Biomedical Electron Microscopy

1st Edition

Illustrated Methods and Interpretations

Authors: Arvid Maunsbach Björn Afzelius
Hardcover ISBN: 9780124806108
eBook ISBN: 9780080528090
Imprint: Academic Press
Published Date: 26th October 1998
Page Count: 548
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Description

This comprehensive reference illustrates optimal preparation methods in biological electron microscopy compared with common methodological problems. Not only will the basic methodologies of transmission electron microscopy like fixation, microtomy, and microscopy be presented, but the authors also endeavor to illustrate more specialized techniques such as negative staining, autoradiography, cytochemistry, immunoelectron microscopy, and computer-assisted image analysis.

Key Features

  • Authored by the key leaders in the biological electron microscopy field
  • Illustrates both optimal and suboptimal or artifactual results in a variety of electron microscopy disciplines
  • Introduces students on how to read and interpret electron micrographs

Readership

Undergraduates, graduate students, and researchers in cell biology, developmental biology, microbiology, pathology, cancer research, structural biology, and endocrinology; research technicians

Table of Contents

Preface. Acknowledgments. Micrograph Interpretation. Fixatives. Fixative Vehicle. Fixative Application. Dehydration and Embedding. Freezing and Freeze-Substitution. Support Films. Ultramicrotomy. Section-Staining. Microscopy. Image Recording. Photographic and Digital Printing. Negative Staining. Autoradiography. Cytochemistry. Immunocytochemistry. Freeze-Fracturing and Shadowing. Sampling and Quantitation. Image Processing. 3D Reconstructions. Appendix: Practical Methods. Index. Acknowledgment for Figures.

Details

No. of pages:
548
Language:
English
Copyright:
© Academic Press 1999
Published:
Imprint:
Academic Press
eBook ISBN:
9780080528090
Hardcover ISBN:
9780124806108

About the Author

Arvid Maunsbach

Affiliations and Expertise

University of Aarhus; Aarhus, Denmark

Björn Afzelius

Affiliations and Expertise

Stockholm University, Stockholm, Sweden

Reviews

"The book contains a wealth of information on the interpretation of electron micrographs - in a degree of detail that usually is known only on the work floor itself... This book has safeguarded important aspects of electron microscopy for future morphologists." --Trends in Cell Biology

"This is a superb book that should be in the hands of any new or experienced practitioner of electron microscopy. The writing is clear and direct and the micrographs set the highest standard of technique, quality, and usefulness. It will set a new standard of excellence in reinforcing the importance of the careful and appropriate use of electron microscopy in modern cell biology. No college, university, or life science library should be without it." --Doody's Publishing Reviews

"It must be the most comprehensive collection of artifacts to be published!...The strength of the book is in the clear and succinct presentation of techniques; the large number of micrographs illustrating the results; the numerous examples of artifacts and other defects; the high quality information given about specimen preparation for each example; and the references at the end of each chapter." --Micron

"This superbly illustrated collection will surely receive very heavy use..." --Ultramicroscopy, 1999

"The true value of this book is that it provides a known and high-quality reference standard against which one's own work may be judged and critically evaluated. It is of benefit to all those who work in the field of biological transmission electron microscopy, as well as the medical field indicated in the title..." --Proceedings of The Royal Microscopical Society, 1999