The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today - truly an essential publication for researchers in all fields of life sciences.


Biochemists, cell biologists, microbiologists, pharmacologists, biomedical scientists, biophysicsts, analytical chemists, and physiologists.

Table of Contents

Transport Theory: G. Sachs and S. Fleischer, Transport Machinery: An Overview. M. Klingenberg, Survey of Carrier Methodology: Strategy for Identification, Isolation, and Characterization of Transport Systems. W.D. Stein, Kinetics of Transport: Analyzing, Testing, and Characterizing Models Using Kinetic Approaches. O.S. Andersen, Kinetics of Ion Movement Mediated by Carriers and Channels. R. Dev~aaes and R.M. Krupka, Inhibition Kinetics of Carrier Systems. J.V. Vadgama, Design of Simple Devices to Measure Solute Fluxes and Binding in Monolayer Cell Cultures. W.P. Jencks, Utilization of Binding Energy and Coupling Rules for Active Transport and Other Coupled Vectorial Processes. K.R. Runyan and R.B. Gunn, Generation of Steady-State Rate Equations for Enzyme and Carrier-Transport Mechanisms: A Microcomputer Program. Model Membranes and Their Characteristics: M.C. Woodle and D. Papahadjopoulos, Liposome Preparation and Size Characterization. T. Kondo, Preparation of Microcapsules from Human Erythrocytes: Use in Transport Experiments of Glutathione and Its S-Conjugate. H. Schindler, Planar Lipid-Protein Membranes: Strategies of Formation and of Detecting Dependencies of Ion Transport Functions on Membrane Conditions. D. Zakim and A.W. Scotto, Spontaneous Insertion of Integral Membrane Proteins into Preformed Unilamellar Vesicles. N.A. Dencher, Gentle and Fast Transmembrane Reconstitution of Membrane Proteins. R. Benz, H.-A. Kolb, P. L~adauger, and G. Stark, Ion Carriers in Planar Bilayers: Relaxation Techniques and Noise Analysis. D.W. Urry, T.L. Trapane, C.M. Venkatachalam, and R.B. McMichens, Ion In


No. of pages:
© 1989
Academic Press
Print ISBN:
Electronic ISBN:


@from:Praise for the Series @qu:"The Methods in Enzymology series represents the gold-standard." @source:--NEUROSCIENCE @qu:"Incomparably useful." @source:--ANALYTICAL BIOCHEMISTRY @qu:"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." @source:--BIO/TECHNOLOGY @qu:"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." @source:--CHEMISTRY IN INDUSTRY @qu:"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." @source:--AMERICAN SOCIETY OF MICROBIOLOGY NEWS @qu:"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work." @source:--ENZYMOLOGIA @qu:"A series that has established itself as a definitive reference for biochemists." @source:--JOURNAL OF CHROMATOGRAPHY