The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today - truly an essential publication for researchers in all fields of life sciences.


Biochemists, cell biologists, microbiologists, pharmacologists, biomedical scientists, biophysicists, analytical chemists, and physiologists.

Table of Contents

Transport in Subcellular Organelles of Animals: Plasma Membranes and Derived Transporters: D.G. Nicholls, Measurement of Transported Calcium in Synaptosomes. P.E. Kish and T. Ueda, Glutamate Accumulation into Synaptic Vesicles. D. Levy and P. von Dippe, Identification of Bile Acid Transport Protein in Hepatocyte Sinusoidal Plasma Membranes. J.D. McGivan, Transport of Alanine across Hepatocyte Plasma Membrane. S.A. Baldwin and G.E. Lienhard, Purification and Reconstitution of Glucose Transporter from Human Erythrocytes. G.L. Sottocasa, G.C. Lunazzi, and C. Tiribelli, Isolation of Bilitranslocase, the Anion Transporter from Liver Plasma Membrane for Bilirubin and Other Organic Anions. Intracellular Organelles: W.J. Arion, Measurement of Intactness of Rat Liver Endoplasmic Reticulum. N.-E.L. Saris and A. Allshire, Calcium Ion Transport in Mitochondria. D.G. Nicholls and E. Rial, Measurement of Proton Leakage across Mitochondrial Inner Membranes and Its Relation to Protonmotive Force. J.G. McCormack and R.M. Denton, Influence of Calcium Ions on Mammalian Intramitochondrial Dehydrogenases. S. Soboll and H. Sies, Effects of Hormones on Mitochondrial Processes. S. Ohkuma, Use of Fluorescein Isothiocyanate Dextran to Measure Proton Pumping in Lyosomes and Related Organelles. M.L. Smith, A.A. Greene, J.A. Schneider, R.L. Pisoni, and H.N. Christensen, Cystine Exodus from Lyosomes: Cystinosis. U. Hopfer and K. Gasser, Isolation of Physiologically Responsive Secretory Granules from Exocrine Tissues. B. Fleischer, Transport of Nucleotides in Golgi Complex. Transport in Plants:


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© 1989
Academic Press
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@from:Praise for the Series @qu:"The Methods in Enzymology series represents the gold-standard." @source:--NEUROSCIENCE @qu:"Incomparably useful." @source:--ANALYTICAL BIOCHEMISTRY @qu:"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." @source:--BIO/TECHNOLOGY @qu:"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." @source:--CHEMISTRY IN INDUSTRY @qu:"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." @source:--AMERICAN SOCIETY OF MICROBIOLOGY NEWS @qu:"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work." @source:--ENZYMOLOGIA @qu:"A series that has established itself as a definitive reference for biochemists." @source:--JOURNAL OF CHROMATOGRAPHY