Description

Driven in part by the development of genomics, proteomics, and bioinformatics as new disciplines, there has been a tremendous resurgence of interest in physical methods to investigate macromolecular structure and function in the context of living cells. This volume in Methods in Cell Biology is devoted to biophysical techniques in vitro and their applications to cellular biology. The volume covers methods-oriented chapters on fundamental as well as cutting-edge techniques in molecular and cellular biophysics. This book is directed toward the broad audience of cell biologists, biophysicists, pharmacologists, and molecular biologists who employ classical and modern biophysical technologies or wish to expand their expertise to include such approaches. It will also interest the biomedical and biotechnology communities for biophysical characterization of drug formulations prior to FDA approval.

Key Features

* Describes techniques in the context of important biological problems * Delineates critical steps and potential pitfalls for each method * Includes full-color plates to illustrate techniques

Readership

Cell biologists, biophysicists, pharmacologists, and molecular biologists.

Table of Contents

Part I: History and Reflections Section 1. Solution Methods 1. Binding: A Polemic and Rough Guide Nichola C. Garbett, Jonathan B. Chaires 2. Linked Equilibria in Regulation of Transcription Initiation Dorothy Beckett 3. Biosensor-Surface Plasmon Resonance Methods for Quantitative Analysis of Biomolecular Interactions Farial A. Tanious, Binh Nguyen, and W. David Wilson 4. Isothermal Titration Calorimetry: Experimental Design, Data Analysis, and Probing Macromolecule/Ligand Binding and Kinetic Interactions Matthew W. Freyer and Edwin A. Lewis 5. Differential Scanning Calorimetry Charles H. Spink 6. Analytical Ultracentrifugation: A) Sedimentation Velocity, B) Sedimentation Equilibrium Tom Laue, Tom Moody, James Cole, and Jeff Lary 7. Determination of membrane protein molecular weights and association equilibrium constants using sedimentation equilibrium and sedimentation velocity Nancy K. Burgess, Ann Marie Stanley, and Karen G. Fleming 8. Basic Aspects of Absorption and Fluorescence Spectroscopy and Resonance Energy Transfer Methods Susan Bane and Natasha Shanker 9. Applications of Fluorescence Anisotropy to the Study of Protein-DNA Interactions Vince J. LiCata and Andy J. Wowor 10. Circular Dichroism and Its Application to the Study of Biomolecules Stephen R. Martin and Maria J. Schilstra 11. Folding and Stability Timothy O. Street, Naomi Courtemanche and Doug Barrick 12. Hydrodynamic shape modelling of analytical ultracentrifugation data Olwyn Byron

Details

No. of pages:
1014
Language:
English
Copyright:
© 2007
Published:
Imprint:
Academic Press
Electronic ISBN:
9780080554945
Print ISBN:
9780123725202

About the editors

H. William Detrich, III

Professor of Biochemistry and Marine Biology at Northeastern University, promoted 1996. Joined Northeastern faculty in 1987. Previously a faculty member in Dept. of Biochemistry at the University of Mississippi Medical Center, 1983-1987.Principal Investigator in the U.S. Antarctic Program since 1984. Twelve field seasons "on the ice" since 1981. Research conducted at Palmer Station, Antarctica, and McMurdo Station, Antarctica.Research areas: Biochemical, cellular, and physiological adaptation to low and high temperatures. Structure and function of cytoplasmic microtubules and microtubule-dependent motors from cold-adapted Antarctic fishes. Regulation of tubulin and globin gene expression in zebrafish and Antarctic fishes. Role of microtubules in morphogenesis of the zebrafish embryo. Developmental hemapoiesis in zebrafish and Antarctic fishes. UV-induced DNA damage and repair in Antarctic marine organisms.