Biophysical Tools for Biologists

Biophysical Tools for Biologists

In Vitro Techniques

1st Edition - November 5, 2007
  • Editors: John Correia, H. Detrich, III
  • eBook ISBN: 9780080554945
  • Hardcover ISBN: 9780123725202

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Description

Driven in part by the development of genomics, proteomics, and bioinformatics as new disciplines, there has been a tremendous resurgence of interest in physical methods to investigate macromolecular structure and function in the context of living cells. This volume in Methods in Cell Biology is devoted to biophysical techniques in vitro and their applications to cellular biology. Biophysical Tools for Biologists covers methods-oriented chapters on fundamental as well as cutting-edge techniques in molecular and cellular biophysics. This book is directed toward the broad audience of cell biologists, biophysicists, pharmacologists, and molecular biologists who employ classical and modern biophysical technologies or wish to expand their expertise to include such approaches. It will also interest the biomedical and biotechnology communities for biophysical characterization of drug formulations prior to FDA approval.

Key Features

  • Describes techniques in the context of important biological problems
  • Delineates critical steps and potential pitfalls for each method
  • Includes full-color plates to illustrate techniques

Readership

Cell biologists, biophysicists, pharmacologists, and molecular biologists.

Table of Contents

  • Part I: History and Reflections

    Section 1. Solution Methods

    1. Binding: A Polemic and Rough Guide
    Nichola C. Garbett, Jonathan B. Chaires

    2. Linked Equilibria in Regulation of Transcription Initiation
    Dorothy Beckett

    3. Biosensor-Surface Plasmon Resonance Methods for Quantitative Analysis of Biomolecular Interactions
    Farial A. Tanious, Binh Nguyen, and W. David Wilson

    4. Isothermal Titration Calorimetry: Experimental Design, Data Analysis, and Probing Macromolecule/Ligand Binding and Kinetic Interactions
    Matthew W. Freyer and Edwin A. Lewis

    5. Differential Scanning Calorimetry
    Charles H. Spink

    6. Analytical Ultracentrifugation: A) Sedimentation Velocity, B) Sedimentation Equilibrium
    Tom Laue, Tom Moody, James Cole, and Jeff Lary

    7. Determination of membrane protein molecular weights and association equilibrium constants using sedimentation equilibrium and sedimentation velocity
    Nancy K. Burgess, Ann Marie Stanley, and Karen G. Fleming

    8. Basic Aspects of Absorption and Fluorescence Spectroscopy and Resonance Energy Transfer Methods
    Susan Bane and Natasha Shanker

    9. Applications of Fluorescence Anisotropy to the Study of Protein-DNA Interactions
    Vince J. LiCata and Andy J. Wowor

    10. Circular Dichroism and Its Application to the Study of Biomolecules
    Stephen R. Martin and Maria J. Schilstra

    11. Folding and Stability
    Timothy O. Street, Naomi Courtemanche and Doug Barrick

    12. Hydrodynamic shape modelling of analytical ultracentrifugation data
    Olwyn Byron

    13. X-ray and neutron scattering data and their constrained molecular modelling
    Stephen J Perkins, Azubuike I. Okemefuna, Anira N. Fernando, Alexandra Bonner, Hannah E. Gilbert and Patricia B. Furtado

    14. Structural Investigations into Microtubule-MAP Complexes
    Andreas Hoenger and Heinz Gross

    15. Rapid Kinetic Techniques
    John F. Eccleston, Stephen Martin, and Maria J. Schilstra

    16. Mutagenic Analysis of the Membrane Protein Functional Mechanisms: Bacteriorhodopsin as a Model Example
    George J. Turner

    17. Quantifying DNA-protein interactions by single molecule stretching
    Mark C. Williams, Ioulia Rouzina, and Richard L. Karpel

    18. Isotopomer-based metabolomic analysis by NMR and mass spectrometry
    Andrew N Lane, Teresa W-M. Fan, and Richard M. Higashi

    19. Following molecular transitions with single residue spatial
    and millisecond time resolution
    Inna Shcherbakova, Somdeb Mitra, Robert Beer and Michael Brenowitz

    20. Methods and applications of site-directed spin labeling EPR spectroscopy
    Candice S. Klug and Jimmy B. Feix

    21. Fluorescence Correlation Spectroscopy and its application to the characterization of molecular properties and interactions
    Hacène Boukari and Dan L. Sackett

    22. Practical Guide to Osmolytes
    Jorg Rosgen and Daniel Harries

    23. Stupid Statistics
    Joel Tellinghuisen

    24. Nonlinear Least Squares Fitting Methods
    Michael L. Johnson

    25. Methods for Simulating the Dynamics of Complex Biological Processes
    Maria J Schilstra, Stephen R. Martin, and Sarah M. Keating

    26. Computational Methods for Biomolecular Electrostatics
    Feng Dong, Brett Olsen and Nathan A. Baker

    27. Ligand effects on the protein ensemble: Unifying the descriptions of ligand binding, local conformational fluctuations, and protein stability
    Steven T Whitten, Bertrand García-Moreno E., and Vincent J. Hilser

    28. Molecular Modeling of Cytoskeletal Proteins
    Xiange Zheng and David Sept

    29. Mathematical Modeling of Cell Motility
    Anders E. Carlsson and David Sept

Product details

  • No. of pages: 1014
  • Language: English
  • Copyright: © Academic Press 2007
  • Published: November 5, 2007
  • Imprint: Academic Press
  • eBook ISBN: 9780080554945
  • Hardcover ISBN: 9780123725202

About the Editors

John Correia

Affiliations and Expertise

University of Mississippi Medical Center, Jackson, USA

H. Detrich, III

Professor of Biochemistry and Marine Biology at Northeastern University, promoted 1996. Joined Northeastern faculty in 1987. Previously a faculty member in Dept. of Biochemistry at the University of Mississippi Medical Center, 1983-1987.Principal Investigator in the U.S. Antarctic Program since 1984. Twelve field seasons "on the ice" since 1981. Research conducted at Palmer Station, Antarctica, and McMurdo Station, Antarctica.Research areas: Biochemical, cellular, and physiological adaptation to low and high temperatures. Structure and function of cytoplasmic microtubules and microtubule-dependent motors from cold-adapted Antarctic fishes. Regulation of tubulin and globin gene expression in zebrafish and Antarctic fishes. Role of microtubules in morphogenesis of the zebrafish embryo. Developmental hemapoiesis in zebrafish and Antarctic fishes. UV-induced DNA damage and repair in Antarctic marine organisms.

Affiliations and Expertise

Northeastern University, Boston, MA, USA