Avidin-Biotin Technology, Volume 184

1st Edition

Serial Volume Editors: Meir Wilchek Edward Bayer
Hardcover ISBN: 9780121820855
eBook ISBN: 9780080883014
Imprint: Academic Press
Published Date: 28th May 1990
Page Count: 746

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Table of Contents

Introduction: F.M. Richards, Reflections. M. Wilchek and E.A. Bayer, Introduction to Avidin-Biotin Technology. M. Wilchek and E.A. Bayer, Applications of Avidin-Biotin Technology: Literature Survey. Biotin-Binding Proteins: E.A. Bayer and M. Wilchek, Biotin-Binding Proteins: Overview and Prospects. N.M. Green, Avidin and Streptavidin. Y. Hiller, E.A. Bayer, and M. Wilchek, Nonglycosylated Avidin. G. Chandra and J.G. Gray, Cloning and Expression of Avidin in Escherichia coli. E.A. Bayer, H. Ben-Hur, and M. Wilchek, Isolation and Properties of Streptavidin. O. Livnah and J.L. Sussman, Crystal Forms of Avidin. K. Dakshinamurti and J. Chauhan, Non-Avidin Biotin-Binding Proteins. B. Wolf, J. Hymes, and G.S. Heard, Biotinidase. K. Dakshinamurti and E.S. Rector, Monoclonal Antibody to Biotin. General Methodology: Preparation of Biotin Derivatives: M. Wilchek and E.A. Bayer, Biotin-Containing Reagents. E.A. Bayer and M. Wilchek, Protein Biotinylation. A. Schwarz, C. Wandrey, E.A. Bayer, and M. Wilchek, Enzymatic C-Terminal Biotinylation of Proteins. D.J. O'Shannessy, Antibodies Biotinylated via Sugar Residues. B.F. Goldin and G.A. Orr, 2-Iminobiotin-Containing Reagent and Affinity Columns. Preparation of Avidin and Streptavidin Derivatives: E.A. Bayer and M. Wilchek, Avidin- and Streptavidin-Containing Probes. A.N. Glazer and L. Stryer, Phycobiliprotein-Avidin and Phycobiliprotein-Biotin Conjugates. R.A. Kohanski and M.D. Lane,

Description

The avidin-biotin complex has been used for isolation (affinity chromatography), localization (affinity cytochemistry, cell cytometry, and blotting technology), and diagnostics (immunoassay, histopathology, and gene probes). Recently, usage of the system has been extended to include other areas. This volume covers these new applications and methodologies including hybridoma technology, bioaffinity sensors, affinity targeting, and drug delivery, as well as cross-linking, immobilization, and fusogenic studies.

Readership

Biochemists, molecular biologists, immunologists, microbiologists, endocrinologists, biotechnologically oriented industries, analytical chemists, and clinical chemists.


Details

No. of pages:
746
Language:
English
Copyright:
© Academic Press 1990
Published:
Imprint:
Academic Press
eBook ISBN:
9780080883014
Hardcover ISBN:
9780121820855

Reviews

@from:Praise for the Volume @qu:"This text would be useful for specialists in the areas of biophysics, biochemistry, immunology, molecular biology, etc., and others who are interested in the recent advances in avidin-biotin research." @source:--BIOLOGICAL ABSTRACTS @from:Praise for the Series @qu:"The Methods in Enzymology series represents the gold-standard." @source:--NEUROSCIENCE @qu:"Incomparably useful." @source:--ANALYTICAL BIOCHEMISTRY @qu:"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." @source:--BIO/TECHNOLOGY @qu:"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." @source:--CHEMISTRY IN INDUSTRY @qu:"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." @source:--AMERICAN SOCIETY OF MICROBIOLOGY NEWS @qu:"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work." @source:--ENZYMOLOGIA @qu:"A series that has established itself as a definitive reference for biochemists." @source:--JOURNAL OF CHROMATOGRAPHY


About the Serial Volume Editors

Meir Wilchek Serial Volume Editor

Affiliations and Expertise

The Weizmann Institute of Science, Rehovot, Israel

Edward Bayer Serial Volume Editor

Affiliations and Expertise

The Weizmann Institute of Science, Rehovot, Israel