Biophysical Tools for Biologists
In Vitro Techniques
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Driven in part by the development of genomics, proteomics, and bioinformatics as new disciplines, there has been a tremendous resurgence of interest in physical methods to investigate macromolecular structure and function in the context of living cells. This volume in Methods in Cell Biology is devoted to biophysical techniques in vitro and their applications to cellular biology. Biophysical Tools for Biologists covers methods-oriented chapters on fundamental as well as cutting-edge techniques in molecular and cellular biophysics. This book is directed toward the broad audience of cell biologists, biophysicists, pharmacologists, and molecular biologists who employ classical and modern biophysical technologies or wish to expand their expertise to include such approaches. It will also interest the biomedical and biotechnology communities for biophysical characterization of drug formulations prior to FDA approval.
Key Features
- Describes techniques in the context of important biological problems
- Delineates critical steps and potential pitfalls for each method
- Includes full-color plates to illustrate techniques
Readership
Cell biologists, biophysicists, pharmacologists, and molecular biologists.
Table of Contents
- Part I: History and Reflections
Section 1. Solution Methods
1. Binding: A Polemic and Rough Guide
Nichola C. Garbett, Jonathan B. Chaires
2. Linked Equilibria in Regulation of Transcription Initiation
Dorothy Beckett
3. Biosensor-Surface Plasmon Resonance Methods for Quantitative Analysis of Biomolecular Interactions
Farial A. Tanious, Binh Nguyen, and W. David Wilson
4. Isothermal Titration Calorimetry: Experimental Design, Data Analysis, and Probing Macromolecule/Ligand Binding and Kinetic Interactions
Matthew W. Freyer and Edwin A. Lewis
5. Differential Scanning Calorimetry
Charles H. Spink
6. Analytical Ultracentrifugation: A) Sedimentation Velocity, B) Sedimentation Equilibrium
Tom Laue, Tom Moody, James Cole, and Jeff Lary
7. Determination of membrane protein molecular weights and association equilibrium constants using sedimentation equilibrium and sedimentation velocity
Nancy K. Burgess, Ann Marie Stanley, and Karen G. Fleming
8. Basic Aspects of Absorption and Fluorescence Spectroscopy and Resonance Energy Transfer Methods
Susan Bane and Natasha Shanker
9. Applications of Fluorescence Anisotropy to the Study of Protein-DNA Interactions
Vince J. LiCata and Andy J. Wowor
10. Circular Dichroism and Its Application to the Study of Biomolecules
Stephen R. Martin and Maria J. Schilstra
11. Folding and Stability
Timothy O. Street, Naomi Courtemanche and Doug Barrick
12. Hydrodynamic shape modelling of analytical ultracentrifugation data
Olwyn Byron
13. X-ray and neutron scattering data and their constrained molecular modelling
Stephen J Perkins, Azubuike I. Okemefuna, Anira N. Fernando, Alexandra Bonner, Hannah E. Gilbert and Patricia B. Furtado
14. Structural Investigations into Microtubule-MAP Complexes
Andreas Hoenger and Heinz Gross
15. Rapid Kinetic Techniques
John F. Eccleston, Stephen Martin, and Maria J. Schilstra
16. Mutagenic Analysis of the Membrane Protein Functional Mechanisms: Bacteriorhodopsin as a Model Example
George J. Turner
17. Quantifying DNA-protein interactions by single molecule stretching
Mark C. Williams, Ioulia Rouzina, and Richard L. Karpel
18. Isotopomer-based metabolomic analysis by NMR and mass spectrometry
Andrew N Lane, Teresa W-M. Fan, and Richard M. Higashi
19. Following molecular transitions with single residue spatial
and millisecond time resolution
Inna Shcherbakova, Somdeb Mitra, Robert Beer and Michael Brenowitz
20. Methods and applications of site-directed spin labeling EPR spectroscopy
Candice S. Klug and Jimmy B. Feix
21. Fluorescence Correlation Spectroscopy and its application to the characterization of molecular properties and interactions
Hacène Boukari and Dan L. Sackett
22. Practical Guide to Osmolytes
Jorg Rosgen and Daniel Harries
23. Stupid Statistics
Joel Tellinghuisen
24. Nonlinear Least Squares Fitting Methods
Michael L. Johnson
25. Methods for Simulating the Dynamics of Complex Biological Processes
Maria J Schilstra, Stephen R. Martin, and Sarah M. Keating
26. Computational Methods for Biomolecular Electrostatics
Feng Dong, Brett Olsen and Nathan A. Baker
27. Ligand effects on the protein ensemble: Unifying the descriptions of ligand binding, local conformational fluctuations, and protein stability
Steven T Whitten, Bertrand García-Moreno E., and Vincent J. Hilser
28. Molecular Modeling of Cytoskeletal Proteins
Xiange Zheng and David Sept
29. Mathematical Modeling of Cell Motility
Anders E. Carlsson and David Sept
Product details
- No. of pages: 1014
- Language: English
- Copyright: © Academic Press 2007
- Published: November 5, 2007
- Imprint: Academic Press
- eBook ISBN: 9780080554945
- Hardcover ISBN: 9780123725202
About the Editors
John Correia
Affiliations and Expertise
University of Mississippi Medical Center, Jackson, USA
H. Detrich, III
Professor of Biochemistry and Marine Biology at Northeastern University, promoted 1996. Joined Northeastern faculty in 1987. Previously a faculty member in Dept. of Biochemistry at the University of Mississippi Medical Center, 1983-1987.Principal Investigator in the U.S. Antarctic Program since 1984. Twelve field seasons "on the ice" since 1981. Research conducted at Palmer Station, Antarctica, and McMurdo Station, Antarctica.Research areas: Biochemical, cellular, and physiological adaptation to low and high temperatures. Structure and function of cytoplasmic microtubules and microtubule-dependent motors from cold-adapted Antarctic fishes. Regulation of tubulin and globin gene expression in zebrafish and Antarctic fishes. Role of microtubules in morphogenesis of the zebrafish embryo. Developmental hemapoiesis in zebrafish and Antarctic fishes. UV-induced DNA damage and repair in Antarctic marine organisms.
Affiliations and Expertise
Northeastern University, Boston, MA, USA