The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today - truly an essential publication for researchers in all fields of life sciences.


Biochemists, enzymologists, microbiologists, pharmacologists, biomedical researchers, biophysicists, analytical chemists, and physiologists.

Table of Contents

J.C. Skou, Overview: The Na,K-Pump. Preparation of Na+,K+-ATPase and Subunits. P.L. Jorgensen, Purification of Na+,K+-ATPase: Enzyme Sources, Preparative Problems, and Preparation from Mammalian Kidney. J.C. Skou and M. Esmann, Preparation of Membrane Na+,K+-ATPase from Rectal Glands of Squalus acanthias. T.W. Smith, Purification of Na+,K+-ATPase from the Supraorbital Salt Gland of the Duck. G.L. Peterson and L.E. Hokin, Preparation of Na+,K+-ATPase from Brine Shrimp. K.J. Sweadner, Preparation of the *ga(+) Isozyme of the Na+,K+-ATPase from Mammalian Axolemma. M. Esmann, Solubilization of Na+,K+-ATPase. E. Skriver, A.B. Maunsbach, H. Hebert, and P.L. Jorgensen, Crystallization of Membrane-Bound Na+,K+-ATPase in Two Dimensions. W.J. Ball, Jr., T.L. Kirley, and L.K. Lane, Preparation of Antibodies to Na+,K+-ATPase and Its Subunits. Assay of Na+,K+-ATPase Activity. M. Esmann, ATPase and Phosphatase Activity of Na+,K+-ATPase: Molar and Specific Activity, Protein Determination. J.G. Norby, Coupled Assay of Na+,K+-ATPase Activity. M.D. Resh, Identification and Quantitation of Na+,K+-ATPase by Back-Door Phosphorylation. Reconstitution of Na,K-Pump Activity. S.M. Goldin, M. Forgac, and G. Chin, Reconstitution of Na,K-Pump Activity by Cholate Dialysis: Sidedness and Stoichiometry. L.E. Hokin and J.F. Dixon, Reconstitution of the Na,K-Pump by Freeze*b1Thaw Sonication: Estimation of Coupling Ratio and Electrogenicity. F. Cornelius, Incorporation of C12E8-Solubilized Na+,K+-ATPase into Liposomes: Determination of Sidedness and Orientation. Analysis of the Pump Cycle. R. Blostein, Measurement of Na+ and K+ Transport and Na+,K+-ATPase Activity in Inside-Out Vesicles from Mammalian Erythrocytes. S.J.D. Karlish, Measurement of Active and Passive Na+ and K+ Fluxes in Reconstituted Vesicles. Measurement of Ligand Binding and Distance between Ligands. J.G. Norby and J. Jensen, Measurement of Binding of ATP and ADP to Na+,K+-ATPase. E.T. Wallick and A. Schwartz, Interaction of Cardiac


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© 1988
Academic Press
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@from:Praise for the Series @qu:"The Methods in Enzymology series represents the gold-standard." @source:--NEUROSCIENCE @qu:"Incomparably useful." @source:--ANALYTICAL BIOCHEMISTRY @qu:"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." @source:--BIO/TECHNOLOGY @qu:"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." @source:--CHEMISTRY IN INDUSTRY @qu:"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." @source:--AMERICAN SOCIETY OF MICROBIOLOGY NEWS @qu:"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work." @source:--ENZYMOLOGIA @qu:"A series that has established itself as a definitive reference for biochemists." @source:--JOURNAL OF CHROMATOGRAPHY