Biomedical Electron Microscopy

Biomedical Electron Microscopy

Illustrated Methods and Interpretations

1st Edition - October 26, 1998

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  • Authors: Arvid Maunsbach, Björn Afzelius
  • eBook ISBN: 9780080528090
  • Hardcover ISBN: 9780124806108

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This comprehensive reference illustrates optimal preparation methods in biological electron microscopy compared with common methodological problems. Not only will the basic methodologies of transmission electron microscopy like fixation, microtomy, and microscopy be presented, but the authors also endeavor to illustrate more specialized techniques such as negative staining, autoradiography, cytochemistry, immunoelectron microscopy, and computer-assisted image analysis.

Key Features

  • Authored by the key leaders in the biological electron microscopy field
  • Illustrates both optimal and suboptimal or artifactual results in a variety of electron microscopy disciplines
  • Introduces students on how to read and interpret electron micrographs


Undergraduates, graduate students, and researchers in cell biology, developmental biology, microbiology, pathology, cancer research, structural biology, and endocrinology; research technicians

Table of Contents

  • Preface. Acknowledgments. Micrograph Interpretation. Fixatives. Fixative Vehicle. Fixative Application. Dehydration and Embedding. Freezing and Freeze-Substitution. Support Films. Ultramicrotomy. Section-Staining. Microscopy. Image Recording. Photographic and Digital Printing. Negative Staining. Autoradiography. Cytochemistry. Immunocytochemistry. Freeze-Fracturing and Shadowing. Sampling and Quantitation. Image Processing. 3D Reconstructions. Appendix: Practical Methods. Index. Acknowledgment for Figures.

Product details

  • No. of pages: 548
  • Language: English
  • Copyright: © Academic Press 1998
  • Published: October 26, 1998
  • Imprint: Academic Press
  • eBook ISBN: 9780080528090
  • Hardcover ISBN: 9780124806108

About the Authors

Arvid Maunsbach

Affiliations and Expertise

University of Aarhus; Aarhus, Denmark

Björn Afzelius

Affiliations and Expertise

Stockholm University, Stockholm, Sweden

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