Biochemical Spectroscopy, Volume 246

1st Edition

Print ISBN: 9780121821470
eBook ISBN: 9780080883595
Imprint: Academic Press
Published Date: 16th February 1995
Page Count: 816


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This volume includes spectroscopic methods for the characterization of macromolecules. Methods span the electromagnetic spectrum from X-ray to microwaves. It focuses on the types of information that can be derived: how measurements are made; state of the art apparatus; data acquisition, analysis, and interpretation; casebook examples; and new developments and future directions.

Key Features

@introbul:Key Features @bul: Ultraviolet and Visible Spectroscopy, including @subbul: Absorption and Circular Dichroism

  • Transient Absorption and Kinetics
  • Linear Dichroism and Fluorescence
  • Vibrational Spectroscopy
  • Magnetic Resonance Spectroscopy
  • X-Ray Spectroscopy


Biochemists, biophysicists, pharmaceutical chemists, biomedical researchers, and graduate students.

Table of Contents

K. Sauer, Why Spectroscopy? Which Spectroscopy?

Ultraviolet/Visible Spectroscopy:

I. Tinoco, Jr., Optical Spectroscopy: General Principles and Overview.

A. Absorption and Circular Dichroism:

D.M. Gray, S.-H. Hung, and K.H. Johnson, Absorption and Circular Dichroism Spectroscopy of Nucleic Acid Duplexes and Triplexes.

R.W. Woody, Circular Dichroism.

E.I. Solomon, M.L. Kirk, D.R. Gamelin, and S. Pulver, Bioinorganic Spectroscopy.

J.C.Sutherland, Magnetic Circular Dichroism.

R.H. Austin and S. Erramilli, Low-Temperature Spectroscopy.

B. Transient Absorption and Kinetics:

P.S. Brzovic and M.F. Dunn, Rapid-Scanning Ultraviolet/Visible Spectroscopy Applied in Stopped-Flow Studies.

H. van Amerongen and R. van Grondelle, Transient Absorption Spectroscopy in Study of Processes and Dynamics in Biology.

J. Friedrich, Hole Burning Spectroscopy and Physics of Proteins.

C. Linear Dichroism and Fluorescence:

H. van Amerongen and W.S. Struve, Polarized Optical Spectroscopy of Chromoproteins.

D.M. Jameson and W.H. Sawyer, Fluorescence Anisotropy Applied to Biomolecular Interactions.

P.R. Selvin, Fluorescence Resonance Energy Transfer.

A.R. Holzwarth, Time-Resolved Fluorescence Spectroscopy.

A. Waggoner, Covalent Labeling of Proteins and Nucleic Acids with Fluorophores.

Vibrational Spectroscopy:

R.A. Mathies, Biomolecular Vibrational Spectroscopy.

W.L.Peticolas, Raman Spectroscopy of DNA and Proteins.

T.G. Spiro and R.S. Czernuszewicz, Resonance Raman Spectrosc


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© Academic Press 1995
Academic Press
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