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Bailey & Scott's Diagnostic Microbiology
- 15th Edition - February 4, 2021
- Author: Patricia M. Tille
- Language: English
- Hardback ISBN:9 7 8 - 0 - 3 2 3 - 6 8 1 0 5 - 6
- eBook ISBN:9 7 8 - 0 - 3 2 3 - 6 8 1 0 6 - 3
Perfect your lab skills with the essential text for diagnostic microbiology! Bailey & Scott’s Diagnostic Microbiology, 15th Edition Is known as the #1 bench reference for pract… Read more
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Request a sales quotePerfect your lab skills with the essential text for diagnostic microbiology! Bailey & Scott’s Diagnostic Microbiology, 15th Edition Is known as the #1 bench reference for practicing microbiologists and as the preeminent text for students in clinical laboratory science programs. With hundreds of full-color illustrations and step-by-step methods for procedures, this text provides a solid, basic understanding of diagnostic microbiology and also covers more advanced techniques such as matrix-assisted laser desorption time-of-flight mass spectrometry. Written by noted CLS educator Dr. Patricia Tille, Diagnostic Microbiology has everything you need to get accurate lab test results in class and in clinical practice.
- Cover image
- Title page
- Disclaimer
- Table of Contents
- Copyright
- Dedication
- Reviewers
- Contributors
- Preface
- Part I. Basic Medical Microbiology
- 1. Microbial Taxonomy
- Classification
- Nomenclature
- Identification
- 2. Bacterial Genetics, Metabolism, and Structure
- Bacterial Genetics
- Bacterial Metabolism
- Structure and Function of the Bacterial Cell
- 3. Host-Microorganism Interactions
- The Encounter Between Host and Microorganism
- Microorganism Colonization of Host Surfaces
- Microorganism Entry, Invasion, and Dissemination
- Outcome and Prevention of Infectious Diseases
- General Principles in Clinical Microbiology
- Section 1. Safety and Specimen Management
- 4. Laboratory Safety
- Sterilization, Disinfection, and Decontamination
- Chemical Safety
- Fire Safety
- Electrical Safety
- Handling of Compressed Gases
- Biosafety
- Exposure Control Plan
- Employee Education and Orientation
- Disposal of Hazardous Waste
- Standard Precautions
- Laboratory Design and Engineering Controls
- Biological Safety Levels
- Mailing Biohazardous Materials
- 5. Specimen Management
- General Concepts for Specimen Collection and Handling
- Specimen Preservation
- Specimen Work-Up
- Expediting Results Reporting: Computerization
- Section 2. Approaches to Diagnosis of Infectious Diseases
- 6. Role of Microscopy
- Bright-Field (Light) Microscopy
- Phase-Contrast Microscopy
- Fluorescent Microscopy
- Dark-Field Microscopy
- Digital Automated Microscopy
- Digital Holographic Microscopy
- 7. Overview of Conventional Cultivation and Systems for Identification
- Organism Identification
- Principles of Bacterial Cultivation
- Bacterial Cultivation
- Principles of Identification
- Principles of Phenotypic Identification Schemes
- Commercial Identification Systems and Automation
- 8. Nucleic Acid–Based Analytic Methods for Microbial Identification and Characterization
- Overview of Nucleic Acid–Based Methods
- Postamplification End-Point Analysis
- 9. Overview of Immunochemical Methods Used for Organism Detection
- Features of the Immune Response
- Features of the Humoral Immune Response Useful in Diagnostic Testing
- Interpretation of Serologic Tests
- Production of Antibodies for Use in Laboratory Testing
- Immunoglobulin M Clinical Significance
- Separating Immunoglobulin M from Immunoglobulin G for Serologic Testing
- Principles of Immunochemical Methods Used for Organism Detection
- Summary
- Section 3. Evaluation of Antimicrobial Activity
- 10. Principles of Antimicrobial Action and Resistance
- Antimicrobial Action
- Mechanisms of Antibiotic Resistance
- 11. Laboratory Methods and Strategies for Antimicrobial Susceptibility Testing
