Bacterial Pathogenesis, Part A: Identification and Regulation of Virulence Factors, Volume 235

1st Edition

Print ISBN: 9780121821364
eBook ISBN: 9780080883489
Imprint: Academic Press
Published Date: 26th April 1994
Page Count: 789


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This volume of Methods in Enzymology presents methods for the isolation and identification of bacterial pathogens and associated virulence determinants. It will enable the researcher to determine if closed genes or isolated gene products are involved in virulence.

Key Features

@introbul:Major Topics Covered Include @bul:* Animal model system to determine bacterial virulence

  • Epidemiological techniques for the identification of bacterial strains and species
  • Protocols for the purification of subcellular bacterial products frequently associated with virulence
  • Determination of the means by which bacteria acquire iron
  • Methods to generate mutants, to construct isogenic strains by allelic exchange, to identify bacterial genes only expressed during infection of the host, and to study regulation of selected bacterial virulence factors
  • Methods for the assay of destructive bacterial enzymes and toxins


Microbiologists, molecular biologists, biochemists, geneticists, pathologists, and immunologists.

Table of Contents

S.A. Morse and J.E. McDade, Recommendations for Working with Pathogenic Bacteria.

Evaluations of Virulence in Animal Models:

S. Welkos and A. O'Brien, Determination of Median Lethal and Infectious Doses in Animal Model Systems.

D.J. Kopecko, Experimental Keratoconjunctivitis (Sereny) Assay.

R.D. Shahin and J.L. Cowell, Mouse Respiratory Infection Models for Pertussis.

B.A. Green, W.J. Doyle, and J.L. Cowell, Chinchilla Model of Experimental Otitis Media for Study of Nontypable Haemophilus influenzae Vaccine Efficacy.

R.G. Rank and J.A. Whittum-Hudson, Animal Models for Ocular Infections.

R.G. Rank, Animal Models for Urogenital Infections.

M.G. Tauber and A. Zwahlen, Animal Models for Meningitis.

T.E. Madden and J.G. Caton, Animal Models for Periodontal Disease.

C.A. Genco and R.J. Arko, Animal Chamber Models for Study of Host-Parasite Interactions.

D.F. Keren and M.A. Suckow, Animal Models for Immunoglobulin A Secretion.

Species and Strain Identification:

C.E. Frasch, Serogroup and Serotype Classification of Bacterial Pathogens.

K. Nelson and R.K. Selander, Analysis of Genetic Variation by Polymerase Chain Reaction-Based Nucleotide Sequencing.

R. Rudner, B. Studamire, and E.D. Jarvis, Determinations of Restriction Fragment Length Polymorphism in Bacteria Using Ribosomal RNA Genes.

D. van Soolingen, P.E.W. de Haas, P.W.M. Hermans, and J.D.A. van Embden, DNA Fingerprintingof Mycobacterium tuberculosis.

T.M. Schmidt and D.A. Relman, Phylogenetic Identification of Uncultured Pathogens Using Ribosomal RNA Sequences.

Bacterial Cell Fractionat


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© Academic Press 1994
Academic Press
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