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Biomembranes, Part V: Cellular and Subcellular Transport: Epithelial Cells - 1st Edition - ISBN: 9780121820923, 9780080883083

Biomembranes, Part V: Cellular and Subcellular Transport: Epithelial Cells, Volume 191

1st Edition

Editors in Chief: John Abelson Melvin Simon
Serial Volume Editors: Sidney Fleischer Becca Fleischer
eBook ISBN: 9780080883083
Imprint: Academic Press
Published Date: 28th December 1990
Page Count: 939


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Table of Contents

Epithelial Transport: An Introduction, K.J. Ullrich. G. Kottra and E. Fromter, Determination of Paracellular Shunt Conductances in Epithelia. KIDNEY: Intact Organ: P. Silva, Isolated Perfused and Nonfiltering Kidney. C.J. Lumsden and M. Silverman, Multiple Indicator Dilution and the Kidney: Kinetics, Permeation, and Transport in Vivo. I.M. Shafik and G.A. Quamme, Micropuncture Techniques in Renal Research. K.J. Ullrich and G. Rumrich, Kidney: Microperfusion - Double-Perfused Tubule in Situ. Glomerular Filtration: A. Yared and I. Ichikawa, Transcapillary Fluid Transport in Glomerulus. D. Schlondorff, Preparation and Study of Isolated Glomeruli. D.A. Troyer and J.I. Kreisberg, Isolation and Study of Glomerular Cells. E.E. Muirhead, W.A. Rightsel, J.A. Pitcock, and T. Inagami, Isolation and Culture of Juxtaglomerular and Renomedullary Interstitial Cells. Isolated Tubules: K.W. Beyenbach and W.H. Dantzler, Comparative Kidney Tubule Sources, Isolation, Perfusion, and Function. P.A. Wright, M.B. Burg, and, M.A. Knepper, Microdissection of Kidney Tubule Segments. J.C. Williams, Jr., and J.A. Schafer, Measurement of Transmural Water Flow in Isolated Pewrfused Tubule Segments. Q. Al-Awqati and G.J. Schwartz, Use of Fluorescent Dyes to Identify Structure and Function of Renal Tubular Cells. B. Caissling and M. Le Hir, Functional Morphology of Kidney Cells and Tubules in Situ. R. Greger, Electrophysiological Approach to Study Isolated Perfused Tubule. F. Morel and D. Butlen, Hormonal Receptors in Isolated Tubule. G. Wirthensohn and W.G. Guder, Metabolism of Isolated Kidney Tubule Segments. T. Maack and C.H. Park, Endocytosis and Lysosomal Hydrolysis of Proteins in Proximal Tubules. J.A. Schafer and J.C. Williams, Jr. , Flux Measurements in Isolated Perfused Tubules. W.B. Guggino, Measurement of Volume and Shape Changes in Isolated Tubules. Isolated Renal Cells: R. Kinne, Transport in Isolated Cells from Defined Nephron Segments. M. Horster and J.S. Handler, Primary Culture of Isolated Tubule Cells of Defined Origin. N.L. Simmons, Tissue Culture of Established Renal Cell Lines. H. Oberleithner, A. Schwab, H.-J. Westphale, B. Schuricht, B. Puschel, and H. Koepsell, Giant MDCK Cells: A Novel Expression System. Isolation of Membrane Vesicles: R.K.H. Kinne, Isolation of Luminal and Contraluminal Plasma Membrane Vesicles from Kidney. S. Karlish and G. Sachs, Transport Studies by Optical Methods. R.J. Turner, Stoichiometry of Coupled Transport Systems in Vesicles. J. Biber, K. Malmström, S. Reshkin, and H. Muer, Phosphate Transport in Established Renal Epithelial Cell Lines. I. Sabolic and G. Burckhardt, ATP-Driven Proton Transport in Vesicles from Kidney Cortex. Hormonal Modulation: D. Marver, Aldosterone. D. Brown, A. Verkman, K. Skorecki, and D. Ausiello, Cellular Action of Antidiuretic Hormone.Reconstitution of Transport Proteins. H. Koepsell and S. Seibicke, Reconstitution and Fractionation of Renal Brush-Border Transport Proteins. Stimulus Secretion Coupling in Epithelia: R. Vinayek and J.D. Gardner, Receptor Identification. C.S. Chew, cAMP Technologies, Functional Correlates in Gastric Parietal Cells. H. Rasmussen, Stimulus-Secretion Coupling: General Models and Specific Aspects in Epithelial Cells. L. Hokin, Role of Phosphoinositide-Derived Arachidonic Acid as Second Messenger. J. Williamson, Calcium Signaling Mechanisms. K.A. Wreggett, D.J. Lander, and R.F. Irvine, Two-State Analysis and Radiolabelled Inositol Phosphate Isomers. Pharmacological Agents in Epithelial Transport: B. Wallmark and J. Fryklund, Pharmacological Agents of Gastric Acid Secretion: Receptor Antagonists and Pump Inhibitors. T.R. Kleyman and E.J. Cragoe, Jr. , Cation Transport Probes: Amiloride Series. D.F. Diedrich, Photoaffinity Labeling Analogs of Phlorizin and Phloretin: Synthesis and Effects on Cell Membranes. S.M. O'Grady, M.W. Musch, and M. Field, Diuretic Compounds Structurally Related to Furosemide. R. Greger, Chloride Channel Blockers. Targeting and Intracellular Trafficking in Epithelial Cells: M.L. Bennett, A. Wandinger-Ness, A.W. Brandli, and K. Simons, In Vitro Recovery of Exocytic Transport Vesicles from Polarized MDCK Cells. J.R. Bartles and A.L. Hubbard, Liver: Biogenesis of Rat Hepatocyte Plasma Membrane. R.W. Doms, Oligomerization and Protein Transport. Author Index. Subject Index.


The transport volumes of the Biomembranes series were initiated with Volumes 125 and 126 of Methods in Enzymology, which covered Transport in Bacteria, Mitochondria, and Chloroplasts. Volumes 156 and 157 continued the theme with ATP-Driven Pumps and Related Transport. Cellular and Subcellular Transport: Eukaryotic (Nonepithelial) Cells was the topic of Volumes 173 and 174. The theme of this volume, as well as of Volume 192, is Cellular and Subcellular Transport: Epithelial Cells.


Biochemists, cell biologists, microbiologists, pharmacologists, biomedical scientists, biophysicists, analytical chemists, and physiologists.


No. of pages:
© Academic Press 1990
28th December 1990
Academic Press
eBook ISBN:


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About the Editors in Chief

John Abelson

Affiliations and Expertise

California Institute of Technology, Division of Biology, Pasadena, U.S.A.

Melvin Simon

Affiliations and Expertise

The Salk Institute, La Jolla, CA, USA

About the Serial Volume Editors

Sidney Fleischer

Affiliations and Expertise

Vanderbilt University, Nashville, Tennessee, U.S.A.

Becca Fleischer

Affiliations and Expertise

Vanderbilt University, Nashville, Tennesse, U.S.A.