Biophysical Tools for Biologists

Biophysical Tools for Biologists

In Vivo Techniques

1st Edition - November 3, 2008

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  • Editors: John Correia, H. Detrich, III
  • eBook ISBN: 9780080919782
  • Hardcover ISBN: 9780123725219

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Driven in part by the development of genomics, proteomics, and bioinformatics as new disciplines, there has been a tremendous resurgence of interest in physical methods to investigate macromolecular structure and function in the context of living cells. This volume in Methods in Cell Biology is devoted to biophysical techniques in vivo and their applications to cellular biology. Biophysical Tools for Biologists covers methods-oriented chapters on fundamental as well as cutting-edge techniques in molecular and cellular biophysics. This book is directed toward the broad audience of cell biologists, biophysicists, pharmacologists, and molecular biologists who employ classical and modern biophysical technologies or wish to expand their expertise to include such approaches. It will also interest the biomedical and biotechnology communities for biophysical characterization of drug formulations prior to FDA approval.

Key Features

  • Describes techniques in the context of important biological problems
  • Delineates critical steps and potential pitfalls for each method


Cell biologists, biophysicists, pharmacologists, and molecular biologists

Table of Contents

  • Section 1. Fluorescence Methods
    1) Photoactivation and Photobleaching Techniques for Analysis of Organelle Biogenesis in vivo
    2) Analysis of the Dynamics of Living Cells by Fluorescence Correlation Spectroscopy
    3) Molecular Sensors Based on Fluorescence Resonance Energy Transfer to Visualize Cellular Dynamics 4) Real-Time Fluorescence of Protein Folding in vivo
    5) Microfluidic Glucose Stimulation of Ca+2 Oscillations in Pancreatic Islets

    Section 2. Microscopic Methods
    6) Introduction to Optical Sectioning: Confocal, Deconvolution, and Two-Photon
    7) Use of Electron Tomography to Elucidate Sub-Cellular Structure and Function
    8) Proteomics of Macromolecular Complexes by Cellular Cryo-Electron Tomography
    9) Total Internal Reflectance Microscopy (TIRF)
    10) Atomic Force Microscopy of Living Cells
    11) Real-Time Kinetics of Gene Activity in Individual Bacteria
    12) Measurement of Cytoskeletal Proteins Globally and Locally in vivo
    13) Infrared and Raman Microscopy in Cell Biology
    14) Imaging Fluorescent Mice in vivo by Confocal Microscopy
    15) Nanoscale Imaging of Intracellular Fluorescent Proteins: Breaking the Diffraction Barrier

    Section 3. Methods at the In Vitro/In Vivo Interface
    16) Analysis of Protein Posttranslational Modification by Mass Spectrometry
    17) Imaging Mass Spectrometry
    18) Wet EM Using Quantum Dots
    19) Single Cell Capillary Electrophoresis

    Section 4. Methods for Diffusion, Viscosity, Force and Displacement
    20) Single-Molecule Force Spectroscopy in Living Cells
    21) Magnetic Bead Force Applications
    22) Measurement of Membrane-Cytoskeleton Adhesion Using Laser Optical Tweezers
    23) Cellular Rheological Measurements in vivo
    24) Physical Behavior of Cytoskeletal Networks in vitro and in vivo
    25) Force Regulation of Microtubule Dynamics in Fission Yeast

    Section 5. Techniques for Protein Activity, Protein-Protein and Protein-RNA Interactions
    26) Quantifying Protein Activity Using FRET and FLIM Microscopy
    27) Measurement of Protein-Protein Interactions in vivo Using FRET and FLIM
    28) Measurement of RNA Interactions in vivo Using Molecular Beacons –

    Section 6. Computational Modeling
    29) Stochastic Modeling in Cell Biology
    30) Computational Methods for Analyzing Patterns in Dynamic Biological Phenomena: An Application to Microtubule Dynamics
    31) Computational Modeling of Self-Organized Spindle Formation

Product details

  • No. of pages: 704
  • Language: English
  • Copyright: © Academic Press 2008
  • Published: November 3, 2008
  • Imprint: Academic Press
  • eBook ISBN: 9780080919782
  • Hardcover ISBN: 9780123725219

About the Serial Volume Editors

John Correia

Affiliations and Expertise

University of Mississippi Medical Center, Jackson, USA

H. Detrich, III

Professor of Biochemistry and Marine Biology at Northeastern University, promoted 1996. Joined Northeastern faculty in 1987. Previously a faculty member in Dept. of Biochemistry at the University of Mississippi Medical Center, 1983-1987.Principal Investigator in the U.S. Antarctic Program since 1984. Twelve field seasons "on the ice" since 1981. Research conducted at Palmer Station, Antarctica, and McMurdo Station, Antarctica.Research areas: Biochemical, cellular, and physiological adaptation to low and high temperatures. Structure and function of cytoplasmic microtubules and microtubule-dependent motors from cold-adapted Antarctic fishes. Regulation of tubulin and globin gene expression in zebrafish and Antarctic fishes. Role of microtubules in morphogenesis of the zebrafish embryo. Developmental hemapoiesis in zebrafish and Antarctic fishes. UV-induced DNA damage and repair in Antarctic marine organisms.

Affiliations and Expertise

Northeastern University, Boston, MA, USA

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