Biomedical Optical Phase Microscopy and Nanoscopy

Biomedical Optical Phase Microscopy and Nanoscopy

1st Edition - November 5, 2012

Write a review

  • Editors: Natan Shaked, Zeev Zalevsky, Lisa Satterwhite
  • eBook ISBN: 9780124158863
  • Hardcover ISBN: 9780124158719

Purchase options

Purchase options
DRM-free (Mobi, PDF, EPub)
Sales tax will be calculated at check-out

Institutional Subscription

Free Global Shipping
No minimum order


Written by leading optical phase microscopy experts, this book is a comprehensive reference to phase microscopy and nanoscopy techniques for biomedical applications, including differential interference contrast (DIC) microscopy, phase contrast microscopy, digital holographic microscopy, optical coherence tomography, tomographic phase microscopy, spectral-domain phase detection, and nanoparticle usage for phase nanoscopy The Editors show biomedical and optical engineers how to use phase microscopy for visualizing unstained specimens, and support the theoretical coverage with applied content and examples on designing systems and interpreting results in bio- and nanoscience applications.

Key Features

  • Provides a comprehensive overview of the principles and techniques of optical phase microscopy and nanoscopy with biomedical applications
  • Tips/advice on building systems and working with advanced imaging biomedical techniques, including interpretation of phase images, and techniques for quantitative analysis based on phase microscopy
  • Interdisciplinary approach that combines optical engineering, nanotechnology, biology and medical aspects of this topic. Each chapter includes practical implementations and worked examples


Biomedical engineers and researchers, Nanotechnology engineers and researchers, Optical engineers and researchers, Microscopy engineers

Table of Contents

  • Preface

    Part 1: Phase Contrast Microscopy and Differential Interference Contrast Microscopy

    Chapter 1. Phase Contrast Microscopy

    1.1 Introduction

    1.2 How Phase Contrast Works

    1.3 How to Acquire Phase Contrast Images

    1.4 Experimental Uses

    1.5 Limitations of Phase Optics


    Further Reading

    Chapter 2. Differential Interference Microscopy

    2.1 Introduction

    2.2 Measuring Shear Angle of DIC Prism

    2.3 Bias Optimization

    2.4 A Quantitative OI-DIC Microscope with Fast Modulation of Bias and Shear Direction

    2.5 Combination of OI-DIC and Orientation-Independent Polarization Imaging

    2.6 Combination of OI-DIC and Fluorescence Imaging



    Chapter 3. Long-Term Recordings of Live Human Cells Using Phase Contrast Microscopy

    3.1 Introduction

    3.2 The Microscope

    3.3 Correlative Live Cell–Fixed Cell Observations


    Further Reading

    Chapter 4. Phase Imaging in Plant Cells and Tissues

    4.1 Out of Focus Imaging

    4.2 Dark Field

    4.3 Phase Contrast Techniques

    4.4 Becke Line in Optical Microscopy

    4.5 DIC Microscopy

    4.6 Hoffmann Modulation Contrast

    4.7 Adaptive Optics Microscopy

    4.8 Interferometric Microscopy

    4.9 Second Harmonic Imaging Microscopy

    4.10 Magnetic Resonance Imaging of Plants Showing the Flow Component



    Part 2: Digital Holographic Phase Microscopy

    Chapter 5. Digital Holographic Microscopy for Measuring Biophysical Parameters of Living Cells

    5.1 Introduction

    5.2 Technical Introduction

    5.3 Biological Applications



    Chapter 6. Holographic Microscopy Techniques for Multifocus Phase Imaging of Living Cells

    6.1 Introduction

    6.2 DHM Setups for Live Cell Imaging

    6.3 Recording and Numerical Evaluation of Digital Holograms

    6.4 Refractive Index Determination of Cells in Suspension

    6.5 DHM Analysis of Morphology and Thickness Changes

    6.6 Quantitative Cell Division Monitoring

    6.7 Cell Tracking in 3D Environments

    6.8 Conclusions



    Chapter 7. Phase Unwrapping Problems in Digital Holographic Microscopy of Biological Cells

    7.1 Introduction

    7.2 Experimental Techniques

    7.3 Varying Reconstruction Distance Method

    7.4 Dual-Wavelength Phase Imaging

    7.5 Conclusions


    Chapter 8. On-Chip Holographic Microscopy and its Application for Automated Semen Analysis

