Biochemical Applications of Raman and Resonance Raman Spectroscopes - 1st Edition - ISBN: 9780121596507, 9780323158121

Biochemical Applications of Raman and Resonance Raman Spectroscopes

1st Edition

Authors: P Carey
eBook ISBN: 9780323158121
Imprint: Academic Press
Published Date: 28th July 1982
Page Count: 276
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Biochemical Applications of Raman and Resonance Raman Spectroscopies focuses on the application of Raman and resonance Raman spectroscopies to biochemical problems. The book reviews biological systems and details the application of Raman spectroscopy to biological molecules such as proteins, nucleic acids, and lipids. It also looks at codevelopments of lasers, optics, and electronics that drive advances in experimental Raman spectroscopy, along with the important ramifications of these advances for biochemical applications. This volume is organized into eight chapters and begins with an overview of the theoretical and experimental aspects of Raman spectroscopy, including a very brief explanation of what Raman and resonance Raman spectroscopies are and a discussion of their advantages and disadvantages for biochemical studies. The explanation of the Raman and resonance Raman effects is taken up in more detail in the next chapter, which develops the concept of the vibrational motions of molecules by initially considering mechanical ""ball and spring"" models and goes on to use this concept to formulate a classical model for Raman scattering. The resonance Raman effect is then described by another model which emphasizes the discrete or quantized energy levels available to a molecule. The reader is also introduced to the experimental aspects of Raman spectroscopy and the application of Raman spectroscopy across the entire field of biochemistry. Each chapter contains an outline of the basic chemistry and biochemical nomenclature involved. This book will be of interest to chemists, biochemists, and spectroscopists, as well as graduate students and experienced research workers.

Table of Contents


Chapter l. Introduction

I. Raman Scattering

II. The Appearance of a Raman Spectrum

III. Basic Units

IV. Raman, Resonance Raman, and Infrared Spectroscopies

V. Advantages and Disadvantages of Raman Spectroscopy for Biochemical Studies

Chapter 2. Principles of Raman Spectroscopy

I. Introduction

II. The Vibrations of a Diatomic Molecule from a Classical Standpoint

III. A Classical Model for Raman Scattering

IV. Comparison with Infrared Spectroscopy

V. A Quantum Mechanical Picture of Raman Scattering

VI. The Vibrations of a Polyatomic Molecule

VII. Polarization Properties of Raman Scattering

VIII. Raman Intensities

Chapter 3. Experimental Raman Spectroscopy

I. Basic Optics of the Raman Experiment

II. Sampling Techniques and Sample Problems

III. Sophisticated Techniques

Chapter 4. Protein Conformation from Raman and Resonance Raman Spectra

I. Protein Structure

II. Introduction to Raman and Resonance Raman Studies of Proteins

III. Raman Studies of Proteins

IV. Resonance Raman Studies of the Chromophores of the Polypeptide Chain Using Excitation below 300 nm

Chapter 5. Resonance Raman Studies of Natural, Protein-Bound Chromophores

I. Introduction

II. Heme Proteins

III. Vitamin B12 (Cobalamin)

IV. Chlorophylls

V. Carotenoids

VI. The Visual Pigments and Bacteriorhodopsin

VII. Flavin Nucleotides

VIII. Metalloproteins

Chapter 6. Resonance Raman Labels

I. Introduction

II. Drug-Protein Complexes and Other Enzyme-Inhibitor Systems

III. Antibody-Hapten Complexes

IV. Permanently Labeled Sites—Arsanilazocarboxypeptidase A

V. Enzyme-Substrate Reactions

VI. Nucleic Acids and Membranes

Chapter 7. Nucleic Acids and Nucleic Acid-Protein Complexes

I. The Structure of Nucleic Acids

II. Polynucleotide Structure from the Normal Raman Spectrum

III. Applications to RNA and DNA Structures

IV. Viruses and Other Nucleic Acid-Protein Complexes

V. Resonance Raman Studies of Nucleic Acids

Chapter 8. Lipids, Membranes, and Carbohydrates

I. Structures of Lipids and Membranes

II. Raman Spectra of Lipids and Membranes

III. Carbohydrates

Suggestions for Further Reading




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© Academic Press 1982
Academic Press
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About the Author

P Carey

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