Biosynthesis, Modification, and Processing of Cellular and Viral Polyproteins

Biosynthesis, Modification, and Processing of Cellular and Viral Polyproteins presents the proceedings of the international conference held in Hamburg-Blankenese on May 27-31, 1980. The book discusses topics on polyproteins, polyfunctional proteins, precursor proteins, as well as the differences in synthesis and processing of ACTH/endorphin peptides in primary cultures of anterior and intermediate lobes of the pituitary. The text also presents evidence for a self-cleaving precursor of virus-coded protease, RNA-replicase, and VPg; and the role of Pr180gag-pol in the characterization and genetic analysis of retrovirus maturation.

Contributors

Foreword

Preface

Opening Remarks

Polyproteins-Polyfunctional Proteins-Precursor Proteins

I. Biosynthesis of Precursors

Neuropolypeptides Illustrate a New Perspective in Mammalian Protein Synthesis—The Composite Common Precursor

A. Hypothalamic Precursors

Biosynthetic Precursors of Neurohormones: High Molecular Weight Immunoreactive Forms of Neurophysin:Vasopressin and of Somatostatin

On the Biosynthetic Origin of Neurophysin-Neurohypophyseal Peptide Hormone Complexes

Hypothalamic mRNA-Directed Synthesis of Neuropolypeptides: Immunological Identification of Precursors to Neurophysin II/Arginine Vasopressin and to Neurophysin I/Oxytocin

Efforts Directed Toward an Understanding of the Biosynthesis of the Hypothalamic-Releasing Hormones

Immunological, Enzymological, and Chemical Characterization of a Large Somatostatin-Like Polypeptide

B. Pituitary Precursors

Pro-Opiomelanocortin: A New Concept Issued from ACTH and LPH Biosynthetic Studies

Biosynthesis of the Enkephalins: Precursors and Intermediates

C. Viral Precursors

Synthesis and Maturation of the Vesicular Stomatitis Virus Glycoprotein

II. Processing and Modifications

Differences in Synthesis and Processing of ACTH/Endorphin Peptides in Primary Cultures of Anterior and Intermediate Lobes of the Pituitary

Post-Translational Processing of Pro-ACTH/Endorphin in Rat Pituitary

The Role of the Carbohydrate in the Stabilization, Processing, and Packaging of Pro-ACTH/αMSH-Endorphin in Frog and Mouse Pituitary Neurointermediate Lobes

Polyadenylylation of Reovirus Proteins

Processing of the Influenza Virus Hemagglutinin

Alterations in the Processing and Shedding of Retrovirus Proteins in Friend Erythroleukemia Cells

Murine Leukemia Virus Polyprotein Processing

Processing and Structure of Murine Leukemia Virus Gag and Env Gene-Encoded Polyproteins

Synthesis and Processing of the Internal Structural Proteins of Retroviruses: Site of Synthesis, Evidence for Multiply Charged Species, and Analysis of a Mutant Defective in Processing

Influence of Medium Hyperosmolanty and of Guanidine on the Synthesis and Processing of Poliovirus Proteins

III. Virus-Coded Proteases

Evidence for a Self-Cleaving Precursor of Virus-Coded Protease, RNA-Replicase, and VPg

Protein Synthesis and Cleavage in Picornavirus-Infected Cells

Polyprotein Processing by Avian Viral Protease

IV. Genetic Analysis

Characterization and Genetic Analysis of Retrovirus Maturation: A Role for Pr180gag-po1

VPg: The Genome-Linked Protein of Picornaviruses

Organization of Human Genomic DNA Encoding Pro-Opiomelanocortin

Concluding Remarks: Post Translational Modifications of Proteins in Discrete Subcellular Organelles

Index