimating the thermodynamic parameters of specific protein-protein, protein-DNA and small molecule interactions.

Key Features

* Elucidates the relationships between structure and energetics and their applications to molecular design, aiding researchers in the design of medically important molecules
* Provides a "must-have" methods volume that keeps MIE buyers and online subscribers up-to-date with the latest research
* Offers step-by-step lab instructions, including necessary equipment, from a global research community

Readership

Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists

Table of Contents

  1. A Thermodynamic Approach for the Targeting of Nucleic Acid Structures Using Their Complementary Single Strands
  2. Hui-Ting Lee, Caroline Carr, Hollie Siebler, Lela Waters, Irine Khutsishvili, Fany Iseka, Brian Domack, Chris M. Olsen, and Luis Marky

  3. Thermodynamics of Biological Processes
  4. Hernan G. Garcia, Jane Kondev, Nigel Orme, Julie Theriot and Rob Phillips

  5. Protein stability in the presence of co-solutes
  6. Luis Marcelo F. Holthauzen, Matthew Auton, Mikhail Sineva and Jorg Rosgen

  7. Small Angle X-ray Scattering Studies of Peptide-Lipid Interactions using the Mouse Paneth Cell α-Defensin Cryptdin-4
  8. Abhijit Mishra, Kenneth P. Tai, Nathan W. Schmidt, André J. Ouellette and Gerard C. L. Wong

  9. Synergy of molecular dynamics and isothermal titration calorimetry in studies of allostery
  10. Rebecca Strawn, Thomas Stockner, Milan Melichercik, Lihua Jin, Wei-Feng Xue, Jannette Carey and Rüdiger Ettrich

  11. Using tryptophan fluorescence to measure the stability of membrane proteins folded in liposomes
  12. C. Preston Moon1 and Karen G. Fleming

  13. Non-B Conformations of CAG Repeats Using 2-aminopurine
  14. Natalya N. Degtyareva and Jeffrey T. Petty

  15. Disulfide bond-mediated passenger domain stalling as a structural probe of autotransporter outer membrane secretion in vivo
  16. Jonathan P. Renn & Patricia L. Clark

     

  17. Strategies for the Thermodynamic Characterization of Linked Binding/Local Folding Reactions Within the Native State: Application to the LID Domain of Adenylate Kinase from Escherichia coli
  18. Travis P. Schrank, W. Austin Elam, Jing Li and Vincent J. Hilser

  19. Fluorescence detected sedimentation in dilute and highly concentrated solutions<

Details

No. of pages:
369
Language:
English
Copyright:
© 2011
Published:
Imprint:
Academic Press
Print ISBN:
9780123860033
Electronic ISBN:
9780123860040