Hydrogen is an almost ideal fuel and its wider use will result in an improvement in the environment due to factors including decreased air pollution. Hydrogen is the element of greatest abundance in the universe; however, its production from renewable resources remains a major challenge.
The papers presented within this volume enhance and expand upon presentations made at the "Workshop on Biohydrogen 99", Tsukuba, Japan. The contents evaluate the current status of Biohydrogen research worldwide and consider future research directions.
Contributions from leading international experts cover the breadth of Biohydrogen R and D, from production to genetic engineering and molecular biology. This volume is designed to be an invaluable resource for researchers and other professionals who wish to obtain an overview of Biohydrogen R and D.


It is expected that this volume will prove useful both to researchers as well as interested persons who wish to obtain an overview of Biohydrogen research and development.

Table of Contents

1. Hydrogen Production. Hydrogen production by photosynthetic bacteria: culture media, yield and efficiencies (J.S. Rocha, M.J. Barbosa, R.H. Wijffels). Hydrogen and Poly- (hydroxy) alkanoate production from organic aids by photosynthetic bacteria (N. Noparatnaraporn, K. Takeno, K. Sasaki). Hydrogen from Biomass (T. Wakayama, J. Miyake). Hydrogen photoproduction from starch in algal biomass (A. Ike, H. Kawaguchi, K. Hirata, K. Miyamoto). Photobiological hydrogen production and nitrogenase activity in some heterocystous cyanobacteria (H. Masukawa, K. Nakamura, M. Mochimaru, H. Sakurai). Strategies for improving oxygen tolerance of algal hydrogen production (M. Seibert, T. Flynn, M.L. Ghirardi). 2. Hydrogen World. Emerging hydrogen energy systems and biology (T. Ohta). Biohydrogen: an opportunity for international cooperation (A. San Pietro, J.R. Benemann). Hydrogenotrophy - a new aspect of biohydrogen (Y. Igarashi). 3. Biochemical and Metabolic Systems. Maximising photosynthetic efficiencies and hydrogen production in microalga cultures (J. Polle, S. Kanakagiri, J.R. Benemann, A. Melis). A scheme for developing the yield of hydrogen by fermentation (S. Tanisho). 4. Molecular Biology. Cyanobacterial hydrogenases and biohydrogen: present status and future potential (P. Lindblad, P. Tamagnini). Integration of hydrogen evolving systems with cellular metabolism: the molecular biology and biochemistry of electron transport factors and associated reductases (P.C. Hallenbeck). 5. Genetic Engineering. Screening of marine photosynthetic microorganisms and hydrogen production (T. Matsunaga, H. Takeyama). Metabolic engineering approaches for the improvement of bacterial hydrogen production based on Escherichia coli mixed acid fe


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About the authors

J. Miyake

Affiliations and Expertise

National Institute for Advanced Interdisciplinary Research, AIST/MITI, Tsukuba, Ibaraki, Japan

T. Matsunaga

Affiliations and Expertise

Tokyo University of Agriculture and Technology, Koganei, Tokyo, Japan

A. San Pietro

Affiliations and Expertise

Indiana University, Bloomington, Indiana, USA