- Goal and Limitations
- Testing Methods
- Laboratory Strategies for Antimicrobial Susceptibility Testing
- Accuracy
- Communication
- Bacteriology
- Section 1. Principles of Identification
- 12. Overview of Bacterial Identification Methods and Strategies
- Rationale for Approaching Organism Identification
- Future Trends of Organism Identification
- Section 2. Catalase-Positive, Gram-Positive Cocci
- 13. Staphylococcus, Micrococcus, and Similar Organisms
- General Characteristics
- Epidemiology
- Pathogenesis and Spectrum of Disease
- Laboratory Diagnosis
- Antimicrobial Susceptibility Testing and Therapy
- Prevention
- Section 3. Catalase-Negative, Gram-Positive Cocci
- 14. Streptococcus, Enterococcus, and Similar Organisms
- General Characteristics
- Epidemiology
- Pathogenesis and Spectrum of Disease
- Laboratory Diagnosis
- Antimicrobial Susceptibility Testing and Therapy
- Prevention
- Section 4. Non-Branching, Catalase-Positive, Gram-Positive Bacilli
- 15. Bacillus and Similar Organisms
- General Characteristics
- Laboratory Diagnosis
- Antimicrobial Susceptibility Testing and Therapy
- Prevention
- 16. Listeria, Corynebacterium, and Similar Organisms
- General Characteristics
- Epidemiology
- Pathogenesis and Spectrum of Disease
- Laboratory Diagnosis
- Antimicrobial Susceptibility Testing and Therapy
- Prevention
- Treatment
- Section 5. Non-Branching, Catalase-Negative, Gram-Positive Bacilli
- 17. Erysipelothrix, Lactobacillus, and Similar Organisms
- General Characteristics
- Epidemiology
- Pathogenesis and Spectrum of Disease
- Laboratory Diagnosis
- Prevention
- Section 6. Branching or Partially Acid-Fast, Gram-Positive Bacilli
- 18. Nocardia, Streptomyces, Rhodococcus, and Similar Organisms
- General Characteristics
- Epidemiology and Pathogenesis
- Laboratory Diagnosis
- Antimicrobial Susceptibility Testing and Therapy
- Prevention
- Section 7. Gram-Negative Bacilli and Coccobacilli (MacConkey-Positive, Oxidase-Negative)
- 19. Enterobacterales
- General Characteristics
- Epidemiology
- Pathogenesis and Spectrum of Diseases
- Specific Organisms
- Laboratory Diagnosis
- Prevention
- 20. Acinetobacter, Stenotrophomonas, and Other Organisms
- General Characteristics
- Epidemiology
- Pathogenesis and Spectrum of Disease
- Laboratory Diagnosis
- Cultivation
- Antimicrobial Resistance and Antimicrobial Susceptibility Testing
- Antimicrobial Therapy
- Prevention
- Section 8. Gram-Negative Bacilli and Coccobacilli (MacConkey-Positive, Oxidase-Positive)
- 21. Pseudomonas, Burkholderia, and Similar Organisms
- General Characteristics
- Epidemiology
- Pathogenesis and Spectrum of Disease
- Laboratory Diagnosis
- Antimicrobial Susceptibility Testing and Therapy
- Prevention
- 22. Achromobacter, Rhizobium, Ochrobactrum, and Similar Organisms
- General Characteristics
- Epidemiology
- Pathogenesis and Spectrum of Disease
- Laboratory Diagnosis
- Antimicrobial Susceptibility Testing and Therapy
- Prevention
- 23. Chryseobacterium, Sphingobacterium, and Similar Organisms
- General Characteristics
- Epidemiology
- Pathogenesis and Spectrum of Disease
- Laboratory Diagnosis
- Antimicrobial Susceptibility Testing and Therapy
- Prevention
- 24. Alcaligenes, Comamonas, and Similar Organisms
- General Characteristics
- Epidemiology
- Pathogenesis and Spectrum of Disease
- Laboratory Diagnosis
- Antimicrobial Susceptibility Testing and Therapy
- Prevention
- 25. Vibrio, Aeromonas, Plesiomonas shigelloides, and Chromobacterium violaceum
- General Characteristics
- Epidemiology
- Pathogenesis and Spectrum of Disease
- Laboratory Diagnosis
- Antimicrobial Susceptibility Testing and Therapy
- Prevention
- Section 9. Gram-Negative Bacilli and Coccobacilli (MacConkey-Negative, Oxidase-Positive)
- 26. Sphingomonas and Similar Organisms
- General Considerations
- Epidemiology, Spectrum of Disease, and Antimicrobial Therapy
- Laboratory Diagnosis
- Prevention
- 27. Moraxella and Neisseria spp.
