Suggested Schedule for Laboratory Exercises.
Record Keeping and Safety Rules.
Format of Student Laboratory Records.
The Ten Commandments of Record Keeping.
Safety Rules in the Laboratory.
Exercise 1: Aseptic Culture and Establishing Pure Cultures.
Exercise 2: Preparation of Culture Media.
Exercise 3: The Growth Curve.
Exercise 4: Isolation of Plasmid DNA from Escherichia coli: The Mini-Prep.
Exercise 5: Purification, Concentration, and Quantitation of DNA.
Exercise 6: Large-Scale Isolation of Plasmid DNA by Column Chromatography.
Exercise 7: Amplification of a lacZ.
Gene Fragment by the Polymerase Chain Reaction.
Exercise 8: Restriction Digestion and Agarose Gel Electrophoresis.
Exercise 9: Southern Transfer.
Exercise 10: Preparation, Purification, and Hybridization of Probe.
Exercise 11: Transformation of Saccharomyces cerevisiae.
Exercise 12: Isolation of Plasmid from Yeast and Escherichia coli Transformation.
Exercise 13: Protein Assays.
Exercise 14: Qualitative Assay for Galactosidase in Yeast Colonies.
Exercise 15: Determination of Galactosidase in Permeabilized Yeast Cells.
Exercise 16: Assay of Galactosidase in Cell Extracts.
Exercise 17: Galactosidase Purification.
Exercise 18: Western Blot: Probe of Protein Blot with Antibody to Galactosidase.
Appendix 1: Alternate Protocols and Experiments:
Exercise 1A: Isolation and Characterization of Auxotrophic Yeast Mutants.
Exercise 2A: Measurement of pH.
Exercise 3A: Use of the Spectrophotometer.
Exercise 6A: Isolation of Plasmid DNA: The Maxi-Prep.
Exercise 10A: Colony Hybridiz