Biochemical Applications of Raman and Resonance Raman Spectroscopes

Biochemical Applications of Raman and Resonance Raman Spectroscopes

1st Edition - July 28, 1982

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  • Author: P Carey
  • eBook ISBN: 9780323158121

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Biochemical Applications of Raman and Resonance Raman Spectroscopies focuses on the application of Raman and resonance Raman spectroscopies to biochemical problems. The book reviews biological systems and details the application of Raman spectroscopy to biological molecules such as proteins, nucleic acids, and lipids. It also looks at codevelopments of lasers, optics, and electronics that drive advances in experimental Raman spectroscopy, along with the important ramifications of these advances for biochemical applications. This volume is organized into eight chapters and begins with an overview of the theoretical and experimental aspects of Raman spectroscopy, including a very brief explanation of what Raman and resonance Raman spectroscopies are and a discussion of their advantages and disadvantages for biochemical studies. The explanation of the Raman and resonance Raman effects is taken up in more detail in the next chapter, which develops the concept of the vibrational motions of molecules by initially considering mechanical ""ball and spring"" models and goes on to use this concept to formulate a classical model for Raman scattering. The resonance Raman effect is then described by another model which emphasizes the discrete or quantized energy levels available to a molecule. The reader is also introduced to the experimental aspects of Raman spectroscopy and the application of Raman spectroscopy across the entire field of biochemistry. Each chapter contains an outline of the basic chemistry and biochemical nomenclature involved. This book will be of interest to chemists, biochemists, and spectroscopists, as well as graduate students and experienced research workers.

Table of Contents

  • Preface

    Chapter l. Introduction

    I. Raman Scattering

    II. The Appearance of a Raman Spectrum

    III. Basic Units

    IV. Raman, Resonance Raman, and Infrared Spectroscopies

    V. Advantages and Disadvantages of Raman Spectroscopy for Biochemical Studies

    Chapter 2. Principles of Raman Spectroscopy

    I. Introduction

    II. The Vibrations of a Diatomic Molecule from a Classical Standpoint

    III. A Classical Model for Raman Scattering

    IV. Comparison with Infrared Spectroscopy

    V. A Quantum Mechanical Picture of Raman Scattering

    VI. The Vibrations of a Polyatomic Molecule

    VII. Polarization Properties of Raman Scattering

    VIII. Raman Intensities

    Chapter 3. Experimental Raman Spectroscopy

    I. Basic Optics of the Raman Experiment

    II. Sampling Techniques and Sample Problems

    III. Sophisticated Techniques

    Chapter 4. Protein Conformation from Raman and Resonance Raman Spectra

    I. Protein Structure

    II. Introduction to Raman and Resonance Raman Studies of Proteins

    III. Raman Studies of Proteins

    IV. Resonance Raman Studies of the Chromophores of the Polypeptide Chain Using Excitation below 300 nm

    Chapter 5. Resonance Raman Studies of Natural, Protein-Bound Chromophores

    I. Introduction

    II. Heme Proteins

    III. Vitamin B12 (Cobalamin)

    IV. Chlorophylls

    V. Carotenoids

    VI. The Visual Pigments and Bacteriorhodopsin

    VII. Flavin Nucleotides

    VIII. Metalloproteins

    Chapter 6. Resonance Raman Labels

    I. Introduction

    II. Drug-Protein Complexes and Other Enzyme-Inhibitor Systems

    III. Antibody-Hapten Complexes

    IV. Permanently Labeled Sites—Arsanilazocarboxypeptidase A

    V. Enzyme-Substrate Reactions

    VI. Nucleic Acids and Membranes

    Chapter 7. Nucleic Acids and Nucleic Acid-Protein Complexes

    I. The Structure of Nucleic Acids

    II. Polynucleotide Structure from the Normal Raman Spectrum

    III. Applications to RNA and DNA Structures

    IV. Viruses and Other Nucleic Acid-Protein Complexes

    V. Resonance Raman Studies of Nucleic Acids

    Chapter 8. Lipids, Membranes, and Carbohydrates

    I. Structures of Lipids and Membranes

    II. Raman Spectra of Lipids and Membranes

    III. Carbohydrates

    Suggestions for Further Reading



Product details

  • No. of pages: 276
  • Language: English
  • Copyright: © Academic Press 1982
  • Published: July 28, 1982
  • Imprint: Academic Press
  • eBook ISBN: 9780323158121

About the Author

P Carey

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