The emergence and refinement of techniques in molecular biology has changed our perceptions of medicine, agriculture, and environmental management. Scientific breakthroughs in gene expression, protein engineering, and cell fusion are being translated by a strengthening biotechnology industry into revolutionary new products and services. Many a student has been enticed by the promise of biotechnology and the excitement of being near the cutting edge of scientific advancement. However, graduates trained in molecular biology and cell manipulation soon realize that these techniques are only part of the picture. Reaping the full benefits of biotechnology requires manufacturing capability involving the large-scale processing of biological material. Increasingly, biotechnologists are being employed by companies to work in cooperation with chemical engineers to achieve pragmatic commercial goals. For many years, aspects of biochemistry and molecular genetics have been included in chemical engineering curricula, yet there has been little attempt until recently to teach aspects of engineering applicable to process design to biotechnologists. This textbook is the first to present the principles of bioprocess engineering in a way that is accessible to biological scientists. Other texts on bioprocess engineering currently available assume that the reader already has engineering training. On the other hand, chemical engineering textbooks do not consider examples from bioprocessing, and are written almost exclusively with the petroleum and chemical industries in mind. This publication explains process analysis from an engineering point of view, but refers exclusively to the treatment of biological systems. Over 170 problems and worked examples encompass a wide range of applications, including recombinant plant and animal cell cultures, immobilized catalysts, as well as traditional fermentation systems.

Key Features

@introbul:Key Features @bul:* First book to present the principles of bioprocess engineering in a way that is accessible to biological scientists * Explains process analysis from an engineering point of view, but uses worked exam


@aud:AUDIENCE: Senior undergraduate students in applied biology taking final year options in bioprocessing/biotechnology and graduate students in biological sciences taking masters courses, or beginning research in engineering departments. This book shoul

Table of Contents

Introduction: Bioprocess Development: An Interdisciplinary Challenge: Steps in Bioprocess Development: A Typical New Product from Recombinant DNA. A Quantitative Approach. Introduction to Engineering Calculations: Physical Variables, Dimensions and Units. Units. Force and Weight. Measurement Conventions. Standard Conditions and Ideal Gases. Physical and Chemical Property Data. Stoichiometry. Presentation and Analysis of Data: Errors in Data and Calculations. Presentation of Experimental Data. Data Analysis. Graph Paper with Logarithmic Coordinates. General Procedures for Plotting Data. Process Flow Diagrams. Material and Energy Balances: Material Balances: Thermodynamic Preliminaries. Law of Conservation of Mass. Procedure for Material-Balance Calculations. Material-Balance Worked Examples. Material Balances with Recycle, By-Pass and Purge Streams. Stoichiometry of Growth and Product Formation. Energy Balances: Basic Energy Concepts. General Energy-Balance Equations. Enthalpy Calculation Procedures. Enthalpy Change in Non-Reactive Processes. Steam Tables. Procedure for Energy-Balance Calculations without Reaction. Energy-Balance Worked Examples without Reaction. Enthalpy Change Due to Reaction. Heat of Reaction for Processes with Biomass Production. Energy-Balance Equation for Cell Culture. Fermentation Energy-Balance Worked Examples. Unsteady-


No. of pages:
© 1995
Academic Press
Print ISBN:
Electronic ISBN:

About the author

Pauline Doran

Affiliations and Expertise

Swinburne University of Technology, Faculty of Science, Engineering and Technology, School of Science, Department of Chemistry and Biotechnology


From the Prepublication Reviews: "In general the text is very well written, well organized and is of high quality in all respects.... The emphasis on physical explanations that accompany the equations is very helpful.... The coverage is very complete and comprehensive." -DAVID DIBIASIO, WORCESTER POLYTECHNIC INSTITUTE, MASSACHUSETTS, USA