Bioprocess Engineering Principles

Bioprocess Engineering Principles

2nd Edition - March 27, 2012

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  • Author: Pauline Doran
  • Paperback ISBN: 9780122208515
  • eBook ISBN: 9780080917702

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This welcome new edition discusses bioprocess engineering from the perspective of biology students. It includes a great deal of new material and has been extensively revised and expanded. These updates strengthen the book and maintain its position as the book of choice for senior undergraduates and graduates seeking to move from biochemistry/microbiology/molecular biology to bioprocess engineering.

Key Features

  • All chapters thoroughly revised for current developments, with over 200 pgs of new material, including significant new content in: Metabolic Engineering, Sustainable Bioprocessing, Membrane Filtration, Turbulence and Impeller Design, Downstream Processing, Oxygen Transfer Systems
  • Over 150 new problems and worked examples
  • More than 100 new illustrations


Senior undergraduate students in applied biology, biomedical engineering, or chemical engineering taking final year options in bioprocessing/biotechnology. Industrial practitioners working in biotechnology, pharmaceutical companies, food industries, and those trained in molecular biology and cell manipulation, who need to acquire knowledge on the principles of large scale processing of biological material

Table of Contents

  • Preface to the Second Edition

    Part 1 Introduction

    Chapter 1. Bioprocess Development

    1.1 Steps in Bioprocess Development: A Typical New Product from Recombinant DNA

    1.2 A Quantitative Approach

    Chapter 2. Introduction to Engineering Calculations

    2.1 Physical Variables, Dimensions, and Units

    2.2 Units

    2.3 Force and Weight

    2.4 Measurement Conventions

    2.5 Standard Conditions and Ideal Gases

    2.6 Physical and Chemical Property Data

    2.7 Stoichiometry

    2.8 Methods for Checking and Estimating Results

    Summary of Chapter 2


    Suggestions for Further Reading

    Chapter 3. Presentation and Analysis of Data

    3.1 Errors in Data and Calculations

    3.2 Presentation of Experimental Data

    3.3 Data Analysis

    3.4 Graph Paper with Logarithmic Coordinates

    3.5 General Procedures for Plotting Data

    3.6 Process Flow Diagrams

    Summary of Chapter 3


    Suggestions for Further Reading

    Part 2 Material and Energy Balances

    Chapter 4. Material Balances

    4.1 Thermodynamic Preliminaries

    4.2 Law of Conservation of Mass

    4.3 Procedure for Material Balance Calculations

    4.4 Material Balance Worked Examples

    4.5 Material Balances with Recycle, Bypass, and Purge Streams

    4.6 Stoichiometry of Cell Growth and Product Formation

    Summary of Chapter 4


    Suggestions for Further Reading

    Chapter 5. Energy Balances

    5.1 Basic Energy Concepts

    5.2 General Energy Balance Equations

    5.3 Enthalpy Calculation Procedures

    5.4 Enthalpy Change in Nonreactive Processes

    5.5 Steam Tables

    5.6 Procedure for Energy Balance Calculations without Reaction

    5.7 Energy Balance Worked Examples without Reaction

    5.8 Enthalpy Change Due to Reaction

    5.9 Heat of Reaction for Processes with Biomass Production

    5.10 Energy Balance Equation for Cell Culture

    5.11 Cell Culture Energy Balance Worked Examples

    Summary of Chapter 5


    Suggestions for Further Reading

    Chapter 6. Unsteady-State Material and Energy Balances

    6.1 Unsteady-State Material Balance Equations

    6.2 Unsteady-State Energy Balance Equations

    6.3 Solving Differential Equations

    6.4 Solving Unsteady-State Mass Balances

    6.5 Solving Unsteady-State Energy Balances

    Summary of Chapter 6


    Suggestions for Further Reading

    Part 3 Physical Processes

    Chapter 7. Fluid Flow

    7.1 Classification of Fluids

    7.2 Fluids in Motion

    7.3 Viscosity

    7.4 Momentum Transfer

    7.5 Non-Newtonian Fluids

    7.6 Viscosity Measurement

    7.7 Rheological Properties of Fermentation Broths

    7.8 Factors Affecting Broth Viscosity

    7.9 Turbulence

    Summary of Chapter 7


    Suggestions for Further Reading

    Chapter 8. Mixing

    8.1 Functions of Mixing

    8.2 Mixing Equipment

    8.3 Flow Patterns in Stirred Tanks

    8.4 Impellers

    8.5 Stirrer Power Requirements

    8.6 Power Input by Gassing

    8.7 Impeller Pumping Capacity

    8.8 Suspension of Solids

    8.9 Mechanisms of Mixing

    8.10 Assessing Mixing Effectiveness

    8.11 Scale-Up of Mixing Systems

    8.12 Improving Mixing in Fermenters

    8.13 Multiple Impellers

    8.14 Retrofitting

    8.15 Effect of Rheological Properties on Mixing

