Implementation of Mass Spectrometry Imaging in Pathology: Advances and Challenges
Key points
Mass spectrometry imaging
Mass spectrometry imaging applications in pathology
Challenges
Expectations and outlook
Clinics care points
Breath-Based Diagnosis of Infectious Diseases: A Review of the Current Landscape
Key points
Introduction
Breath matrices
Instruments for breath analysis
Diagnostic uses of clinical breath tests for infections
Summary
Clinics Care Points
Clinical Mass Spectrometry Approaches to Myeloma and Amyloidosis
Key points
Background
Use of mass spectrometry methods to detect plasma cell disorders
Use of mass spectrometry for the characterization of amyloid
Summary
Clinics care points
Breaking Through the Barrier: Regulatory Considerations Relevant to Ambient Mass Spectrometry at the Bedside
Key points
Ambient mass spectrometry in clinical applications; state of the art
Regulatory considerations in mass spectrometry–based assays: state of the art
Standardizing the workflow for validation of ambient mass spectrometry methods
Additional requirements associated with deployment within a clinical setting
Ambient mass spectrometry use cases in the clinical domain
Summary
Clinics care points
Glycan Imaging Mass Spectrometry: Progress in Developing Clinical Diagnostic Assays for Tissues, Biofluids, and Cells
Key points
Introduction
Method overview and applications of N-glycan matrix-assisted laser desorption/ionization imaging mass spectrometry of tissues
Limitations and method adaptations for N-glycan matrix-assisted laser desorption/ionization imaging mass spectrometry
Emerging uses of N-glycan matrix-assisted laser desorption/ionization imaging mass spectrometry for cancer tissue biomarkers and diagnostics
N-glycan imaging mass spectrometry of clinical biofluids
N-glycan imaging mass spectrometry of cultured cells
N-glycan imaging mass spectrometry of immunoarray-captured glycoproteins
Future directions
Summary
Matrix-Assisted Laser Desorption Ionization Time-of-Flight for Fungal Identification
Key points
Introduction
Matrix-assisted laser desorption ionization time-of-flight mass spectrometry for yeast identification
Matrix-assisted laser desorption ionization time-of-flight mass spectrometry for mold identification
Matrix-assisted laser desorption ionization time-of-flight mass spectrometry for fungal identification directly from positive blood culture bottles
Matrix-assisted laser desorption ionization time-of-flight mass spectrometry for antifungal resistance detection in yeasts and molds
Summary
Clinics care points
Mass Spectrometry and Microbial Diagnostics in the Clinical Laboratory
Key points
Introduction/history/definitions/background
Background
Discussion
Summary
Clinics care points
Mass Cytometry, Imaging Mass Cytometry, and Multiplexed Ion Beam Imaging Use in a Clinical Setting
Key points
Introduction
Mass cytometry
Imaging mass cytometry
Multiplexed ion beam imaging
Advantages of mass-tag detection
Advantages of high-parameter cellular characterization
Big data and high-dimensional approaches
Spanning-tree analysis of density normalized events
t-Distributed stochastic neighbor embedding/vi-distributed stochastic neighbor embedding and uniform manifold approximation and projection
Clinics care points
Summary
Bringing Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging to the Clinics
Key points
Introduction
Clinical workflows and opportunities
Clinical diagnostic applications
Summary and future directions
Funding
Clinics care points
No. of pages: 240
Language: English
Edition: 1
Volume: 41-2
Published: May 31, 2021
Imprint: Elsevier
Hardback ISBN: 9780323813297
eBook ISBN: 9780323813303
SB
Sankha (Bobby) Basu
Affiliations and expertise
Assistant Director of Clinical Chemistry and Mass Spectrometry
Associate Pathologist
Department of Pathology
Brigham and Women's Hospital Instructor of Pathology, Harvard Medical School