This welcome new edition discusses bioprocess engineering from the perspective of biology students. It includes a great deal of new material and has been extensively revised and expanded. These updates strengthen the book and maintain its position as the book of choice for senior undergraduates and graduates seeking to move from biochemistry/microbiology/molecular biology to bioprocess engineering.

Key Features

New to this edition:

  • All chapters thoroughly revised for current developments, with over 200 pgs of new material, including significant new content in:
    • Metabolic Engineering
    • Sustainable Bioprocessing
    • Membrane Filtration
    • Turbulence and Impeller Design
    • Downstream Processing
    • Oxygen Transfer Systems

  • Over 150 new problems and worked examples
  • More than 100 new illustrations


(a) Senior undergraduate students in applied biology, biomedical engineering, or chemical engineering taking final year options in bioprocessing/biotechnology (b) industrial practitioners working in biotechnology, pharmaceutical companies, food industries, and those trained in molecular biology and cell manipulation, who need to acquire knowledge on the principles of large scale processing of biological material.

Table of Contents

Preface to the Second Edition

Part 1 Introduction

Chapter 1. Bioprocess Development

1.1 Steps in Bioprocess Development: A Typical New Product from Recombinant DNA

1.2 A Quantitative Approach

Chapter 2. Introduction to Engineering Calculations

2.1 Physical Variables, Dimensions, and Units

2.2 Units

2.3 Force and Weight

2.4 Measurement Conventions

2.5 Standard Conditions and Ideal Gases

2.6 Physical and Chemical Property Data

2.7 Stoichiometry

2.8 Methods for Checking and Estimating Results

Summary of Chapter 2


Suggestions for Further Reading

Chapter 3. Presentation and Analysis of Data

3.1 Errors in Data and Calculations

3.2 Presentation of Experimental Data

3.3 Data Analysis

3.4 Graph Paper with Logarithmic Coordinates

3.5 General Procedures for Plotting Data

3.6 Process Flow Diagrams

Summary of Chapter 3


Suggestions for Further Reading

Part 2 Material and Energy Balances

Chapter 4. Material Balances

4.1 Thermodynamic Preliminaries

4.2 Law of Conservation of Mass

4.3 Procedure for Material Balance Calculations

4.4 Material Balance Worked Examples

4.5 Material Balances with Recycle, Bypass, and Purge Streams

4.6 Stoichiometry of Cell Growth and Product Formation

Summary of Chapter 4


Suggestions for Further Reading

Chapter 5. Energy Balances

5.1 Basic Energy Concepts

5.2 General Energy Balance Equations

5.3 Enthalpy Calculation Procedures

5.4 Enthalpy Change in Nonreactive Processes

5.5 Steam Tables

5.6 Procedure for Energy Balance Calculations without Reaction

5.7 Energy Balance Worked Examples without Reaction

5.8 Enthalpy Change Due to Reaction

5.9 Heat of R


No. of pages:
© 2012
Academic Press
Print ISBN:
Electronic ISBN:


"Doran (Swinburne U. of Technology, Australia) revises her 1995 graduate and undergraduate textbook for students of molecular biology, biotechnology, and related disciples who have little or no engineering background but want to become familiar with the processes by which biological discoveries are transformed into commercial products. Her emphasis is on the underlying scientific and engineering principles rather than on specific biotechnology applications, she says, so most of the material in the first edition remains relevant. There have been some significant developments, however, and she includes new sections on sustainable bioprocessing and metabolic engineering. Overall, she covers introductory matters, material and energy balances, physical processes, and reactions and reactors."--Reference & Research Book News October 2012