J.C. Skou, Overview: The Na,K-Pump. Preparation of Na+,K+-ATPase and Subunits. P.L. Jorgensen, Purification of Na+,K+-ATPase: Enzyme Sources, Preparative Problems, and Preparation from Mammalian Kidney. J.C. Skou and M. Esmann, Preparation of Membrane Na+,K+-ATPase from Rectal Glands of Squalus acanthias. T.W. Smith, Purification of Na+,K+-ATPase from the Supraorbital Salt Gland of the Duck. G.L. Peterson and L.E. Hokin, Preparation of Na+,K+-ATPase from Brine Shrimp. K.J. Sweadner, Preparation of the ga(+) Isozyme of the Na+,K+-ATPase from Mammalian Axolemma. M. Esmann, Solubilization of Na+,K+-ATPase. E. Skriver, A.B. Maunsbach, H. Hebert, and P.L. Jorgensen, Crystallization of Membrane-Bound Na+,K+-ATPase in Two Dimensions. W.J. Ball, Jr., T.L. Kirley, and L.K. Lane, Preparation of Antibodies to Na+,K+-ATPase and Its Subunits. Assay of Na+,K+-ATPase Activity. M. Esmann, ATPase and Phosphatase Activity of Na+,K+-ATPase: Molar and Specific Activity, Protein Determination. J.G. Norby, Coupled Assay of Na+,K+-ATPase Activity. M.D. Resh, Identification and Quantitation of Na+,K+-ATPase by Back-Door Phosphorylation. Reconstitution of Na,K-Pump Activity. S.M. Goldin, M. Forgac, and G. Chin, Reconstitution of Na,K-Pump Activity by Cholate Dialysis: Sidedness and Stoichiometry. L.E. Hokin and J.F. Dixon, Reconstitution of the Na,K-Pump by Freezeb1Thaw Sonication: Estimation of Coupling Ratio and Electrogenicity. F. Cornelius, Incorporation of C12E8-Solubilized Na+,K+-ATPase into Liposomes: Determination of Sidedness and Orientation. Analysis of the Pump Cycle. R. Blostein, Measurement of Na+ and K+ Transport and Na+,K+-ATPase Activity in Inside-Out Vesicles from Mammalian Erythrocytes. S.J.D. Karlish, Measurement of Active and Passive Na+ and K+ Fluxes in Reconstituted Vesicles. Measurement of Ligand Binding and Distance between Ligands. J.G. Norby and J. Jensen, Measurement of Binding of ATP and ADP to Na+,K+-ATPase. E.T. Wallick and A. Schwartz, Interaction of Cardiac
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Vanderbilt University, Nashville, Tennessee, U.S.A.
Vanderbilt University, Nashville, Tennesse, U.S.A.
California Institute of Technology, Division of Biology, Pasadena, U.S.A.
The Salk Institute, La Jolla, CA, USA