Bioassay - 1st Edition - ISBN: 9781483230320, 9781483272764


1st Edition

Editors: Ralph I. Dorfman
eBook ISBN: 9781483272764
Imprint: Academic Press
Published Date: 1st January 1962
Page Count: 792
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Methods in Hormone Research, Volume II: Bioassay focuses on the detailed and critical account of bioassay methods, including electrolyte and carbohydrate methods, hormones, and assays. The selection first offers information on the statistical methods and steroid hormones and related substances. Topics include assays based on graded and quantal responses, measuring responses, types of estrogen, vaginal cornification, vaginal mitosis and epithelial thickness, and uterine weight. The book also ponders on anti-estrogenic compounds, progestational substances, and anti-gonadotropic steroids. The publication takes a look at androgens and anabolic agents, anti-androgenic substances, and corticoids. Discussions focus on surgical procedures, mammalian assays, bird, chick comb, electrolyte, and carbohydrate methods, adrenalectomy, and stress tests. The text then examines protein hormones and related hormones and substances, thyroidal substances, insulin, glucagon, and gonadotropins.
The selection is a valuable source of data for readers interested in bioassays.

Table of Contents


Contributors to Volume II


Contents, Volume I

Part I Statistical Methods

1. Statistical Methods

I. Introduction

II. Terminology and Procedure

III. Measuring Responses

IV. Assays Based On Graded Responses

V. Assays Based On Quantal Responses

VI. The Design of Assays


Part II Steroid Hormones and Related Substances

2. Estrogens

I. Introduction

II. Types of Estrogen

III. Vaginal Cornification

IV. Modifications of the Allen-Doisy Test

V. Interpreting Allen-Doisy Tests

VI. Intravaginal Allen-Doisy Tests

VII. Vaginal Mitosis and Epithelial Thickness

VIII. Vaginal Metabolic Activity

IX. Uterine Weight

X. Vaginal Opening

XI. In Vitro Assays

XII. Oral Dosage To Chicks

XIII. Duration of Action

XIV. Estrogen Inhibition


3. Anti-Estrogenic Compounds

I. Introduction

II. In Vivo Assays

III. In Vitro Assays

Iv. Conclusion


4. Progestational Substances

I. Introduction

II. Progestational Substances

III. Progestational Proliferation

IV. Endometrial Carbonic Anhydrase

V. Stromal Nuclear Hypertrophy

VI. Deciduoma Formation

VII. Pregnancy Maintenance and Parturition Delay

VIII. Anti-Fertility

IX. Chnical Evaluation of Progestational Agents

X. Concluding Remarks


5. Anti-Gonadotropic Steroids, Inhibition of Ovulation and Mating

I. Introduction

II. Parabiotic Technique

III. Development of the Parabiosis Method

IV. Inhibition of Gonadotropin In Intact Animals by Steroid Treatment

V. Reversal of Castration Changes In Pituitary Glands

VI. Inhibition of Luteinizing Hormone Activity of the Pituitary

VII. Inhibition of Ovulation

VIII. Inhibition of Fertility by Steroids


6. Androgens and Anabolic Agents

I. Introduction

II. Surgical Procedures

III. Bird Methods

IV. Mammahan Assays

V. Summary of Methods


7. Anti-Androgenic Substances

I. Introduction

II. Chick Comb Methods

III. Mammalian Methods

IV. Comments


8. Corticoids

I. Introduction

II. Adrenalectomy

Part III Protein Hormones and Related Hormones and Substances

9. Epinephrine and Norepinephrine

I. Introduction

II. Extraction and Separation

III. Bioassay


10. Thyroidal Substances

I. Introduction

II. Thyroidal Substances

III. Assays Based On Elevation of the Metabolic Rate

IV. Assays Based On Skeletal Changes

V. Assays Based On Goiter Prevention

VI. Assays Based On Use of Tadpoles

VII. Assays Based On Depression of Ii3i Uptake by Thyroid Gland

VIII. Methods of Determining Thyroxine Secretion Rate

IX. Thyroxine Secretion Rate


11. Insulin

I. Introduction

II. The Standard Preparation

III. The Rabbit Method of Assay

IV. The Mouse Method of Assay

V. Comparison of Rabbit and Mouse Methods of Assay

VI. Assay of Insulin At Physiological Levels

VII. Method Using Insulin Antiserum

VIII. Chromatographic Methods


12. Glucagon

I. Introduction

II. Methods of Assay

III. Measurement of Glucagon In Blood

IV. Summary


13. Parathyroid Hormone

I. Introduction

II. Possibilities of A Standard Preparation

III. The Unit of Parathyroid Activity

IV. Methods of Assay


14. Posterior Pituitary Hormones

I. Introduction

II. Standard Preparations

III. Methods of Assay


15. Melanophore-Expanding Activity

I. Introduction

II. Historical Resume

III. Theoretical Considerations

Iv. Methods Using The Whole Animal

V. Performance of An Assay

VI. Sample Assays

VII. Accuracy To Be Expected

VIII. Assay of Material Subjected To Heat at pH 12-13

IX. Methods Using Isolated Skin

X. Assay of Melanophore-Expanding Substance In Urine and Blood


16. Relaxin

I. Introduction

II. Standard Preparation

III. Guinea Pig Pubic Symphysis Methods of Assay

IV. Mouse Pubic Symphysis Methods of Assay

V. Assay of Relaxin by Inhibition of Uterine Motility

VI. Comparison of Relaxin Assay Methods

VII. Assay of Repository Forms of Relaxin

VIII. Other Actions of Relaxin Potentially Suitable For Bioassay Work

IX. Notes On The Stability of Relaxin


17. The Gonadotropins

I. Historical Introduction

II. Reference Standards

III. Use of Gonadotropin Assays

IV. Method of Urine Collection

V. Clinical Assay

VI. Bioassay


18. Prolactin

I. Historical Introduction

II. Assay Methods

III. Assay of Prolactin In Human Urine


19. Thyrotropic Hormone

I. Introduction

II. Factors Influencing The Sensitivity of the Thyroid Gland

III. Methods of Assay of Pituitary TSH

IV. Determination of TSH Secretion Rate

V. Bioassay of TSH In Blood Serum

Vi. Bioassay of TSH In Urine


20. Adrenocorticotropin

I. Introduction

II. Standard Preparations and Definition of Unit

III. In Vitro Assay Procedure (Saffran and Schally, 1955)

IV. Measurement of Corticosterone In Plasma and Adrenal Gland

V. Adrenal Ascorbic Acid As An End Point

VI. Other Assay Procedures

VII. Methods Other Than Ascorbic Acid

VIII. Choice of Procedure


21. Hypophyseal Growth Hormone

I. Introduction

II. Bioassay Based On Increment of Body Weight

III. The Tibia Test

IV. Immunological Tests

V. Miscellaneous Methods

VI. Concluding Remarks


Part IV Standard Methods

22. Standard Methods Adopted by Official Organizations

I. General Instructions For Hormone Assay Procedures

II. Specific Instructions For Hormone Assay Procedures

III. Interpretation of Biological Assay Data

Author Index

Subject Index


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© Academic Press 1962
Academic Press
eBook ISBN:

About the Editor

Ralph I. Dorfman

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