- General Characteristics
- Epidemiology, Spectrum of Disease, and Antimicrobial Therapy
- Laboratory Diagnosis
- Antimicrobial Susceptibility
- Prevention
- 28. Eikenella corrodens and Similar Organisms
- General Characteristics
- Epidemiology, Spectrum of Disease, and Antimicrobial Therapy
- Laboratory Diagnosis
- Serodiagnosis
- Prevention
- 29. Pasteurella and Similar Organisms
- General Characteristics and Taxonomy
- Epidemiology, Spectrum of Disease, and Antimicrobial Therapy
- Laboratory Diagnosis
- Serodiagnosis
- Serodiagnosis
- Prevention
- 30. Actinobacillus, Kingella, Cardiobacterium, Capnocytophaga, and Similar Organisms
- General Characteristics
- Epidemiology, Pathogenesis, and Spectrum of Disease and Antimicrobial Therapy
- Laboratory Diagnosis
- Nucleic Acid Detection
- Serodiagnosis
- Prevention
- Section 10. Gram-Negative Bacilli and Coccobacilli (MacConkey-Negative, Oxidase-Variable)
- 31. Haemophilus
- General Characteristics
- Epidemiology
- Pathogenesis and Spectrum of Disease
- Laboratory Diagnosis
- Antimicrobial Susceptibility Testing and Therapy
- Prevention
- Section 11. Gram-Negative Bacilli That Are Optimally Recovered on Special Media
- 32. Bartonella
- Bartonella
- Direct Detection Methods
- 33. Campylobacter, Arcobacter, and Helicobacter
- Campylobacter and Arcobacter
- Helicobacter spp
- 34. Legionella
- General Characteristics
- Pathogenesis and Spectrum of Disease
- Laboratory Diagnosis
- Antimicrobial Susceptibility Testing and Therapy
- Prevention
- 35. Brucella
- General Characteristics
- Epidemiology and Pathogenesis
- Spectrum of Disease
- Laboratory Diagnosis
- Antimicrobial Susceptibility Testing and Therapy
- Prevention
- 36. Bordetella pertussis, Bordetella parapertussis, and Related Species
- General Characteristics
- Spectrum of Disease
- Laboratory Diagnosis
- Antimicrobial Susceptibility Testing and Therapy
- Prevention
- 37. Francisella
- General Characteristics
- Epidemiology and Pathogenesis
- Spectrum of Disease
- Laboratory Diagnosis
- Antimicrobial Susceptibility Testing and Therapy
- Prevention
- 38. Streptobacillus spp. and Spirillum minus
- Streptobacillus spp
- Spirillum minus
- Section 12. Gram-Negative Cocci
- 39. Neisseria and Moraxella catarrhalis
- General Characteristics
- Epidemiology
- Pathogenesis and Spectrum of Disease
- Laboratory Diagnosis
- Antimicrobial Susceptibility Testing and Therapy
- Prevention
- Section 13. Anaerobic Bacteriology
- 40. Overview and General Laboratory Considerations
- General Characteristics
- Specimen Collection and Transport
- Macroscopic Examination of Specimens
- Direct Detection Methods
- Specimen Processing
- Anaerobic Media
- Approach to Identification
- Antimicrobial Susceptibility Testing and Therapy
- 41. Overview of Anaerobic Organisms
- Epidemiology
- Pathogenesis and Spectrum of Disease
- Nucleic Acid Detection and MALDI-TOF MS (Gram-Positive)
- Nucleic Acid Detection and MALDI-TOF MS (Gram-Negative)
- Nucleic Acid Detection and MALDI-TOF MS
- Prevention
- Section 14. Mycobacteria and Other Bacteria With Unusual Growth Requirements
- 42. Mycobacteria
- Mycobacterium tuberculosis Complex
- Nontuberculous Mycobacteria
- Laboratory Diagnosis of Mycobacterial Infections
- Antimicrobial Susceptibility Testing and Therapy
- Prevention
- 43. Obligate Intracellular and Nonculturable Bacterial Agents
- Chlamydia
- Rickettsia, Orientia, Anaplasma, and Ehrlichia
- Coxiella
- Tropheryma whipplei
- Klebsiella granulomatis
- 44. Cell Wall–Deficient Bacteria: Mycoplasma and Ureaplasma
- General Characteristics
- Epidemiology and Pathogenesis
- Spectrum of Disease
- Laboratory Diagnosis
- Cultivation
- Susceptibility Testing and Therapy
- Prevention
- 45. The Spirochetes
- Treponema
- Borrelia
- Brachyspira
- Leptospira
- Prevention
- Part IV. Parasitology
- 46. Overview of the Methods and Strategies in Parasitology
- Epidemiology
- Pathogenesis and Spectrum of Disease
- Laboratory Diagnosis
- Approach to Identification
- Prevention
