Biocatalyst Immobilization

Biocatalyst Immobilization

Foundations and Applications

1st Edition - November 12, 2022

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  • Editor: Maria Ferreira
  • eBook ISBN: 9780323913775
  • Paperback ISBN: 9780323913171

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Biocatalyst Immobilization: Foundations and Applications provides a comprehensive overview of biocatalytic immobilization processes, as well as methods for study, characterization and application. Early chapters discuss current progress in enzyme immobilization and methods for selecting and pretreating enzymes prior to immobilization, with an emphasis on navigating common challenges and employing enzyme supports and post immobilization treatments to impact enzymatic activity. Process-based chapters instruct on measuring and reporting on enzyme immobilization efficiency, protein final content, quantification of reaction products, and the use of nanomaterials to characterize immobilized enzymes. Later chapters examine recent advances, including novel enzymatic reactors, multi-enzymatic biocatalysts, enzymatic biosensors, whole cell immobilization, the industrial application of immobilized enzymes, and perspectives on future trends.

Key Features

  • Provides a thorough overview of biocatalyst and enzyme immobilization for research and practical application
  • Presents methods based content that instructs in enzyme immobilization pretreatment, enzyme supports, post immobilization treatments, measuring enzyme immobilization efficiency, quantification of reaction products, and whole cell immobilization
  • Features chapter contributions from international leaders in the field


Researchers in Biochemistry, Chemical Biology, Enzymatic Catalysis, Materials, Nanotechnology, and Applied Biotechnology; Students

Table of Contents

  • CHAPTER 1 The enzyme, the support, and the immobilization strategy: The key findings to a desirable biocatalyst

    Karen Rodrıguez-Nunez, Claudia Bernal, and Juan M. Bolı´var

    CHAPTER 2 Selection and modification of enzymes prior to immobilization

    Ja Hyun Lee, Hah Young Yoo, and Seung Wook Kim

    CHAPTER 3 Various approaches to enzyme immobilization

    Syed Awais Attique, Qurat ul ain, Nazim Hussain, Muhammad Bilal, and Hafiz M.N. Iqbal

    CHAPTER 4 Postimmobilization treatments before applications

    Karolina Labus and Jolanta Bryjak

    CHAPTER 5 Support-free immobilization

    Roberta Bussons Rodrigues Val

    CHAPTER 6 Measuring and reporting enzyme´s immobilization efficiency

    Paula Nicolas, Carla Jos

    CHAPTER 7 Some recent innovations related to enzyme immobilization

    Bhaskar Das, Satyam, and Sanjukta Patra

    CHAPTER 8 Enzyme immobilization for use in nonconventional media

    Marıa Laura Foresti and Marıa Lujan Ferreira

    CHAPTER 9 Assaying the biological activities of immobilized enzymes

    Daniel Sanchez and Marıa Lujan Ferreira

    CHAPTER 10 Enzyme immobilization on nanomaterials and nanostructured supports

    Azeem Intisar, Mateen Haider, Muhammad Imran Din, Nazim Hussain, Muhammad Bilal, and Hafiz M.N. Iqbal

    CHAPTER 11 Oriented immobilization of biomolecules on small surfaces

    Masumi Iijima and Shunichi Kuroda

    CHAPTER 12 Affinity immobilization and affinity layers

    Meryam Sardar and Kainat Mirza

    CHAPTER 13 New enzymatic reactor designs: From enzymatic batch to 3D microreactors and monoliths

    Kim Shortall, Katarzyna Szymanska, Cristina Carucci, Tewfik Soulimane, and Edmond Magner

    CHAPTER 14 Immobilization of multienzymes: Problems and solutions

    Yingjie Du, Le Zhong, Ziyuan Wang, Yuxiao Feng, Jiandong Cui, and Shiru Jia

    CHAPTER 15 An introduction to enzymatic biosensors

    Tayyaba Anjum, Nazim Hussain, Muhammad Bilal, and Hafiz M.N. Iqbal

    CHAPTER 16 Industrial applications of immobilized enzymes: Food and beyond

    Marıa Lujan Ferreira

    CHAPTER 17 Future perspectives in enzyme immobilization

    Lorena Wilson, Andr

Product details

  • No. of pages: 458
  • Language: English
  • Copyright: © Academic Press 2022
  • Published: November 12, 2022
  • Imprint: Academic Press
  • eBook ISBN: 9780323913775
  • Paperback ISBN: 9780323913171

About the Editor

Maria Ferreira

PhD in Chemistry María Luján Ferreira is a Principal Investigator at CONICET (NATIONAL COUNCIL OF SCIENTIFIC AND TECHNICAL RESEARCH) of Argentina. She has published more than 150 articles in peer reviewed, international journals, and has participated with presentations in more than 80 argentinian scientific events and more than 120 international scientific meetings. Dr. Ferreira has also directed three Assistant Investigators at CONICET. Her research focuses on all practical aspects of enzyme immobilization (including applied biotechnology) and the details of the experimental design of enzymatic reactions: how to prepare immobilized biocatalysts and sampling in biocatalytic reactions ( from biphasic and multiphasic reaction media) avoiding mistakes and optimizing procedures. Her interests include the molecular modeling of biocatalysts. In more than 20 years of scientific career she has studied polymerization catalysts, heterogeneous metallic catalysts and recently also nanozymes and ionzymes applied in environmental remediation (of dyes and pharmaceuticals) using advanced oxidation processes.

Affiliations and Expertise

Principal Investigator, CONICET (National Council of Scientific and Technical Research), PLAPIQUI-UNS-CONICET, CCT Bahía Blanca, Bahía Blanca, Buenos Aires, Argentina.

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