Basic Life Science Methods
A Laboratory Manual for Students and Researchers
Free Global Shipping
No minimum orderDescription
Basic Life Science Methods: A Laboratory Manual for Students and Researchers is a perfect guide for graduate students and researchers in life sciences to execute and reproduce experiments. The book presents forty of the most executed life science assays. The authors use a consistent structure to cover the preparation, execution, and analysis of data from each method. Assays include estimation of cholesterol fractions, C-Reactive Protein, Genomic DNA isolation, Agarose Gel Electrophoresis, RT-PCR, DNA solution preparation, how to design primers, and enzyme-linked immunosorbent assay (ELISA). Basic Life Science Methods: A Laboratory Manual for Students and Researchers is a complete reference containing step-by-step instructions on how to run life science assays. Laboratory staff can also benefit of the book as a training resource.
Key Features
- Provides a practical resource on designing, executing, and analyzing experiments and analytical procedures
- Detailed and standardized coverage of basic research methods in the area
- Provides step-by-step instructions on how to execute a large selection of life sciences experiments
Readership
Graduate students and early career researcher in life sciences. Life sciences laboratory staff members looking for resources to update their practices
Table of Contents
- 1. Calculations of Molarity and Normality
2. Glassware washing and sterilization techniques
3. PH Meter: Its use and calibration
4. Working and principles of Autoclave
5. Working and principles of Centrifuge
6. Working and principles of Laminar Airflow
7. Estimation of Total cholesterol
8. Estimation of Triglycerides
9. Estimation of HDL – Cholesterol
10. Estimation of LDL cholesterol
11. Estimation of VLDL cholesterol
12. Estimation of Creatinine
13. Estimation of blood Urea
14. Estimation of blood glucose By GOD – POD method
15. C-Reactive Protein (CRP) slide agglutination test (Recombigen)
16. Quantitative determinations of Homocysteine
17. Estimation of serum bilirubin
18. Estimation of diagnostic enzyme ALT
19. Estimation of diagnostic enzyme AST
20. To Estimate Protein by Lowry’s Method
21. Estimation of Reducing sugar by using Dinitro Salicylic Acid (DNS) Method
22. To perform quantitative determination of total protein in Serum/plasma
23. Determination of Enzyme Activity Using Starch assay
24. Estimation of Protein by Bradford’s Assay
25. Identification of a Protein Sample Using SDS-PAGE
26. Genomic DNA isolation
27. PCR for gene amplification
28. Agarose Gel Electrophoresis
29. Polyacrylamide gel electrophoresis
30. Real-time polymerase chain reaction (RT-PCR)
31. Preparation of competent cells by CaCl2 treatment
32. How to design primers
33. DNA stock solution preparation
34. Gel Electrophoresis
35. DNA isolation from the bacterial culture
36. DNA isolation from fungal Mycelium
37. Quantification of DNA/RNA using spectrophotometer/Nanodrop
38. Formaldehyde gel electrophoresis of RNA sample.
39. ELISA (enzyme-linked immunosorbent assay)
Product details
- No. of pages: 300
- Language: English
- Copyright: © Academic Press 2022
- Published: October 14, 2022
- Imprint: Academic Press
- Paperback ISBN: 9780443191749
About the Authors
Javeed Tantray
Dr. Javeed Ahmad Tantray’s PhD current research focus is on Human cardiovascular diseases obtained his Ph. D from Osmania University in Hyderabad, India. Dr. Tantray has done Post Doc studies with the Department of Zoology, University of Kashmir, receiving funding from the UGC-CSIR. He published many National and International peer-reviewed research articles. Dr. Tantray has published in high impact factor journals such as the International Journal of Cardiology, and Saudi journal of biological science. He has guided 50, M.Sc / and graduate students projects. He has received Junior scientist award both at national and international level. He received Best Abstract Award at the 4th Asia Pacific CardioMetabolic syndrome Congress held by Korean Society of CardioMetabolic Syndrome,with award amount of USD 500. Dr. Tantray has organized a national seminar on "Cancer Bio-markers" and a one-day national workshop on “Assembly and usage of Foldscope”. Dr. Tantray is currently an Assistant Professor in the Department of Zoology, School of life sciences central university of Kashmir (J&K).
Affiliations and Expertise
Assistant Professor, Department of Zoology, Central University of Kashmir, India
Sheikh Mansoor
Dr. Sheikh Mansoor PhD is a postdoctoral researcher, presently working at the SK- Institute of Medical Sciences. He has obtained his BSc from SP College of Science (University of Kashmir), MSc from HNBGU Uttarakhand and a PhD degree from SKUAST Jammu. Dr. Mansoor has made important contributions to his field and has been working hard to shape himself as an impressive and contributively scientist of the highest intellectual calibre. He has an impressive lab experience in biochemistry, molecular biology and mice modelling to his credit. He has collaboratively worked and published research papers in highly reputed journals like frontiers, plus one, scientific reports, JoF MDPI etc. He has filed one patent application, which was published on 01/04/2022. Dr. Mansoor has delivered many guest lectures on sequence alignment, submission tools and phylogenetic analysis. He has submitted more than 150 sequences to NCBI GenBank. He has attended several national and international conferences, and workshops like European Molecular Biology Organisation (EMBO), and also received hands-on training on Advance Techniques in Modern Biology at Jamia Hamdard University. Dr. Sheikh Mansoor is review Editor in Frontiers in Ethnopharmacology, and also the reviewer of several reputed journals like 3 biotech, Cell Reports, Frontiers, Plosone, MDPI etc.
Affiliations and Expertise
Advance Centre for Human Genetics, Sheri Kashmir Institute of Medical Sciences, Soura, J&K - India
Rasy Wani
Rasy Fayaz Choh Wani PhD has completed her M.Sc. degree from Kashmir University. Ms. Rasy is currently pursuing Ph.D. She has published her research work in many reputed journals. She has presented her different papers in different international and national conferences. She is currently on research topic “Isolation and characterization of pathogenic bacterial microflora associated with cultured Cyprinus carpio in Kashmir Valley”.
Affiliations and Expertise
Research Scholar, Department of Zoology, University of Kashmir, India
Nighat Un Nissa
Nighat Un Nissa PhD has completed her M.Sc. and M.Phil. From Department of zoology, University of Kashmir. Ms. Nissa is currently pursuing her Ph.D. in Zoology and is working on Fish parasites and their impact on pollution. She has published her research work in National and International journals. She was awarded Gold Medal for poster presentation in Animal science congress held by Department of Zoology, University of Kashmir.
Affiliations and Expertise
Assistant Professor, Department of Zoology, University of Kashmir, India