This volume collates in one source methodology for in vivo genetic engineering and for genetic analysis in a wide range of bacteria. Not only is Escherichia coli well covered, but so are other emerging bacterial systems.


Biochemists, microbiologists, molecular biologists, geneticists, analytical chemists, biotechnologists, and graduate students in these disciplines.

Table of Contents

Escherichia coli and Salmonella typhimurium: J. Beckwith, Strategies for Finding Mutants. N.L. Sternberg and R. Maurer, Bacteriophage-Mediated Generalized Transduction in Escherichia coli and Salmonella typhimurium. K.B. Low, Conjugational Methods for Mapping with Hfr and F*b7 Strains D. Hanahan, J. Jessee, and F. Bloom, Plasmid Transformation of Escherichia coli and Other Bacteria. P.L. Foster, In Vivo Mutagenesis. T.A. Kunkel, K. Bebenek, and J. McClary, Efficient Site-Directed Mutagenesis Using Uracil-Containing DNA. N. Kleckner, J. Bender, and S. Gottesman, Uses of Transposons with Emphasis on Tn10. E.A. Groisman, In Vivo Genetic Engineering with Bacteriophage ~gm. J.M. Slauch and T.J. Silhavy, Genetic Fusions as~Experimental Tools. K.E. Sanderson and D.R. Zeigler, Storing, Shipping, and Maintaining Records on Bacterial Strains. M.L. Kahn, R. Ziermann, G. Deho, D.W. Ow, M.G. Sunshine, and R. Calendar, Bacteriophages P2 and P4. N.E. Murray, Special Uses of ~gl Phages for Molecular Cloning. Other Bacterial Systems: J.A. Hoch, Genetic Analysis in Bacillus subtilis. G.J. Barcak, M.S. Chandler, R.J. Redfield, and J.-F. Tomb, Genetic Systems in Haemophilus influenzae. M. So and H.S. Seifert, Genetic Systems in Pathogenic Neisseriae. D. Kaiser, Genetic Systems in Myxobacteria. B. Ely, Genetics of Caulobacter crescentus. G.A. Cangelosi, E.A. Best, G. Martinetti, and E.W. Nester, Genetic Analysis of Agrobacterium. J. Glazebrook and G.C. Walker, Genetic Techniques~in Rhiz


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© 1991
Academic Press
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