The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today - truly an essential publication for researchers in all fields of life sciences.


Biochemists, cell biologists, microbiologists, pharmacologists, biomedical scientists, biophysicsts, analytical chemists, and physiologists.

Table of Contents

Membrane Separation: E. Kinne-Saffran and R.K.H. Kinne, Membrane Isolation: Strategy, Techniques, Markers. A.K. Mircheff, Isolation of Plasma Membranes from Polar Cells and Tissues: Apical/Basolateral Separation, Purity, Function. S.M. Goldin and S.C. King, Transport-Specific Fractionation for Purification of ATP-Dependent Ca2+ Pumps. Q. Al-Awqati, Regulation of Membrane Transport by Endocytotic Removal and Exocytotic Insertion of Transporters. Transport Methods in Cells and Vesicles: H. Rottenberg, Proton Electrochemical Potential Gradient in Vesicles, Organelles, and Prokaryotic Cells. J. van Adelsberg, J. Barasch, and Q. Al-Awqati, Measurement of pH of Intracellular Compartments in Living Cells by Fluorescent Dyes. A.A. Eddy, Use of Carbocyanine Dyes to Assay Membrane Potential of Mouse Ascites Tumor Cells. J.C. Freedman and T.S. Novak, Optical Measurement of Membrane Potential in Cells, Organelles, and Vesicles. O. Eidelman and Z.I. Cabantchik, Fluorescence Methods for Continuous Monitoring of Transport in Cells and Vesicles. D. Ammann and P. Caroni, Preparation and Use of Micro- and Macroelectrodes for Measurement of Transmembrane Potentials and Ion Activities. H. Garty and S.J.D. Karlish, Ion Channel-Mediated Fluxes in Membrane Vesicles: Selective Amplification of Isotope Uptake by Electrical Diffusion Potentials. J.R. Blinks, Use of Calcium-Regulated Photoproteins as Intracellular Ca2+ Indicators. A.V. Somlyo, H. Shuman, and A.P. Somlyo, Electron Probe X-ray Microanalysis of Ca2+, Mg2+, and Other Ions in Rapidly Frozen Cells. R. Tsien and T. Pozzan, Measurement of Cytosolic Free Ca2+ with Quin2.


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© 1989
Academic Press
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@from:Praise for the Series @qu:"The Methods in Enzymology series represents the gold-standard." @source:--NEUROSCIENCE @qu:"Incomparably useful." @source:--ANALYTICAL BIOCHEMISTRY @qu:"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." @source:--BIO/TECHNOLOGY @qu:"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." @source:--CHEMISTRY IN INDUSTRY @qu:"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." @source:--AMERICAN SOCIETY OF MICROBIOLOGY NEWS @qu:"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work." @source:--ENZYMOLOGIA @qu:"A series that has established itself as a definitive reference for biochemists." @source:--JOURNAL OF CHROMATOGRAPHY