This book provides in-depth information on basic and applied aspects of biohydrogen production. It begins with an introduction to the topic, and follows with the basic scientific aspects of biohydrogen production, such as the enzyme involved in biohydrogen production, the microorganisms and metabolic engineering information. It then provides state-of-art information on various aspects of biohydrogen production methods such as from solid wastes, from industrial effluents, thermo-chemical route for biohydrogen production, etc. It also includes information on engineering aspects such as the design of bioreactors for biohydrogen production and scale-up issues. Finally, it touches on the issues of hydrogen economy and commercialization. The book introduces you to all aspects of biohydrogen research, helping you understand the various issues involved and plan your own research based on recent findings and commercial needs.

Key Features

  • Provides information on the most advanced and innovative biohydrogen technologies, including fermentation and metabolic processes
  • Provides examples on large-scale and commercial applications of biohydrogen processes and explains the steps necessary for scaling-up
  • Explains the chemistry/theory of the processes involved and provides information on integration of the various processes and technologies on biohydrogen
  • Guides through the process design, reactors and materials selection
  • Devotes a whole chapter on the economical aspects of the processes and their commercialization


Chemists and chemical engineers, biochemical engineers, Biotechnologists, bioengineers, , environmental engineers, academics and research students in these areas as well researchers in government and corporate research labs

Table of Contents

Foreword (Michael Seibert)
Preface (Ashok Pandey, Jo-Shu Chang, Patrick C. Hallenbeck, Christian Larroche)
1 Biohydrogen Production: An Introduction (S. Venkata Mohan, Ashok Pandey)
2 Fundamentals of Biohydrogen (Patrick C. Hallenbeck)
3 Metabolic Engineering of Microorganisms for Biohydrogen Production (You-Kwan Oh, Subramanian Mohan Raj, Gyoo Yeol Jung, Sunghoon Park)
4 Insurmountable Hurdles for Fermentative H2 Production? (Patrik R. Jones, M. Kalim Akhtar)
5 Hydrogenase (Philippe Constant, Patrick C. Hallenbeck)
6 Biohydrogen Production from Organic Wastes by Dark Fermentation (G. Balachandar, Namita Khanna, Debabrata Das)
7 Photofermentative Biohydrogen Production (Patrick C. Hallenbeck)
8 Biohydrogen Production from Algae (Man Kee Lam, Keat Teong Lee)
9 Biohydrogen from Renewable Resources (Ganesh D. Saratale, Rijuta G. Saratale, Jo-Shu Chang)
10 Biohydrogen Production from Wastewater (S. Venkata Mohan, K. Chandrasekhar, P. Chiranjeevi, P. Suresh Babu)
11 Fermentative Biohydrogen Production from Solid Wastes (Mi-Sun Kim, Jaehwan Cha, Dong-Hoon Kim)
12 Thermochemical Route for Biohydrogen Production (Thallada Bhaskar, Bhavya Balagurumurthy, Rawel Singh, Mukesh Kumar Poddar)
13 Bioreactor and Bioprocess Design for Biohydrogen Production (Kuan-Yeow Show, Duu-Jong Lee)
14 Scale-up and Commercial Applications of Biohydrogen Production Processes (Biswarup Sen, Chen-Yeon Chu, Chiu-Yue Lin)


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About the editors

Ashok Pandey

Professor Ashok Pandey is Deputy Director CSIR’s National Institute for Interdisciplinary Science and Technology at Trivandrum and heading the Centre for Biofuels and Biotechnology Division there. Professor Pandey’s research interests are on bio-based economy (biomass-based biorefinery) for the production of fuels and chemicals. He has >1000 publications/communications, which include 14 patents & design copyright, 34 books, 99 book chapters, 391 original and review papers, etc with h index of 65 and >17,700 citation (Goggle scholar). Professor Pandey is the recipient of many national and international awards and fellowships, which include Fellow of International Society for Energy, Environment and Sustainability, National Academy of Science (India), Biotech Research Society, India, International Organization of Biotechnology and Bioengineering, and Association of Microbiologists of India; Honorary Doctorate degree from Univesite Blaise Pascal, France; Thomson Scientific India Citation Laureate Award, USA; Lupin Visiting Fellowship, Visiting Professor in the University Blaise Pascal, France, Federal University of Parana, Brazil and EPFL, Switzerland; Best Scientific Work Achievement award, Govt of Cuba; UNESCO Professor; Raman Research Fellowship Award, CSIR; GBF, Germany and CNRS, France Fellowship; Young Scientist Award, etc. He was Chairman of the International Society of Food, Agriculture and Environment, Finland ((Food & Health) during 2003-2004. He is Founder President of the Biotech Research Society, India (; International Coordinator & General Secretary of International Forum on Industrial Bioprocesses, France ( and Vice-President of the International Society for Energy, Environment & Sustainability ( and All India Biotech Association ( Prof Pandey is Editor-in-chief of Bioresource Technology, Honorary Executive Advisors of Journal of Water Sustainability and Journal of Energy and Env

Patrick Hallenbeck

Patrick C. Hallenbeck, microbiologist, obtained a Ph.D. in Biophysics from the University of California Berkeley (USA) and held post-doctoral positions at the French Nuclear Energy Center, Grenoble (France), and the University of California, Davis (USA) before taking up a position as professor in the Department of Microbiology and Immunology, University of Montreal. Dr. Hallenbeck’s research interests are in microbial physiology, applied microbiology and biotechnology, biological energy production, anaerobic metabolism, and nitrogen fixation. Dr. Hallenbeck is the Canadian representative to Task 21 (Biohydrogen) of the Hydrogen Implementing Agreement of the IEA and President of the Biohydrogen subdivision of the International Hydrogen Association. He has served on the organizing committees of numerous international conferences, was chair of ISPP2009, and is chair of BioH2 2013. He is author of over fifty original research publications, twenty-two reviews and book chapters, and numerous abstracts in conference proceedings. He is editor of two books. Prof. Hallenbeck won Outstanding Scientist award for 2012 from the IFIBiop ( He is currently researching the role of metabolite sensing and signalling in the regulation of microbial metabolism, and using metabolic engineering to increase biofuels production. He is editorial board member in Bioresource Technology, Frontiers in Microbiology, and Marine Biotechnology.


"Editor Pandey…unites international editors and contributors to report on biohydrogen production at the molecular and metabolic level and to describe related engineering aspects, waste utilization, and large-scale commercial applications…The book will be useful for researchers and practitioners in biohydrogen, microbial bioprocesses, biofuels, and industrial waste.", February 2014

"Editor Pandey…unites international editors and contributors to report on biohydrogen production at the molecular and metabolic level and to describe related engineering aspects, waste utilization, and large-scale commercial applications…the book will be useful for researchers and practitioners in biohydrogen, microbial bioprocesses, biofuels, and industrial waste." --Reference & Research Book News, December 2013