Basic Principles in Nucleic Acid Chemistry V2

Basic Principles in Nucleic Acid Chemistry V2

1st Edition - January 1, 1974

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  • Editor: Paul O.P. Ts'o
  • eBook ISBN: 9780323146890

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Basic Principles in Nuclear Acid Chemistry, Volume II presents the significant progress in nucleic acid research and its contribution and influence on various aspects of human life. This book contains five chapters and begins with the susceptibility of nucleic acids towards attack by chemical reagents whose reactions with polynucleotides have been studied. This topic is followed by a presentation of experimental techniques used to study the properties of nucleic acids. The following chapter discusses some basic features embodied in the polyribo- and poly-deoxyribonucleotide backbone chains; the possibility of rotation around backbone bonds in the ""random"" single-stranded form; and the short- and long-range interactions in idealized and real chains. This chapter also looks into the thermodynamic and polyelectrolyte aspects of nucleic acid behavior. A chapter describes the special features of the third class of DNA, namely, closed duplex DNA, in which covalent chain scissions are absent. The last chapter examines the intrinsic properties and the interaction of the dimers and oligomers, with special emphasis on the influence of the phosphodiester linkages on the conformation and interaction of these short segments of nucleic acids. This book is of great value to workers in biomedical research and to higher level biochemistry instructors.

Table of Contents

  • List of Contributors


    Contents of Other Volumes

    1. Chemical Reactions of Polynucleotides and Nucleic Acids

    I. Reactivity of Polynucleotides

    II. Base Modification by Nucleophilic Species

    III. Base Modification by Electrophilic Species

    IV. Reactions Affecting the Internucleotide Linkage

    V. Influence of Conformation on Reactivity in Polynucleotides


    2. Ultraviolet Spectroscopy, Circular Dichroism, and Optical Rotatory Dispersion

    I. Theoretical Introduction

    II. Nucleotide Chromophores

    III. Experimental Techniques—Ultraviolet Absorption Spectra

    IV. Experimental Techniques—Optical Activity


    3. Hydrodynamic and Thermodynamic Studies

    I. Introduction

    II. Basic Theoretical Aspects

    III. Specific Topics


    4. Circular DNA

    I. General Introduction

    II. The Topological Constraint

    III. The Special Thermodynamic Properties of Closed Double-Stranded DNA

    IV. The Tertiary Structure

    V. The Helix-Coil Transition

    VI. Reactions with Intercalating Substances

    VII. The Measurement of Superhelix Density

    VIII. Other Factors Which Affect the Superhelix Density

    IX. The Enzymic Preparation of Closed DNA of Preselected Superhelix Density

    X. Heterogeneity in Superhelix Density

    XL Complex Forms of Closed Circular DNA

    XII. Closed Circular Replicative Intermediates

    XIII. Circular DNA as a Substrate in Nucleic Acid Enzymology

    XIV. The Occurrence of Closed Circular DNA Glossary of Terms Used in the Description of Closed Circular DNA


    5. Dinucleoside Monophosphates, Dinucleotides, and Oligonucleotides

    I. Intrinsic Properties

    II. Interaction between Dinucleotides and Oligonucleotides

    III. Interaction between Oligonucleotides and Polynucleotides


    Author Index

    Subject Index

Product details

  • No. of pages: 532
  • Language: English
  • Copyright: © Academic Press 1974
  • Published: January 1, 1974
  • Imprint: Academic Press
  • eBook ISBN: 9780323146890

About the Editor

Paul O.P. Ts'o

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