    8.16 Role of Shear in Stirred Fermenters

    Summary of Chapter 8


    Suggestions for Further Reading

    Chapter 9. Heat Transfer

    9.1 Heat Transfer Equipment

    9.2 Mechanisms of Heat Transfer

    9.3 Conduction

    9.4 Heat Transfer between Fluids

    9.5 Design Equations for Heat Transfer Systems

    9.6 Application of the Design Equations

    9.7 Hydrodynamic Considerations with Cooling Coils

    Summary of Chapter 9


    Suggestions for Further Reading

    Chapter 10. Mass Transfer

    10.1 Molecular Diffusion

    10.2 Role of Diffusion in Bioprocessing

    10.3 Film Theory

    10.4 Convective Mass Transfer

    10.5 Oxygen Uptake in Cell Cultures

    10.6 Factors Affecting Oxygen Transfer in Fermenters

    10.7 Measuring Dissolved Oxygen Concentration

    10.8 Estimating Oxygen Solubility

    10.9 Mass Transfer Correlations for Oxygen Transfer

    10.10 Measurement of kLa

    10.11 Measurement of the Specific Oxygen Uptake Rate, qO

    10.12 Practical Aspects of Oxygen Transfer in Large Fermenters

    10.13 Alternative Methods for Oxygenation without Sparging

    10.14 Oxygen Transfer in Shake Flasks

    Summary of Chapter 10


    Suggestions for Further Reading

    Chapter 11. Unit Operations

    11.1 Overview of Downstream Processing

    11.2 Overview of Cell Removal Operations

    11.3 Filtration

    11.4 Centrifugation

    11.5 Cell Disruption

    11.6 The Ideal Stage Concept

    11.7 Aqueous Two-Phase Liquid Extraction

    11.8 Precipitation

    11.9 Adsorption

    11.10 Membrane Filtration

    11.11 Chromatography

    11.12 Crystallisation

    11.13 Drying

    Summary of Chapter 11


    Suggestions for Further Reading

    Part 4 Reactions and Reactors

    Chapter 12. Homogeneous Reactions

    12.1 Basic Reaction Theory

    12.2 Calculation of Reaction Rates from Experimental Data

    12.3 General Reaction Kinetics for Biological Systems

    12.4 Determining Enzyme Kinetic Constants from Batch Data

    12.5 Regulation of Enzyme Activity

    12.6 Kinetics of Enzyme Deactivation

    12.7 Yields in Cell Culture

    12.8 Cell Growth Kinetics

    12.9 Growth Kinetics with Plasmid Instability

    12.10 Production Kinetics in Cell Culture

    12.11 Kinetics of Substrate Uptakein Cell Culture

    12.12 Effect of Culture Conditions on Cell Kinetics

    12.13 Determining Cell Kinetic Parameters from Batch Data

    12.14 Effect of Maintenance on Yields

    12.15 Kinetics of Cell Death

    12.16 Metabolic Engineering

    Summary of Chapter 12


    Suggestions for Further Reading

    Chapter 13. Heterogeneous Reactions

    13.1 Heterogeneous Reactions in Bioprocessing

    13.2 Concentration Gradients and Reaction Rates in Solid Catalysts

    13.3 Internal Mass Transfer and Reaction

    13.4 The Thiele Modulus and Effectiveness Factor

    13.5 External Mass Transfer

    13.6 Liquid–Solid Mass Transfer Correlations

    13.7 Experimental Aspects

    13.8 Minimising Mass Transfer Effects

    13.9 Evaluating True Kinetic Parameters

    13.10 General Comments on Heterogeneous Reactions in Bioprocessing

    Summary of Chapter 13


    Suggestions for Further Reading

    Chapter 14. Reactor Engineering

    14.1 Bioreactor Engineering in Perspective

    14.2 Bioreactor Configurations

    14.3 Practical Considerations for Bioreactor Construction

    14.4 Monitoring and Control of Bioreactors

    14.5 Ideal Reactor Operation

    14.6 Sterilisation

    14.7 Sustainable Bioprocessing

    Summary of Chapter 14


    Suggestions for Further Reading


    APPENDIX A. Conversion Factors

    APPENDIX B. Ideal Gas Constant

    APPENDIX C. Physical and Chemical Property Data

    APPENDIX D. Steam Tables

    APPENDIX E. Mathematical Rules

    APPENDIX F. U.S. Sieve and Tyler Standard Screen Series


Product details

  • No. of pages: 926
  • Language: English
  • Copyright: © Academic Press 2012
  • Published: March 27, 2012
  • Imprint: Academic Press
  • Paperback ISBN: 9780122208515
  • eBook ISBN: 9780080917702

About the Author

Pauline Doran

Pauline M. Doran Swinburne University of Technology, Faculty of Science, Engineering and Technology, School of Science, Department of Chemistry and Biotechnology. Professor Doran has taught bioprocess engineering and biotechnology at undergraduate and graduate levels for more than 30 years. Her most significant contributions to the field include bioreactor design and analysis for plant organ culture, foreign protein production in plant systems, and human tissue engineering using stem cells.

Affiliations and Expertise

Swinburne University of Technology, Faculty of Science, Engineering and Technology, School of Science, Department of Chemistry and Biotechnology

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