Basic Life Science Methods

Basic Life Science Methods

A Laboratory Manual for Students and Researchers

1st Edition - October 14, 2022

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  • Authors: Javeed Tantray, Sheikh Mansoor, Rasy Wani, Nighat Un Nissa
  • Paperback ISBN: 9780443191749

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Description

Basic Life Science Methods: A Laboratory Manual for Students and Researchers is a perfect guide for graduate students and researchers in life sciences to execute and reproduce experiments. The book presents forty of the most executed life science assays. The authors use a consistent structure to cover the preparation, execution, and analysis of data from each method. Assays include estimation of cholesterol fractions, C-Reactive Protein, Genomic DNA isolation, Agarose Gel Electrophoresis, RT-PCR, DNA solution preparation, how to design primers, and enzyme-linked immunosorbent assay (ELISA). Basic Life Science Methods: A Laboratory Manual for Students and Researchers is a complete reference containing step-by-step instructions on how to run life science assays. Laboratory staff can also benefit of the book as a training resource.

Key Features

  • Provides a practical resource on designing, executing, and analyzing experiments and analytical procedures
  • Detailed and standardized coverage of basic research methods in the area
  • Provides step-by-step instructions on how to execute a large selection of life sciences experiments

Readership

Graduate students and early career researcher in life sciences. Life sciences laboratory staff members looking for resources to update their practices

Table of Contents

  • 1. Calculations of Molarity and Normality
    2. Glassware washing and sterilization techniques
    3. PH Meter: Its use and calibration
    4. Working and principles of Autoclave
    5. Working and principles of Centrifuge
    6. Working and principles of Laminar Airflow
    7. Estimation of Total cholesterol
    8. Estimation of Triglycerides
    9. Estimation of HDL – Cholesterol
    10. Estimation of LDL cholesterol
    11. Estimation of VLDL cholesterol
    12. Estimation of Creatinine
    13. Estimation of blood Urea
    14. Estimation of blood glucose By GOD – POD method
    15. C-Reactive Protein (CRP) slide agglutination test (Recombigen)
    16. Quantitative determinations of Homocysteine
    17. Estimation of serum bilirubin
    18. Estimation of diagnostic enzyme ALT
    19. Estimation of diagnostic enzyme AST
    20. To Estimate Protein by Lowry’s Method
    21. Estimation of Reducing sugar by using Dinitro Salicylic Acid (DNS) Method
    22. To perform quantitative determination of total protein in Serum/plasma
    23. Determination of Enzyme Activity Using Starch assay
    24. Estimation of Protein by Bradford’s Assay
    25. Identification of a Protein Sample Using SDS-PAGE
    26. Genomic DNA isolation
    27. PCR for gene amplification
    28. Agarose Gel Electrophoresis
    29. Polyacrylamide gel electrophoresis
    30. Real-time polymerase chain reaction (RT-PCR)
    31. Preparation of competent cells by CaCl2 treatment
    32. How to design primers
    33. DNA stock solution preparation
    34. Gel Electrophoresis
    35. DNA isolation from the bacterial culture
    36. DNA isolation from fungal Mycelium
    37. Quantification of DNA/RNA using spectrophotometer/Nanodrop
    38. Formaldehyde gel electrophoresis of RNA sample.
    39. ELISA (enzyme-linked immunosorbent assay)

Product details

  • No. of pages: 300
  • Language: English
  • Copyright: © Academic Press 2022
  • Published: October 14, 2022
  • Imprint: Academic Press
  • Paperback ISBN: 9780443191749

About the Authors

Javeed Tantray

Dr. Javeed Ahmad Tantray’s PhD current research focus is on Human cardiovascular diseases obtained his Ph. D from Osmania University in Hyderabad, India. Dr. Tantray has done Post Doc studies with the Department of Zoology, University of Kashmir, receiving funding from the UGC-CSIR. He published many National and International peer-reviewed research articles. Dr. Tantray has published in high impact factor journals such as the International Journal of Cardiology, and Saudi journal of biological science. He has guided 50, M.Sc / and graduate students projects. He has received Junior scientist award both at national and international level. He received Best Abstract Award at the 4th Asia Pacific CardioMetabolic syndrome Congress held by Korean Society of CardioMetabolic Syndrome,with award amount of USD 500. Dr. Tantray has organized a national seminar on "Cancer Bio-markers" and a one-day national workshop on “Assembly and usage of Foldscope”. Dr. Tantray is currently an Assistant Professor in the Department of Zoology, School of life sciences central university of Kashmir (J&K).

Affiliations and Expertise

Assistant Professor, Department of Zoology, Central University of Kashmir, India

Sheikh Mansoor

Dr. Sheikh Mansoor PhD is a postdoctoral researcher, presently working at the SK- Institute of Medical Sciences. He has obtained his BSc from SP College of Science (University of Kashmir), MSc from HNBGU Uttarakhand and a PhD degree from SKUAST Jammu. Dr. Mansoor has made important contributions to his field and has been working hard to shape himself as an impressive and contributively scientist of the highest intellectual calibre. He has an impressive lab experience in biochemistry, molecular biology and mice modelling to his credit. He has collaboratively worked and published research papers in highly reputed journals like frontiers, plus one, scientific reports, JoF MDPI etc. He has filed one patent application, which was published on 01/04/2022. Dr. Mansoor has delivered many guest lectures on sequence alignment, submission tools and phylogenetic analysis. He has submitted more than 150 sequences to NCBI GenBank. He has attended several national and international conferences, and workshops like European Molecular Biology Organisation (EMBO), and also received hands-on training on Advance Techniques in Modern Biology at Jamia Hamdard University. Dr. Sheikh Mansoor is review Editor in Frontiers in Ethnopharmacology, and also the reviewer of several reputed journals like 3 biotech, Cell Reports, Frontiers, Plosone, MDPI etc.

Affiliations and Expertise

Advance Centre for Human Genetics, Sheri Kashmir Institute of Medical Sciences, Soura, J&K - India

Rasy Wani

Rasy Fayaz Choh Wani PhD has completed her M.Sc. degree from Kashmir University. Ms. Rasy is currently pursuing Ph.D. She has published her research work in many reputed journals. She has presented her different papers in different international and national conferences. She is currently on research topic “Isolation and characterization of pathogenic bacterial microflora associated with cultured Cyprinus carpio in Kashmir Valley”.

Affiliations and Expertise

Research Scholar, Department of Zoology, University of Kashmir, India

Nighat Un Nissa

Nighat Un Nissa PhD has completed her M.Sc. and M.Phil. From Department of zoology, University of Kashmir. Ms. Nissa is currently pursuing her Ph.D. in Zoology and is working on Fish parasites and their impact on pollution. She has published her research work in National and International journals. She was awarded Gold Medal for poster presentation in Animal science congress held by Department of Zoology, University of Kashmir.

Affiliations and Expertise

Assistant Professor, Department of Zoology, University of Kashmir, India

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