    8.1 Introduction

    8.2 Partially Coherent In-Line Holography on a Chip

    8.3 Reconstruction of Microscopic Images and the Phase Recovery Process

    8.4 On-Chip Imaging of Sperm Using Partially Coherent Lensfree In-Line Holography

    8.5 High-Throughput On-Chip Semen Analysis Results

    8.6 Conclusions



    Chapter 9. Synthetic Aperture Lensless Digital Holographic Microscopy (SALDHM) for Superresolved Biological Imaging

    9.1 Introduction

    9.2 SALDHM Inside the Gabor’s Regime

    9.3 SALDHM Outside the Gabor’s Regime

    9.4 Conclusions


    Chapter 10. Combining Digital Holographic Microscopy with Microfluidics: A New Tool in Biotechnology

    10.1 Introduction

    10.2 Drive and Analyze

    10.3 Particle Tracking in 3D

    10.4 Conclusions


    Chapter 11. Holographic Motility Contrast Imaging of Live Tissues

    11.1 Introduction and Review

    11.2 Optical Coherence Imaging

    11.3 Motility Contrast Imaging

    11.4 Dynamic Light Scattering (DLS) Spectroscopy

    11.5 Tissue Dynamics Spectroscopy



    Part 3: Advanced Interferometric and Polarization Techniques

    Chapter 12. Tomographic Phase Microscopy (TPM)

    12.1 Introduction

    12.2 Theory—Optical Diffraction Tomography

    12.3 Experimental Implementation

    12.4 Video-Rate TPM

    12.5 Biological Applications

    12.6 Conclusion


    Chapter 13. Phase-Sensitive Optical Coherence Microscopy (OCM)

    13.1 Introduction

    13.2 OCM Principles

    13.3 Phase OCM

    13.4 Phase OCM Applications

    13.5 Conclusions


    Chapter 14. Polarization and Spectral Interferometric Techniques for Quantitative Phase Microscopy

    14.1 Introduction

    14.2 Dual-Interference Channel Quantitative Phase Microscopy

    14.3 Spectral-Domain Differential Interference Contrast Microscopy

    14.4 Spectral Multiplexing by RGB Color Channels

    14.5 Nonlinear Phase Dispersion Spectroscopy

    14.6 Conclusions



    Chapter 15. Polarization Microscopy

    15.1 Introduction

    15.2 Traditional Polarized Light Microscopy

    15.3 The Liquid-Crystal Polarization Microscope (LC-PolScope)

    15.4 Practical Considerations

    15.5 Polarized Light Imaging in Three Dimensions

    15.6 Conclusion

    15.7 Glossary of Polarization Optical Terms



    Part 4: Phase Nanoscopy

    Chapter 16. Is There a Fundamental Limit to Spatial Resolution in Phase Microscopy?

    16.1 Introduction

    16.2 Where Was Abbe’s Theory Incomplete?

    16.3 Parallel Full-Field Linear Imaging

    16.4 Reconstruction from Diffraction Patterns

    16.5 Structured Illumination Microscopy

    16.6 Imaging Three-Dimensional Phase Objects

    16.7 Conclusions



    Chapter 17. Nano-Holographic Interferometry for In-Vivo Observations

    17.1 Introduction: Theoretical Basis of Nano Fourier Transform Holography

    17.2 Gratings Illuminated by Evanescent Waves

    17.3 Basic Setup Utilized for the Observation of Nano-Objects

    17.4 System of Fringes Contained in the Recorded Image: Multi-k Vector Fields

    17.5 Determination of the Pitch of the Gratings

    17.6 Formation of the Holograms at the Nanoscale

    17.7 Procedures to Extract Metrological Information from the Recorded Images

    17.8 Analysis of the Scales

    17.9 Observation of Nano-Sized Objects

    17.10 Conclusions


    Chapter 18. Fluorescence Phase Microscopy (FPM) and Nanoscopy

    18.1 Introduction

    18.2 The Fluorescence Self-Interference Process

    18.3 Experimental Setups of FPM

    18.4 Applications of FPM

    18.5 Conclusions and Outlook



Product details

  • No. of pages: 432
  • Language: English
  • Copyright: © Academic Press 2012
  • Published: November 5, 2012
  • Imprint: Academic Press
  • eBook ISBN: 9780124158863
  • Hardcover ISBN: 9780124158719

About the Editors

Natan Shaked

Affiliations and Expertise

Senior Lecturer, Department of Biomedical Engineering, Tel Aviv University

Zeev Zalevsky

Affiliations and Expertise

Faculty of Engineering and the Center for Advanced Materials and Nanotechnology, Bar-Ilan University

Lisa Satterwhite

Affiliations and Expertise

Duke University, Durham

Ratings and Reviews

Write a review

There are currently no reviews for "Biomedical Optical Phase Microscopy and Nanoscopy"