- Ectoparasites
- 47. Intestinal Protozoa
- Amoebae
- Flagellates
- Ciliates
- Sporozoa (Apicomplexa)
- Microsporidia
- 48. Blood and Tissue Protozoa
- Plasmodium spp
- Babesia spp
- Trypanosoma spp
- Leishmania spp
- 49. Protozoa From Other Body Sites
- Free-Living Amoebae
- Naegleria fowleri
- Acanthamoeba spp
- Acanthamoeba Keratitis
- Balamuthia mandrillaris
- Trichomonas vaginalis
- Toxoplasma gondii
- 50. Intestinal Nematodes
- Ascaris lumbricoides
- Enterobius vermicularis
- Strongyloides stercoralis
- Trichostrongylus spp
- Trichuris trichiura
- Capillaria philippinensis
- Hookworms
- Ancylostoma duodenale
- Necator americanus
- Ancylostoma ceylonicum, Ancylostoma braziliense, and Ancylostoma caninum
- Results and Reporting
- 51. Tissue Nematodes
- Trichinella spp
- Toxocara canis (Visceral Larva Migrans) and Toxocara cati (Ocular Larva Migrans)
- Baylisascaris procyonis (Neural Larva Migrans)
- Ancylostoma braziliense and Ancylostoma caninum (Cutaneous Larva Migrans)
- Dracunculus medinensis
- Parastrongylus cantonensis (Cerebral Angiostrongyliasis)
- Parastrongylus costaricensis (Abdominal Angiostrongyliasis)
- Gnathostoma spinigerum
- Capillaria hepatica
- Dirofilaria immitis and Other Species
- 52. Blood and Tissue Filarial Nematodes
- Wuchereria bancrofti
- Brugia malayi and Brugia timori
- Loa loa
- Onchocerca volvulus
- Mansonella spp. (M. ozzardi, M. streptocerca, M. perstans)
- Dirofilaria spp. (D. immitis, D. repens, D. tenuis)
- 53. Intestinal Cestodes
- Diphyllobothrium latum
- Dipylidium caninum
- Hymenolepis nana
- Hymenolepis diminuta
- Taenia solium
- Taenia saginata
- Taenia asiatica
- Taenia crassiceps
- 54. Tissue Cestodes
- Taenia solium
- Echinococcus granulosus Complex
- Echinococcus multilocularis
- Echinococcus oligarthrus and Echinococcus vogeli
- Taenia multiceps and Other Species
- Taenia serialis
- Spirometra mansonoides
- 55. Intestinal Trematodes
- Echinostoma spp
- Fasciolopsis buski General Characteristics
- Gastrodiscoides hominis
- Heterophyes: Metagonimus yokogawai, Centrocestus spp., Haplorchis spp., Stellantchamus spp., and Pygidiopsis spp. General Characteristics
- 56. Liver and Lung Trematodes
- The Liver Flukes
- The Lung Flukes
- 57. Blood Trematodes
- General Characteristics
- Epidemiology
- Pathology and Spectrum of Disease
- Laboratory Diagnosis
- Therapy
- Prevention
- Part V. Mycology
- 58. Overview of Fungal Identification Methods and Strategies
- Epidemiology
- General Features of the Fungi
- Taxonomy of the Fungi
- Clinical Classification of the Fungi
- Pathogenesis and Spectrum of Disease
- Laboratory Diagnosis
- General Considerations for the Identification of Yeasts
- General Considerations for the Identification of Molds
- General Morphologic Features of the Molds
- Clinical Relevance for Fungal Identification
- Laboratory Safety
- Prevention
- 59. Hyaline Molds, Mucorales, Basidiobolales, Entomophthorales, Dermatophytes, and Opportunistic and Systemic Mycoses
- The Mucorales
- The Entomophthorales and Basidiobolales
- The Dermatophytes
- The Opportunistic Mycoses
- Systemic Mycoses
- 60. Dematiaceous (Melanized) Molds
- General Characteristics
- Epidemiology and Pathogenesis Superficial Infections
- Pathogenesis and Spectrum of Disease
- Laboratory Diagnosis
- 61. Atypical and Parafungal Agents
- Pneumocystis
- Epidemiology
- Pathogenesis and Spectrum of Disease
- Laboratory Diagnosis
- Rare Atypical and Parafungal Agents
- 62. The Yeasts and Yeastlike Organisms
- General Characteristics
- Epidemiology
- Pathogenesis and Spectrum of Disease
- Laboratory Diagnosis
- Commercial Yeast Identification Systems
- 63. Antifungal Susceptibility Testing, Therapy, and Prevention
- Antifungal Susceptibility Testing
- Antifungal Therapy and Prevention
- Part VI. Virology
- 64. Overview of the Methods and Strategies in Virology
- General Characteristics
- Epidemiology
- Pathogenesis and Spectrum of Disease
- Prevention and Therapy
- Viruses That Cause Human Diseases
