This volume of Methods in Enzymology contains contributions covering the wide spectrum of interactions between bacterial pathogens and their eukaryotic hosts. It complements Volume 235, in which methods for the isolation and identification of bacterial pathogens and associated virulence determinants are described. Since the study of bacterial pathogenesis borrows from a great variety of technologies and disciplines, we aim to provide the reader with a representative sample of the most advanced pathogenesis research.


Microbiologists, molecular biologists, biochemists, geneticists, pathologists, and immunologists (from MIE 235).

Table of Contents

Bacterial Effects on Immune Cells: H. Loppnow, H. Brade, E.T. Rietschel, and H.-D. Flad, Induction of Cytokines in Mononuclear and Vascular Cells by Endotoxin and Other Bacterial Products. M.S. Detrick, R. Kreisberg, and R.N. Moore, Effect of Bacterial Products on Colony-Stimulating Factor Production. Z.A. McGee and C.M. Clemens, Effect of Bacterial Products on Tumor Necrosis Factor Production: Quantitation in Biologic Fluids or Tissues. K.-I. Tanamoto, Induction of Prostaglandin Release from Macrophages by Bacterial Endotoxin. G. Sonnenfeld, Effect of Bacterial Products on Interferon Production. J.E. Puzas, D.G. Hicks, S.D. Reynolds, and R.J. OKeefe, Regulation of Osteoclastic Activityin Infection. J.R. Kalmar and T.E. Van Dyke, Effect of Bacterial Products on Neutrophil Chemotaxis. Phagocytosis and Killing Assays: R.F. Rest and D.P. Speert, Measurement of Nonopsonic Phagocytic Killing by Human and Mouse Phagocytes. J.R. Kalmar, Measurement of Opsonic Phagocytosis by Human Polymorphonuclear Neutrophils. R.F. Rest, Measurement of Human Neutrophil Respiratory Burst during Phagocytosis of Bacteria. D.P. McQuillen, S. Gulati, and P.A. Rice, Complement-Mediated Bacterial Killing Assays. T.H. Steinberg and J.A. Swanson, Measurement of Phagosome-Lyosome Fusion and Phagosomal pH. S.S.L. Harwig, T. Ganz, and R.I. Lehrer, Neutrophil Defensins: Purification, Characterization, and Antimicrobial Testing. J. Weiss, Purification and Assay of Bactericidal/Permeability-Increasing Protein. G. Storz and M.B. Toledano, Regulation of Bacterial Gene Expression in Response to Oxidative Stress. Ad


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