B12 Enzymes

B12 Enzymes

1st Edition - June 1, 2022
This is the Latest Edition
  • Editor: Neil Marsh
  • Hardcover ISBN: 9780323915915

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Description

B12 Enzyme, Volume 667 in the Methods in Enzymology serial, highlights new advances in the field with this new volume presenting timely, interesting chapters, including Part I: B12 Biosynthesis and B12 Chaperones, Probing the preference and regioselectivity of lower ligand activation and its role in cobamide diversity, Biosynthesis of corrinoids, The human B12 trafficking chaperones: CblC, CblD and ATR, A method for the efficient adenosylation of corrinoids, Production, purification and liposome reconstitution of cobamide synthase, Extraction and Purification of Cobamides from Bacterial Cultures, Part II: The many faces of B12-dependent enzyme reactions, Methylcobalamin-dependent methionine synthase, and much more.

Key Features

  • Provides the authority and expertise of leading contributors from an international board of authors
  • Presents the latest release in Methods in Enzymology serials
  • Includes the latest information on B12 Enzymes

Readership

Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists

Table of Contents

  • B12 Biosynthesis and B12 Chaperones
    1. Probing the preference and regioselectivity of lower ligand activation and its role in cobamide diversity
    Amrita Hazra
    2. Biosynthesis of corrinoids
    Martin Warren
    3. The human B12 trafficking chaperones: CblC, CblD and ATR
    Ruma Banerjee
    4. A method for the efficient adenosylation of corrinoids
    Jorge C. Escalante-Semerena
    5. Production, purification and liposome reconstitution of cobamide synthase
    Jorge C. Escalante-Semerena
    6. Extraction and Purification of Cobamides from Bacterial Cultures
    Michiko E. Taga and Kenny Mok

    The many faces of B12-dependent enzyme reactions
    7. Methylcobalamin-dependent methionine synthase
    Markos Koutmos
    8. Heterologous production and biophysical characterization of reductive dehalogenases
    David Leys
    9. Photochemistry of coenzyme B12: methods for setting up radical solutions to transcriptional regulation
    Nigel Scrutton

    Adenosylcobalamin-dependent eliminases and isomerases
    10. Coenzyme B12-dependent dehydratases and a deaminase and their reactivating chaperones
    Tetsuo Toraya
    11. Analysis of radical-mediated catalysis by PLP- and adenosylcobalamin-dependent aminomutases using UV-visible and EPR spectroscopy
    Kirsten Wolthers
    12. Adenosylcobalamin-dependent carbon skeleton isomerases: glutamate mutase and 2- methyleneglutarate mutase
    Wolfgang Buckel
    13. Human B12 dependent enzymes: Methionine synthase and methylmalonyl-CoA mutase
    Ruma Banerjee

    Coenzyme B12-dependent radical SAM enzymes
    14. Structural characterization of cobalamin-dependent radical SAM methylases
    Squire Booker
    15. Purification and characterization of sequential cobalamin-dependent radical SAM methylases ThnK and TokK in carbapenem biosynthesis
    Craig Townsend and Erica Sinner
    16. Studies on the B12-dependent radical SAM methyltransferase Fom3
    Tadashi Eguchi
    17. Studies of OxsB and GenK, two B12-dependent radical SAM enzymes involved in natural product biosynthesis
    Hung-Wen Liu
    18. Purification and Structural Elucidation of a Cobalamin-dependent radical SAM enzyme
    Jennifer D. Rabb

    Methods for studying the mechanisms of B12 enzymes
    19. Synthesis and applications of "antivitamin" B12 derivatives
    Bernhard Kräutler
    20. Solvent-protein-reaction dynamical coupling in B12 enzyme catalysis
    Kurt Warncke
    21. Using kinetic isotope effects to probe the mechanisms of adenosylcobalamin-dependent enzymes
    E Neil G. Marsh
    22. Investigating radical pair reaction dynamics of B12 coenzymes using magnetic field effects
    Alex Jones
    23. Computational investigations of B12-dependent enzymatic reactions
    Pawel Kozlowski

    Spectroscopic methods applied to B12 enzymes
    24. Time-resolved spectroscopy: Advances in understanding the electronic structure and dynamics of cobalamins
    Roseanne Sension and James Penner-Hahn
    25. Investigating radical pair reaction dynamics of B12 coenzymes using time-resolved electron paramagnetic resonance spectroscopy
    Alex Jones
    26. MCD and Raman Spectroscopy of cobalamin-dependent enzymes
    Thomas C. Brunold
    27. Structural characterization of radical intermediates in reaction of lysine 5,6- aminomutase by EPR, ENDOR, and ESEEM
    Shyue-Chu Ke
    28. Kazunari Yoshizawa

Product details

  • No. of pages: 412
  • Language: English
  • Copyright: © Academic Press 2022
  • Published: June 1, 2022
  • Imprint: Academic Press
  • Hardcover ISBN: 9780323915915
  • About the Serial Volume Editor

    Neil Marsh

    I am currently Professor of Chemistry and Biological Chemistry at the University of Michigan in Ann Arbor. My research interests center on enzyme mechanisms and protein structure and design. We are currently working on a variety of research projects. In particular, we have a long-standing interest in enzymes that use free radicals to catalyze a variety of unusual chemical transformations. More recently, we have become interested in enzymes involved in hydrocarbon biosynthesis, many of which have novel mechanisms and are of practical interest for the biosynthesis of next-generation biofuels. We are also interested in understanding in molecular detail how enzymes interact with abiological surfaces as this is key to many industrial and biomedical applications where enzymes are immobilized on solid supports. More information about my research can be found here:http://www.lsa.umich.edu/chem/people/faculty/ci.marsheneilg_ci.detail

    Affiliations and Expertise

    Dow Distinguished Faculty Fellow in Sustainability, Professor of Chemistry and Biological Chemistry, Department of Chemistry, University of Michigan, USA