This volume covers cellulose and hemicellulose and includes proven and reproducible methods for research related to the conversion of carbohydrate polymers to usable monomeric units. Sections on the preparation of biomass materials and of substrates are included, as are sections on analytical methods and on the purification and assay of enzymes.


Biochemists; enzymologists; analytical, physical, and organic chemists; chemical engineers (fuels, energy, food processing); industrial and applied chemists; microbiologists.

Table of Contents

Cellulose. Preparation of Cellulosic Substrates: Increasing the Availability of Cellulose in Biomass Materials. Cadoxen Solvolysis of Cellulose. Preparation of Crystalline, Amorphous, and Dyed Cellulase Substrates. Preparation of Cellodextrins. Preparation of Cellodextrins: Another Perspective. Fluorogenic and Chromogenic Glycosides as Substrates and Ligands of Carbohydrases. Use of Complex Formation between Congo Red and Polysaccharides in Detection and Assay of Polysaccharide Hydrolases. Soluble, Dye-Labeled Polysaccharides for the Assay of Endohydrolases. Assays for Cellulolytic Enzymes: Methods for Measuring Cellulase Activities. Cellulase Assay Based on Cellobiose Dehydrogenase. Nephelometric and Turbidometric Assay for Cellulase. Selective Assay for Exo-1,4-/-Glucosidases. Viscosimetric Determination of Carboxymethylcellulase Activity. Staining Techniques for the Detection of the Individual Components of Cellulolytic Enzyme Systems. Chromatographic Methods for Carbohydrates: Liquid Chromatography of Carbohydrate Monomers and Oligomers. Quantitative Thin-Layer Chromatography of Sugars, Sugar Acids, and Polyalcohols. High-Performance Thin-Layer Chromatography of Starch, Cellulose, Xylan, and Chitin Hydrolyzates. Miscellaneous Methods for Cellulolytic Enzymes: Screening of Prokaryotes for Cellulose- and Hemicellulose-Degrading Enzymes. Chromatographic Separation of Cellulolytic Enzymes. Morphological Aspects of Wood Degradation. Purification of Cellulose-Degrading Enzymes: Cellulases of Pseudomonas fluorescens var. cellulosa. Cellulases of Cellulomonas uda. Cellulase of Ruminococcus albus. Cellulase of Trichoderma koningii. Cellulases of Trichoderma reesei.


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© 1988
Academic Press
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