Bacillus Molecular Genetics and Biotechnology Applications - 1st Edition - ISBN: 9780122741555, 9780323138635

Bacillus Molecular Genetics and Biotechnology Applications

1st Edition

Authors: A.T. Ganesan
eBook ISBN: 9780323138635
Imprint: Academic Press
Published Date: 28th January 1986
Page Count: 497
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Bacillus Molecular Genetics and Biotechnology Applications contains the proceedings of the Third International Conference on the Genetics and Biotechnology of Bacilli, held at Stanford University in Stanford, California on July 15-17, 1985. Contributors discuss the progress that has been made concerning the molecular genetics and biotechnology of Bacillus and cover topics related to transposons and plasmids, secretion, gene cloning, and gene expression. This volume is organized into five sections encompassing 39 chapters and begins with an overview of the origin and the state of molecular genetics, along with some of the contributions microbiology has made to fundamental biology. It then emphasizes the importance of scientifically based regulatory decisions and responsible industry actions for effective biotechnology regulation. The chapters that follow focus on Bacillus subtilis, touching on topics such as DNA recombination in plasmids, genetic system for stabilizing cloned genes, regulation of sporulation, and non-complementing diploids. The reader is methodically introduced to the secretion and maturation of subtilisin, cloning in streptomycetes, and genetic exchange and prospects for cloning in Clostridia. The book concludes with a chapter that describes an integrative and amplifiable secretion vector using the inducible promoter and signal peptide from the B. subtilis levansucrase (LS) gene. This book will be of interest to geneticists, microbiologists, and biotechnologists, as well as students and researchers in the fields of molecular biology and biochemistry.

Table of Contents


Opening Session

Opening Address

The Roots and Shoots of Molecular Genetics

Keys to Effective Biotechnology Regulation: Scientific Based Decisions and Responsible Industry Action

Transposons and Plasmids

DNA Recombination in Plasmids and the Chromosome of Bacillus subtilis

A Genetic System for Stabilization of Cloned Genes in Bacillus subtilis

Membrane Binding and Regulation of Replication Initiation of pUB110 in Bacillus subtilis

Initiation of Chromosomal Replication in the Bacillus subtilis Mutant DNA 37

Further Characterization of the Inactive Chromosome from Bacillus subtilis Stabilized Noncomplementing Diploids


Construction of Secretion Vector and Secretion of hIFN-β

The Secretion and Maturation of Subtilisin

The Secreted Alkaline Phosphatase of Bacillus licheniformis MC14: Identification of a Possible Precursor

Production and Secretion of a Heterologous Protein by Bacillus subtilis Using Protease Gene-Based Secretion Vectors

Gene Cloning

Sequence Analysis and Function of Stage Ï Sporulation Genes

Genes Coding for Small, Acid Soluble Spore Proteins of Bacillus subtilis: Cloning, Expression, Regulation, Mapping, and Deletion

Regulation of Sporulation in Bacillus subtilis: The Structure and Function of spoOF

A Spore Germination Operon in Bacillus subtilis 168

The Bacillus subtilis DNA Polymerase III Gene Structure and Mode of Expression

Cloning and Sequencing of the Major Intracellular Serine Protease Gene of Bacillus subtilis

Gene Cloning and Expression Vectors Based on Bacillus subtilis Bacteriophage ϕ105

Homologous α-Amylases of Bacillus and Generation of Their Hybrids In Vivo

Cloning in Streptomycetes

Genetic Exchange and Prospects for Cloning in Clostridia

Cloning of Genes from Bacillus licheniformis Involved in Synthesis of the Peptide Antibiotic Bacitracin

The ilvBC-leu Operon of Bacillus subtilis

Complete Structure of an Insecticidal Crystal Protein Gene from Bacillus thuringiensis

Cloning of a Gene That Codes for a Mosquitocidal Toxin from Bacillus thuringiensis var. israelensis

Organization of Genes and Regulatory Sequences in the Origin Region of the Bacillus subtilis Chromosome: Remarkable Homology with Escherichia coli

Characterization of Cloned DNA from the Region between purA and attSPO2

Gene Expression

Organization of the Major Sigma Operons of Bacillus subtilis and Escherichia coli

Regulation of a Cloned Bacillus thuringienesis Crystal Protein Gene in Escherichia coli and Bacillus subtilis

Promoter Utilization by Sigma-29 RNA Polymerase

Characterization and Sequence of In Vivo ϕ29 Promoters by SI Mapping

Secretion Activity of the Bacillus subtilis α-Amylase Signal Peptides with Different Lengths in Bacillus subtilis and Escherichia coli Cells

Efficient Expression of Human Growth Hormone in Bacillus subtilis

Intergenic Utilization of Escherichia coli Transcriptional Signals

Cloning of Bacillus subtilis Promoters with the Help of the Alpha-Amylase Secretion Gene

Phenotypic Expression of CAT-86 in Bacillus subtilis Depends upon Chloramphenicol-Induced Translation of mRNA

Enhanced Gene Expression in Bacillus subtilis by Escherichia coli Insertion Sequences, 1S5 and IS1

Expression of a Thermostable Alpha-Amylase Gene under the Control of Levansucrase Inducible Promoter from Bacillus subtilis



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Academic Press
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A.T. Ganesan

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