- Print ISBN 9780444535580
- Electronic ISBN 9780444535597
IR spectroscopy has become without any doubt a key technique to answer questions raised when studying the interaction of proteins or peptides with solid surfaces for a fundamental point of view as well as for technological applications.
Principle, experimental set ups, parameters and interpretation rules of several advanced IR-based techniques; application to biointerface characterisation through the presentation of recent examples, will be given in this book. It will describe how to characterise amino acids, protein or bacterial strain interactions with metal and oxide surfaces, by using infrared spectroscopy, in vacuum, in the air or in an aqueous medium. Results will highlight the performances and perspectives of the technique.
Chapter 1. RAIRS under ultrahigh vacuum conditions on metal surfaces
Chapter 2. PM-IRRAS at liquid interfaces
Chapter 3. Infrared spectroscopy for characterization of biomolecular interfaces
Chapter 4. Infrared analysis of biomolecule attachment to functionalized silicon surfaces
Chapter 5. Attenuated total reflection infrared (ATR-IR) spectroscopy, modulation excitation spectroscopy (MES), and vibrational circular dichroism (VCD)
Chapter 6. Synchrotron infrared interface science
Chapter 7. IR spectroscopy for biorecognition and molecular sensing
Chapter 8. Advanced infrared glasses for biochemical sensing
Chapter 9. AFM-IR: photothermal infrared nanospectroscopy
Chapter 10. Sum-frequency generation spectroscopy of biointerfaces