Bioaffinity Chromatography, Volume 55

2nd Edition

Authors: J. Turková
Hardcover ISBN: 9780444890306
eBook ISBN: 9780080858630
Imprint: Elsevier Science
Published Date: 14th September 1993
Page Count: 799
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Table of Contents

1. Introduction. 2. The principle, history and use of bioaffinity chromatography. 3. Choice of affinity ligands (affinants). 4. General considerations on affinant - sorbent bonding. 5. Solid matrix supports. 6. Survey of the most common coupling procedures. 7. Characterization of supports and immobilized affinity ligands. 8. General considerations on sorption, elution and non-specific binding. 9. Bioaffinity chromatography in the isolation, determination or removal of biologically active substances. 10. Immobilization of enzymes by biospecific adsorption to immobilized monoclonal or polyclonal antibodies. 11. Study of the modification, mechanism of action and structure of biologically active substances using bioaffinity chromatography. 12. Solid-phase immunoassay and enzyme-linked lectin assay. 13. Several examples of the application of biospecific adsorption in medicine. 14. Application of bioaffinity chromatography to the quantitative evaluation of specific complexes. 15. Theory of bioaffinity chromatography. Subject Index.

Description

Bioaffinity chromatography is now the preferred choice for the purification, determination or removal of many biologically active substances. The book includes information on biologically active substances with their affinants, solid supports and methods of coupling, summarized in tables covering classical, high-performance liquid and large-scale bioaffinity chromatography.

Optimization of the preparation and the use of highly active and stable biospecific adsorbents is discussed in several chapters. Following a chapter dealing with the choice of affinity ligands, affinity-sorbent bonding is described in detail. Other chapters give information on solid supports, the most common coupling procedures and a general discussion of sorption and elution. Several applications of bioaffinity chromatography are described, e.g. quantitative evaluation of biospecific complexes and many applications in medicine and in the biotechnology industry.


Details

No. of pages:
799
Language:
English
Copyright:
© Elsevier Science 1993
Published:
Imprint:
Elsevier Science
eBook ISBN:
9780080858630
Hardcover ISBN:
9780444890306

Reviews

@qu:Undoubtedly, this book is a major reference work on affinity chromatography that deserves a place in any biochemistry library. The author is to be congratulated for the enormous amount of work that must have gone into it. @source:The Analyst @qu:This is an excellent and very thorough overview of the field of bioaffinity and affinity chromatography... It is perhaps the paramount and ultimate reference book on bioaffinity chromatography, very modern, and very extensive. ...is highly recommended to any researcher just getting involved in one or more disciplines of affinity chromatography, or for anyone who is already experienced and expert. @source:Trends in Analytical Chemistry


About the Authors

J. Turková Author

Affiliations and Expertise

Czechoslovak Academy of Sciences, Institute of Organic Chemistry and Biochemistry, Prague, Czech Republic