Biocatalyst Immobilization

Biocatalyst Immobilization

Foundations and Applications

1st Edition - November 1, 2022

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  • Editor: Maria Ferreira
  • Paperback ISBN: 9780323913171

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Biocatalyst Immobilization: Foundations and Applications provides a comprehensive overview of biocatalytic immobilization processes, as well as methods for study, characterization, and application. Early chapters discuss current progress in enzyme immobilization and methods for selecting and pretreating enzymes prior to immobilization, with emphasis on navigating common challenges and employing enzyme supports and post immobilization treatments to impact enzymatic activity. Process-based chapters instruct in measuring and reporting on enzyme immobilization efficiency, protein final content, quantification of reaction products, and the use of nanomaterials to characterize immobilized enzymes. Later chapters examine recent advances, including novel enzymatic reactors, multi-enzymatic biocatalysts, enzymatic biosensors, whole cell immobilization, industrial application of immobilized enzymes, and perspectives on future trends.

Key Features

  • Provides a through overview of biocatalyst and enzyme immobilization for research and practical application
  • Methods based content instructs in enzyme immobilization pretreatment, enzyme supports, post immobilization treatments, measuring enzyme immobilization efficiency, quantification of reaction products, and whole cell immobilization
  • Features chapter contributions from international leaders in the field


Researchers in Biochemistry, Chemical Biology, Enzymatic Catalysis, Materials, Nanotechnology, and Applied Biotechnology; Students

Table of Contents

  • 1. Early supports and methods: the beginning and the key findings to progress
    Bern Nidetzky
    2. Selection and modification of enzymes prior to immobilization
    Debora de Oliveira
    3. Selection of supports for immobilization
    Alka Dwevedi
    4. Some challenges during immobilization
    José María Guisán
    5. Post -immobilization treatments before applications
    Roswanira Abdul Wahab
    6. Measuring and reporting immobilization efficiency
    Lassalle Nicolás and Ferreira Briand
    7. Various approaches to immobilization
    Zhongyu Yang
    8. Immobilization for use in non-conventional media
    Ferreira Foresti
    9. Assaying biological activities of the immobilized enzymes
    Ferreira Sánchez
    10. Support-free immobilization
    Sander van del Pelt
    11. Immobilization on nanomaterials and nano-structured supports
    Hafiz M.N. Iqbal and Ivo Safarik
    12. Hyphenated and non-hyphenated techniques in immobilized enzymes characterization
    Emilia Bramanti
    13. Some recent innovations related to enzyme immobilixation
    Pablo Dominguez de Maria
    14. Water-soluble supports, bioconjugates and smart immobilized enzymes
    H. Cheng and Norbert Sewald or Jiandong Cui
    15. Oriented Immobilization and Immobilization in small surfaces
    Shun'ichi Kuroda
    16. Affinity immobilization and affinity layers
    Michael A Nash
    17. New enzymatic reactor designs: from enzymatic batch to 3D microreactors and monoliths
    Jürgen Hubbuch
    18. Immobilization of Multi-Enzymes : Problems and Solutions
    Renchao Zheng
    19. An introduction to enzymatic Biosensors
    Hoang Hiep Nguyen
    20. Whole-Cell Immobilization at 2020
    Bo Liang
    21. Industrial Applications of immobilized enzymes: Food and beyond
    Elena F Krivoshapkina
    22. Final Future perspectives in enzyme immobilization
    Rafael Rodrigues

Product details

  • No. of pages: 432
  • Language: English
  • Copyright: © Academic Press 2022
  • Published: November 1, 2022
  • Imprint: Academic Press
  • Paperback ISBN: 9780323913171

About the Editor

Maria Ferreira

Dr. María Luján Ferreira is a Principles Investigator at CONICET (NATIONAL COUNCIL OF SCIENTIFIC AND TECHNICAL RESEARCH) in Buenos Aires, R. Argentina. Her research focuses on practical aspects of enzyme immobilization, including biocatalysis sampling in applied biotechnology. She has published 144 articles in peer reviewed, international journals, and has presented at over 80 conferences and forums worldwide. Dr. Ferreira has also directed three Assistant Investigators at CONICET.

Affiliations and Expertise

Principal Investigator, CONICET (National Council of Scientific and Technical Research), PLAPIQUI.-UNS-CONICET, Bahia Blanca, Buenos Aires, Argentina

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