- Laboratory Diagnosis
- 65. Viruses in Human Disease
- Viruses in Human Disease
- Adenoviridae
- Arenaviridae
- Astroviridae
- Caliciviridae
- Coronaviridae
- Filoviridae
- Flaviviridae
- Hantaviridae
- Hepadnaviridae
- Hepeviridae
- Herpesviridae
- Herpesviruses
- Varicella-Zoster Virus
- Epstein-Barr Virus
- Cytomegalovirus
- Orthomyxoviridae
- Papillomaviruses
- Paramyxoviridae
- Measles Virus
- Parvoviridae
- Picornaviridae
- Enteroviruses, Parechoviruses, and Polioviruses
- Rhinovirus
- Hepatitis A Virus
- Pneumoviridae
- Polyomaviridae
- Poxviridae
- Reoviridae
- Retroviridae
- Rhabdoviridae
- Togaviridae
- Prions in Human Disease
- 66. Antiviral Therapy, Susceptibility Testing, and Prevention
- Antiviral Therapy
- Antiviral Resistance
- Methods of Antiviral Susceptibility Testing
- Prevention of Other Viral Infections
- Part VII. Diagnosis by Organ System
- 67. Bloodstream Infections
- General Considerations
- Detection of Bacteremia
- Special Considerations for Other Relevant Organisms Isolated From Blood
- 68. Infections of the Lower Respiratory Tract
- General Considerations
- Diseases of the Lower Respiratory Tract
- Laboratory Diagnosis of Lower Respiratory Tract Infections
- 69. Upper Respiratory Tract Infections and Other Infections of the Oral Cavity and Neck
- General Considerations
- Diseases of the Upper Respiratory Tract, Oral Cavity, and Neck
- Diagnosis of Upper Respiratory Tract Infections
- Diagnosis of Infections in the Oral Cavity and Neck
- 70. Meningitis and Other Infections of the Central Nervous System
- General Considerations
- Shunt Infections
- Laboratory Diagnosis of Central Nervous System Infections
- 71. Infections of the Eyes, Ears, and Sinuses
- Eyes
- Ears
- Sinuses
- 72. Infections of the Urinary Tract
- General Considerations
- Infections of the Urinary Tract
- Laboratory Diagnosis of Urinary Tract Infections
- 73. Genital Tract Infections
- General Considerations
- Genital Tract Infections
- Laboratory Diagnosis of Genital Tract Infections
- 74. Gastrointestinal Tract Infections
- Anatomy
- Resident Gastrointestinal Microbiome
- Gastroenteritis
- Other Infections of the Gastrointestinal Tract
- Laboratory Diagnosis of Gastrointestinal Tract Infections
- 75. Skin, Soft Tissue, and Wound Infections
- General Considerations
- Skin and Soft Tissue Infections
- Laboratory Diagnostic Procedures
- 76. Normally Sterile Body Fluids, Bone and Bone Marrow, and Solid Tissues
- Specimens From Sterile Body Sites
- Laboratory Diagnostic Procedures
- Part VIII. Clinical Laboratory Management
- 77. Quality in the Clinical Microbiology Laboratory
- Quality Program
- Specimen Collection and Transport
- Standard Operating Procedure Manual
- Personnel
- Reference Laboratories
- Patient Reports
- Proficiency Testing
- Performance Checks
- Antimicrobial Susceptibility Tests
- Maintenance of Quality Control Records
- Maintenance of Reference Quality Control Stocks
- Quality Assurance Program
- Types of Quality Assurance Audits
- Conducting a Quality Assurance Audit
- Continuous Daily Monitoring
- 78. Infection Control
- Incidence of Health Care–Associated Infections
- Types of Health Care–Associated Infections
- Emergence of Antibiotic-Resistant Microorganisms
- Hospital Infection Control Programs
- Role of the Microbiology Laboratory
- Characterizing Strains Involved in an Outbreak
- Preventing Health Care–Associated Infections
- Surveillance Methods
- 79. Sentinel Laboratory Response to Bioterrorism
- General Considerations
- Government Laws and Regulations
- Laboratory Response Network
- Index
- No. of pages: 1184
- Language: English
- Edition: 15
- Published: February 4, 2021
- Imprint: Elsevier
- Hardback ISBN: 9780323681056
- eBook ISBN: 9780323681063
PT
Patricia M. Tille
Affiliations and expertise
Chair of Microbiology Advisory Committee, Editor in Chief IJBLS, International Federation of Biomedical Laboratory Science, Graduate Program Director/Faculty, Medical Laboratory Science, University of Cincinnati