Basic Biotechniques for Bioprocess and Bioentrepreneurship

Basic Biotechnique for Bioprocess and Bioentrepreneurship deals with the entire field of industrial biotechnology, starting from basic laboratory techniques, to scale-up, process development, demonstration and commercialization. The book compiles currently scattered materials on the topic and updates information based on practical experience and requirements.

• Offers guidance for readers on how to develop new microbiological and biotechnical processes
• Focuses on solutions to practical difficulties at all levels, creating a thorough understanding of bioprocess development, commercialization, technology transfer, and more
• Provides multifaceted coverage, with industry insights from leaders in the field
• Gives solutions to practical difficulties at all levels, i.e., Lab, Scale-Up and the Commercial stage

(post) graduate students and researchers in microbiology, biochemistry, biotechnology, environmental sciences and industrial professionals

Preface

UNIT-I : ISOLATION, SCREENING AND CULTURE MAINTENANCE

1. Isolation of microbes
M.K Gupta

2. Screening Strategies
Abhishek Walia, Chayanika Putatunda, Preeti Solanki, Sruthi Pathania and Anil Kumar

3. Identification: Morphological, Biochemical and genetic characterization
Nivedita Sharma and Sanjay Singh

4. Activity enhancing strategies: Enrichment and Statistical method
Shashi Kant Bhatia

5. Culture maintenance, preservation and improvement
Rakshak Kumar

UNIT-II : LABORATORY TECHNIQUES & INSTRUMENTATION

1. Biomolecules: Types, Homogenization, Bead beater, sonication
Arshad Jawed

2. Centrifugation: Basic principle, types
Gaurav Sood

3. Spectroscopy: Basic principle types and uses
Sweta Sinha and Ashutosh Upadhya

4. Protein Purification: Basic Principle and techniques
Deepak Pandey

5. Chromatography: Basic principle, types and applications
MAHESH K. GUPTA Sr. and Pradip Biswas

6. Electrophoresis: Basic principle, types and applications
Gopal Krishna Joshi

UNIT-III : GENOMIC AND PROTEOMIC ANALYSIS

1. DNA, RNA, isolation methods, primer designing, sequence submission, phylogenetic analysis
Neel Kamal Sharma

2. RDT and genetic engineering: basic of RDT method, PCR and application
Krishna Singh

3. Protein sequence analysis
Abhishek Chaudhary

4. Protein structure prediction
Vikash Gyeongsang

5. Docking strategies
Ragothaman M. Yennamalli

6. Methods of protein and drug interaction
Hannah Martin, Vidushi Agnihotri, Ragothaman M. Yennamalli and Aurijit Sarkar

UNIT-IV : INDUSTRIALLY IMPORTANT ENZYMES

1. Types of enzymes and their properties
Sheetal Verdhan

2. Enzyme kinetics: Industrially important enzymes
Kumar Sandeep

3. Industrial enzymes: Basic information, Assay and application
P.S. Panesar

UNIT-V : TECHNIQUES IN PROCESS DEVELOPMENT

1. Basic Fermentation
Umesh Narta and Nidhi Rana

2. Sterilization
Gunjan Goel

3. Laboratory and scale-up
Krunal Dholiya

4. Bioprocess: Control, management and Biosafety issues
Subhasish Duta

5. Demonstration and industrial scale up
Ramadoss Govindarajan

6. Downstream Processing
Dattatray Bedade

7. Waste Management & Environment
Narender Sharma and Nitin Endlay

UNIT-VI : FEASIBILITY, MARKETING AND BIOBUSINESS

1. Bio-business opportunities
Ashutosh Gupta and Gitika Nagrath

2. Feasibility and Business Plan
Narender Sharma

3. Patenting, IPR and Regulatory Issues
Sarita Devi Kapoor

4.Technology Transfer
Pradeep Kumar

5. Bio-product marketing strategies
Abhishek Goil