Biothermodynamics, Part B

Biothermodynamics, Part B

1st Edition - November 11, 2009

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  • Editors: Michael Johnson, Jo Holt, Gary Ackers
  • eBook ISBN: 9780080887814
  • Hardcover ISBN: 9780123747761

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The use of thermodynamics in biological research can be equated to an energy book-keeping system. While the structure and function of a molecule is important, it is equally important to know what drives the energy force. These methods look to answer: What are the sources of energy that drive the function? Which of the pathways are of biological significance? As the base of macromolecular structures continues to expand through powerful techniques of molecular biology, such as X-ray crystal data and spectroscopy methods, the importance of tested and reliable methods for answering these questions will continue to expand as well. This volume presents sophisticated methods for estimating the thermodynamic parameters of specific protein-protein, protein-DNA and small molecule interactions.

Key Features

  • Elucidates the relationships between structure and energetics and their applications to molecular design, aiding researchers in the design of medically important molecules
  • Provides a "must-have" methods volume that keeps MIE buyers and online subscribers up-to-date with the latest research
  • Offers step-by-step lab instructions, including necessary equipment, from a global research community


Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists

Table of Contents

  • 1. NMR-based approaches for assessing effects of macromolecular crowding on globular proteins
    Gary Pielak, Andrew Miklos, Lisa Charlton and Conggang Li
    2. Fluorescence spectroscopy in thermodynamic and kinetic analysis of membrane protein insertion
    Alexey S. Ladokhin
    3. Evaluating the energy-dependent "binding" in the early stage of protein import into chloroplasts
    Mitsuru Akita and Hitoshi Inoue
    4. DNA length-dependent periodic properties of cooperative protein-DNA complexes
    Michael Fried
    5. Applying thermodynamic linkage to the study of pyruvate kinase
    Aron Fenton
    6. Conformational Stability of Cytochrome C
    Reinhard Schweitzer-Stenner
    7. Metadynamics methods applied to ion channel gating
    Carmen Domene
    8. Measuring Co-translational Conformations of Nascent Polypeptide Chains
    Patricia L. Clark & Krastyu G. Ugrinov
    9. Taking an energetic approach to assess and explain cooperative gating phenomena in voltage-dependent potassium channels
    Yifrach Ofer, Zandany Nitzan and Tzilhav Shem-Ad
    10. Energetics of the collagen triple helix
    Yujia Xu
    11. Use of pressure perturbation calorimetry to characterize the volumetric properties of proteins
    Katrina L. Schweiker and George Makhatadze
    12. Thermodynamic approaches to nucleosome-histone interactions
    Andrew Andrews and Karolin Luger
    13. Thermodynamic approaches to carbohydrate binding by the agrin-G3 domain
    Andrei T. Alexandrescu
    14. Energetic methods applied to allostery in thrombin
    Enrico Di Cera
    15. Thermodynamics of protein translocation
    Arnold J.M Driessen and Alexej Kedrov
    16. Solvent denaturation of proteins and the interpretation of the m-value
    J. Martin Scholtz and Nick Pace
    17. Energetic approaches to the characterization of the E. coli PriA helicase
    Wlodek Bujalowski
    18. Protein-protein interactions and macromolecular stability
    Lisa Gloss
    19. Methods for assessing T cell receptor recognition
    Brian M Baker
    20. Thermodynamic analysis applied to bromodomain-histone interactions
    Martin Thompson
    21. Thermodynamic parameters fo dimer-dimer interaction in 2-cys peroxiredoxin
    Karl-Josef Dietz
    22. Small molecule-membrane interactions
    Anne Hinderliter
    23. Constant pH molecular dynamics simulations
    Jana Khandogin Shen

Product details

  • No. of pages: 676
  • Language: English
  • Copyright: © Academic Press 2009
  • Published: November 11, 2009
  • Imprint: Academic Press
  • eBook ISBN: 9780080887814
  • Hardcover ISBN: 9780123747761

About the Editors

Michael Johnson

Affiliations and Expertise

University of Virginia Health Sciences Center, Charlottesville, USA

Jo Holt

Affiliations and Expertise

Washington University Medical Center, St. Louis, MO, USA

Gary Ackers

Affiliations and Expertise

Washington University School of Medicine, St. Louis, Mo, USA

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