Biology of Proteoglycans - 1st Edition - ISBN: 9780127506500, 9781483259406

Biology of Proteoglycans

1st Edition

Editors: Thomas N. Wight Robert P. Mecham
eBook ISBN: 9781483259406
Imprint: Academic Press
Published Date: 13th August 1987
Page Count: 414
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Biology of Proteoglycans provides a representative, but by no means inclusive, sample of current research on the role of proteoglycans in the cell biology of the extracellular matrix. The book is organized into four areas: methodological developments; proteoglycan metabolism; proteoglycans in cartilage; and proteoglycans in "soft" tissues. It begins with discussions of current methodological developments which have had enormous impact on understanding the complexity of proteoglycan structure. These include the use of monoclonal antibodies to probe the structure of the protein and carbohydrate portions of proteoglycans; and studies on the genes that code for the proteoglycan protein cores and associated proteins. This is followed by separate chapters on various aspects of proteoglycan metabolism, including the synthetic pathway used by cells to synthesize proteoglycans containing heparin and heparan sulfate; the molecular organization of different proteoglycans in cartilage; the role of proteoglycans and associated proteins in the calcification process in growth plate; and the emerging field of proteoglycans in "soft" or noncartilagenous tissue.

Table of Contents


Monoclonal Antibodies as Probes for Elucidating Proteoglycan Structure and Function

I. Introduction

II. Production, Screening, and Characterization of Monoclonal Antibodies Directed against Epitopes Present on Proteoglycan Substructures

III. Monoclonal Antibodies to Epitopes Present on Proteoglycan Monomers

IV. Monoclonal Antibodies to the Link Proteins of Cartilage Proteoglycan Aggregate

V. General Discussion


Molecular Biology of Proteoglycans and Link Proteins

I. Perspectives

IL Cell-Free Translation of mRNAs Encoding Proteoglycan Core Proteins and Link Proteins

III. Cloning of Proteoglycan Core Protein and Link Protein cDNAs

IV. Prospectives


Biosynthesis of Heparin and Heparan Sulfate

I. Introduction

II. The Proteoglycans—Structures and Interactions

III. Outline of the Biosynthetic Process

IV. Regulation of Polymer Modification

V. Organization of the Biosynthetic Apparatus

VI. Perspectives for the Future


Proteoglycan Metabolism by Rat Ovarian Granulosa Cells In Vitro

I. Introduction

II. Characterization of Granulosa Cell Proteoglycans

III. Metabolism of Granulosa Cell Proteoglycans

IV. General Considerations


Integral Membrane Proteoglycans as Matrix Receptors: Role in Cytoskeleton and Matrix Assembly at the Epithelial Cell Surface

I. Introduction

II. Characteristics of Mammary Epithelial Cell Surface Proteoglycan

III. Distribution of Epithelial Cell Surface Proteoglycan

IV. Heparan Sulfate Proteoglycan Binds Stromal Matrix Components

V. Cell Surface Proteoglycan as a Matrix Anchor to the Cytoskeleton

VI. Cell Surface Proteoglycan is a Matrix Receptor

VII. Summary


Structural Organization of Proteoglycans in Cartilage

I. Introduction

II. Macromolecular Structure of Isolated Proteoglycans Based on Electron-Microscopic Analysis

III. Preservation Methods for Proteoglycans in Tissue

IV. Morphology of Proteoglycans in Cartilage

V. Spatial Arrangement of Proteoglycans in Native Tissue


Proteoglycans, Chondrocalcin, and the Calcification of Cartilage Matrix in Endochondrial Ossification

I. Anatomical Organization of the Growth Plate

II. Composition and Organization of the Cartilage Matrix of Growth Plate

III. Changes in the Proteoglycans of the Growth Plate Associated with Calcification

IV. Purified Proteoglycans and Glycosaminoglycans Can Bind Calcium and Inhibit Calcification In Vitro

V. A Unifying Hypothesis to Explain the Proteoglycan Changes That May Occur in Calcifying Growth Plate Cartilage


Biochemical Basis of Age-Related Changes in Proteoglycans

I. Introduction

II. Aging and Maturation

III. Proteoglycan Populations: Age-Related Changes

IV. Biosynthesis

V. Proteoglycans in Disease


Extracellular Matrix Components of the Synapse

I. Introduction

II. ECM Components are Involved in Synaptic Regeneration

III. Axonal Transport of Sulfated Materials

IV. Proteoglycans in Secretory Vesicles

V. A Synaptic Junctional Proteoglycan Related to the Vesicle Form

VI. Purification and Characterization of the ECM Proteoglycan

VII. The Antigenic Determinant of Electric Organ TAP

VIII. Insertion of the Anchorage Protein

IX. Other Components of the ECM at the Synapse


Blood Vessel Proteoglycans

I. Introduction

II. Biochemistry of Arterial Proteoglycans

III. Morphology of Arterial Proteoglycans

IV. Proteoglycans in Atherosclerosis

V. Cell Culture Studies

VI. Concluding Remarks


Heparan Sulfate Species and Blood Vessel Wall Function

I. Introduction

II. The Role of Heparan Sulfate Species as a Regulator of Coagulation Mechanism Activity

III. Role of Heparan Sulfate as a Modulator of Smooth Muscle Proliferation


Cell-Associated Proteoglycans in Human Malignant Melanoma

I. Extracellular Matrix Composition during Tumorigenesis

II. Chondroitin Sulfate Proteoglycans Synthesized by Human Melanoma Cells: Production of Monoclonal Antibodies

III. Biosynthesis and Intracellular Transport of Proteoglycans

IV. Conclusion


Intracellular Proteoglycans in Cells of the Immune System

I. Introduction

II. Localization and Characterization of Secretory Granule Proteoglycans in Different Cell Types

III. Speculated Functions of Intracellular Proteoglycans in Immunity and Inflammation

IV. Summary




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© Academic Press 1987
Academic Press
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About the Editor

Thomas N. Wight

Robert P. Mecham

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