Biology of Proteoglycans

Biology of Proteoglycans

1st Edition - August 13, 1987

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  • Editors: Thomas N. Wight, Robert P. Mecham
  • eBook ISBN: 9781483259406

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Biology of Proteoglycans provides a representative, but by no means inclusive, sample of current research on the role of proteoglycans in the cell biology of the extracellular matrix. The book is organized into four areas: methodological developments; proteoglycan metabolism; proteoglycans in cartilage; and proteoglycans in "soft" tissues. It begins with discussions of current methodological developments which have had enormous impact on understanding the complexity of proteoglycan structure. These include the use of monoclonal antibodies to probe the structure of the protein and carbohydrate portions of proteoglycans; and studies on the genes that code for the proteoglycan protein cores and associated proteins. This is followed by separate chapters on various aspects of proteoglycan metabolism, including the synthetic pathway used by cells to synthesize proteoglycans containing heparin and heparan sulfate; the molecular organization of different proteoglycans in cartilage; the role of proteoglycans and associated proteins in the calcification process in growth plate; and the emerging field of proteoglycans in "soft" or noncartilagenous tissue.

Table of Contents

  • Preface

    Monoclonal Antibodies as Probes for Elucidating Proteoglycan Structure and Function

    I. Introduction

    II. Production, Screening, and Characterization of Monoclonal Antibodies Directed against Epitopes Present on Proteoglycan Substructures

    III. Monoclonal Antibodies to Epitopes Present on Proteoglycan Monomers

    IV. Monoclonal Antibodies to the Link Proteins of Cartilage Proteoglycan Aggregate

    V. General Discussion


    Molecular Biology of Proteoglycans and Link Proteins

    I. Perspectives

    IL Cell-Free Translation of mRNAs Encoding Proteoglycan Core Proteins and Link Proteins

    III. Cloning of Proteoglycan Core Protein and Link Protein cDNAs

    IV. Prospectives


    Biosynthesis of Heparin and Heparan Sulfate

    I. Introduction

    II. The Proteoglycans—Structures and Interactions

    III. Outline of the Biosynthetic Process

    IV. Regulation of Polymer Modification

    V. Organization of the Biosynthetic Apparatus

    VI. Perspectives for the Future


    Proteoglycan Metabolism by Rat Ovarian Granulosa Cells In Vitro

    I. Introduction

    II. Characterization of Granulosa Cell Proteoglycans

    III. Metabolism of Granulosa Cell Proteoglycans

    IV. General Considerations


    Integral Membrane Proteoglycans as Matrix Receptors: Role in Cytoskeleton and Matrix Assembly at the Epithelial Cell Surface

    I. Introduction

    II. Characteristics of Mammary Epithelial Cell Surface Proteoglycan

    III. Distribution of Epithelial Cell Surface Proteoglycan

    IV. Heparan Sulfate Proteoglycan Binds Stromal Matrix Components

    V. Cell Surface Proteoglycan as a Matrix Anchor to the Cytoskeleton

    VI. Cell Surface Proteoglycan is a Matrix Receptor

    VII. Summary


    Structural Organization of Proteoglycans in Cartilage

    I. Introduction

    II. Macromolecular Structure of Isolated Proteoglycans Based on Electron-Microscopic Analysis

    III. Preservation Methods for Proteoglycans in Tissue

    IV. Morphology of Proteoglycans in Cartilage

    V. Spatial Arrangement of Proteoglycans in Native Tissue


    Proteoglycans, Chondrocalcin, and the Calcification of Cartilage Matrix in Endochondrial Ossification

    I. Anatomical Organization of the Growth Plate

    II. Composition and Organization of the Cartilage Matrix of Growth Plate

    III. Changes in the Proteoglycans of the Growth Plate Associated with Calcification

    IV. Purified Proteoglycans and Glycosaminoglycans Can Bind Calcium and Inhibit Calcification In Vitro

    V. A Unifying Hypothesis to Explain the Proteoglycan Changes That May Occur in Calcifying Growth Plate Cartilage


    Biochemical Basis of Age-Related Changes in Proteoglycans

    I. Introduction

    II. Aging and Maturation

    III. Proteoglycan Populations: Age-Related Changes

    IV. Biosynthesis

    V. Proteoglycans in Disease


    Extracellular Matrix Components of the Synapse

    I. Introduction

    II. ECM Components are Involved in Synaptic Regeneration

    III. Axonal Transport of Sulfated Materials

    IV. Proteoglycans in Secretory Vesicles

    V. A Synaptic Junctional Proteoglycan Related to the Vesicle Form

    VI. Purification and Characterization of the ECM Proteoglycan

    VII. The Antigenic Determinant of Electric Organ TAP

    VIII. Insertion of the Anchorage Protein

    IX. Other Components of the ECM at the Synapse


    Blood Vessel Proteoglycans

    I. Introduction

    II. Biochemistry of Arterial Proteoglycans

    III. Morphology of Arterial Proteoglycans

    IV. Proteoglycans in Atherosclerosis

    V. Cell Culture Studies

    VI. Concluding Remarks


    Heparan Sulfate Species and Blood Vessel Wall Function

    I. Introduction

    II. The Role of Heparan Sulfate Species as a Regulator of Coagulation Mechanism Activity

    III. Role of Heparan Sulfate as a Modulator of Smooth Muscle Proliferation


    Cell-Associated Proteoglycans in Human Malignant Melanoma

    I. Extracellular Matrix Composition during Tumorigenesis

    II. Chondroitin Sulfate Proteoglycans Synthesized by Human Melanoma Cells: Production of Monoclonal Antibodies

    III. Biosynthesis and Intracellular Transport of Proteoglycans

    IV. Conclusion


    Intracellular Proteoglycans in Cells of the Immune System

    I. Introduction

    II. Localization and Characterization of Secretory Granule Proteoglycans in Different Cell Types

    III. Speculated Functions of Intracellular Proteoglycans in Immunity and Inflammation

    IV. Summary



Product details

  • No. of pages: 414
  • Language: English
  • Copyright: © Academic Press 1987
  • Published: August 13, 1987
  • Imprint: Academic Press
  • eBook ISBN: 9781483259406

About the Editors

Thomas N. Wight

Robert P. Mecham

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