Bioconjugate Techniques - 2nd Edition - ISBN: 9780123705013, 9780080568720

Bioconjugate Techniques

2nd Edition

Authors: Greg Hermanson
eBook ISBN: 9780080568720
Hardcover ISBN: 9780123705013
Imprint: Academic Press
Published Date: 18th April 2008
Page Count: 1323
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Description

Bioconjugate Techniques, 2nd Edition, is the essential guide to the modification and cross linking of biomolecules for use in research, diagnostics, and therapeutics. It provides highly detailed information on the chemistry, reagent systems, and practical applications for creating labeled or conjugate molecules. It also describes dozens of reactions with details on hundreds of commercially available reagents and the use of these reagents for modifying or cross linking peptides and proteins, sugars and polysaccharides, nucleic acids and oligonucleotides, lipids, and synthetic polymers.

Key Features

  • A one-stop source for proven methods and protocols for synthesizing bioconjugates in the lab
  • Step-by-step presentation makes the book an ideal source for researchers who are less familiar with the synthesis of bioconjugates
  • More than 600 figures that visually describe the complex reactions associated with the synthesis of bioconjugates
  • Includes entirely new chapters on the latest areas in the field of bioconjugation as follows:
    Microparticles and nanoparticles
    Silane coupling agents
    Dendrimers and dendrons
    Chemoselective ligation
    Quantum dots
    Lanthanide chelates
    Cyanine dyes
    Discrete PEG compounds
    Buckyballs,fullerenes, and carbon nanotubes
    Mass tags and isotope tags
    Bioconjugation in the study of protein interactions

Readership

Researchers in biochemistry, molecular biology, immunology, pharmacology, cell biology, and microbiology

Table of Contents

PART I Bioconjugate Chemistry
1. Functional Targets
2. The Chemistry of Reactive Groups

PART II Bioconjugate Reagents
3. Zero-Length Cross-Linkers
4. Homobifunctional Cross-linkers
5. Heterobifunctional Cross-linkers
6. Trifunctional Cross-linkers
7. Dendrimers and Dendrons
8. Cleavable Reagent Systems
9. Fluorescent Probes
10. Bifunctional Chelating Agents and Radioimmunoconjugates
11. Biotinylation Reagents
12. Iodination Reagents
13. Silane Coupling Agents
14. Microparticles and Nanoparticles
15. Buckyballs, Fullerenes, and Carbon Nanotubes
16. Mass Tags and Isotope Tags
17. Chemoselective Ligation; Bioorthogonal Reagents
18. Discrete PEG Compounds

PART III Bioconjugate Applications
19. Preparation of Hapten - Carrier Immunogen Conjugates
20. Antibody Modification and Conjugation
21. Immunotoxin Conjugation Techniques
22. Preparation of Liposome Conjugates and Derivatives
23. Avidin - Biotin Systems
24. Preparation of Colloidal-Gold-Labeled Proteins
25. Modification with Synthetic Polymers
26. Enzyme Modification and Conjugation
27. Nucleic Acid and Oligonucleotide Modification and Conjugation
28. Bioconjugation for the Study of Protein Interactions

Details

No. of pages:
1323
Language:
English
Copyright:
© Academic Press 2008
Published:
Imprint:
Academic Press
eBook ISBN:
9780080568720
Hardcover ISBN:
9780123705013

About the Author

Greg Hermanson

Affiliations and Expertise

Director of Technology, Thermo Fisher Scientific, Rockford, IL USA

Ratings and Reviews