Biomedical Optical Phase Microscopy and Nanoscopy

1st Edition

Print ISBN: 9780124158719
eBook ISBN: 9780124158863
Imprint: Academic Press
Published Date: 5th November 2012
Page Count: 432
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Written by leading optical phase microscopy experts, this book is a comprehensive reference to phase microscopy and nanoscopy techniques for biomedical applications, including differential interference contrast (DIC) microscopy, phase contrast microscopy, digital holographic microscopy, optical coherence tomography, tomographic phase microscopy, spectral-domain phase detection, and nanoparticle usage for phase nanoscopy

The Editors show biomedical and optical engineers how to use phase microscopy for visualizing unstained specimens, and support the theoretical coverage with applied content and examples on designing systems and interpreting results in bio- and nanoscience applications.

Key Features

  • Provides a comprehensive overview of the principles and techniques of optical phase microscopy and nanoscopy with biomedical applications.
  • Tips/advice on building systems and working with advanced imaging biomedical techniques, including  interpretation of phase images, and techniques for quantitative analysis based on phase microscopy.
  • Interdisciplinary approach that combines optical engineering, nanotechnology, biology and medical aspects of this topic. Each chapter includes practical implementations and worked examples.


Biomedical engineers and researchers
Nanotechnology engineers and researchers
Optical engineers and researchers

Microscopy engineers

Table of Contents


Part 1: Phase Contrast Microscopy and Differential Interference Contrast Microscopy

Chapter 1. Phase Contrast Microscopy

1.1 Introduction

1.2 How Phase Contrast Works

1.3 How to Acquire Phase Contrast Images

1.4 Experimental Uses

1.5 Limitations of Phase Optics


Further Reading

Chapter 2. Differential Interference Microscopy

2.1 Introduction

2.2 Measuring Shear Angle of DIC Prism

2.3 Bias Optimization

2.4 A Quantitative OI-DIC Microscope with Fast Modulation of Bias and Shear Direction

2.5 Combination of OI-DIC and Orientation-Independent Polarization Imaging

2.6 Combination of OI-DIC and Fluorescence Imaging



Chapter 3. Long-Term Recordings of Live Human Cells Using Phase Contrast Microscopy

3.1 Introduction

3.2 The Microscope

3.3 Correlative Live Cell–Fixed Cell Observations


Further Reading

Chapter 4. Phase Imaging in Plant Cells and Tissues

4.1 Out of Focus Imaging

4.2 Dark Field

4.3 Phase Contrast Techniques

4.4 Becke Line in Optical Microscopy

4.5 DIC Microscopy

4.6 Hoffmann Modulation Contrast

4.7 Adaptive Optics Microscopy

4.8 Interferometric Microscopy

4.9 Second Harmonic Imaging Microscopy

4.10 Magnetic Resonance Imaging of Plants Showing the Flow Component



Part 2: Digital Holographic Phase Microscopy

Chapter 5. Digital Holographic Microscopy for Measuring Biophysical Parameters of Living Cells

5.1 Introduction

5.2 Technical Introduction

5.3 Biological Applications



Chapter 6. Holographic Microscopy Techniques for Multifocus Phase Imaging of Living Cells

6.1 Introduction



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© Academic Press 2012
Academic Press
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"This interesting and inspiring book is a collection of articles on specific applications in optical imaging of cells and tissues…Phase imaging is understood in a broad sense and related techniques of super-resolution, polarization and optical coherence microscopy are described in a concise but very informative manner."--Optics & Photonics News Online, June 13, 2013

"This book has been structured to be self-contained and therefore useful for researchers and students at all levels who need a cutting edge review of techniques and applications, particularly biological and medical applications."--Reference and Research Book News, December 2012