In the past several years, there has been an explosion in the ability of biologists, molecular biologists and biochemists to collect vast amounts of data on their systems. This volume presents sophisticated methods for estimating the thermodynamic parameters of specific protein-protein, protein-DNA and small molecule interactions.

The use of thermodynamics in biological research is used as an “energy book-keeping system.” While the structure and function of a molecule is important, it is equally important to know what drives the energy force. These methods look to answer: What are the sources of energy that drive the function? Which of the pathways are of biological significance?

As the base of macromolecular structures continues to expand through powerful techniques of molecular biology, such as X-ray crystal data and spectroscopy methods, the importance of tested and reliable methods for answering these questions will continue to expand as well.

Key Features

* Elucidates the relationships between structure and energetics and their applications to molecular design, aiding researchers in the design of medically important molecules
* Provides a "must-have" methods volume that keeps MIE buyers and online subscribers up-to-date with the latest research
* Offers step-by-step lab instructions, including necessary equipment, from a global research community


Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists.

Table of Contents

  1. Measurement and Analysis of Equilibrium Binding Titrations: A beginner’s guide
  2. Dorothy Beckett

  3. Macromolecular Competition Titration Method: Accessing Thermodynamics of the Unmodified Macromolecule – Ligand Interactions Through Spectroscopic Titrations of Fluorescent Analogs
  4. Wlodzimierz Bujalowski and Maria J. Jezewska

  5. Analysis of PKR-RNA interactions by sedimentation velocity
  6. C. Jason Wong, Katherine Launer-Felty and James L. Cole

  7. Structural and Thermodynamic Analysis of PDZ/Ligand Interactions
  8. Tyson R. Shepherd and Ernesto J. Fuentes

  9. Thermodynamic analysis of metal ion-induced protein assembly
  10. Andrew B. Herr and Deborah G. Conrady

  11. Thermodynamic Dissection of Colicin Interactions
  12. Nicholas G Housden and Colin Kleanthous

  13. Energetics of Src homology domain interactions in receptor tyrosine kinase-mediated signaling
  14. John E. Ladbury and Stefan T. Arold

  15. Structural and Functional Energetic Linkages in Allosteric Regulation of Muscle Pyruvate Kinase
  16. J. Ching Lee and Petr Herman

  17. Analysis of Free Energy versus Temperature Curves in Protein Folding and Macromolecular Interactions
  18. Vince J. LiCata and Chin-Chi Liu

  19. Application of the Sequential n-Step Kinetic Mechanism to Polypeptide Translocases
  20. Aaron L. Lucius, Justin M. Miller and Burki Rajendar

  21. A Coupled Equilibrium Approach to Study Nucleosome Thermodynamics
  22. Andrew J. Andrews and Karolin Luger

  23. Quantitative methods for measuring DNA flexibility in vitro and in vivo
  24. Justin P. Peters, Nicole Becker, Emily M. Rueter, Zeljko Bajzer, Jason D. Kahna and L. Jame


No. of pages:
© 2011
Academic Press
eBook ISBN:
Print ISBN:

About the serial-volume-editor

Melvin Simon

Affiliations and Expertise

The Salk Institute, La Jolla, CA, USA