Avidin-Biotin Technology, Volume 184

1st Edition

Serial Volume Editors: Meir Wilchek Edward Bayer
Editor-in-Chiefs: John Abelson Melvin Simon
Hardcover ISBN: 9780121820855
eBook ISBN: 9780080883014
Imprint: Academic Press
Published Date: 28th May 1990
Page Count: 746


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Table of Contents


F.M. Richards, Reflections.

M. Wilchek and E.A. Bayer, Introduction to Avidin-Biotin Technology.

M. Wilchek and E.A. Bayer, Applications of Avidin-Biotin Technology: Literature Survey.

Biotin-Binding Proteins:

E.A. Bayer and M. Wilchek, Biotin-Binding Proteins: Overview and Prospects.

N.M. Green, Avidin and Streptavidin.

Y. Hiller, E.A. Bayer, and M. Wilchek, Nonglycosylated Avidin.

G. Chandra and J.G. Gray, Cloning and Expression of Avidin in Escherichia coli.

E.A. Bayer, H. Ben-Hur, and M. Wilchek, Isolation and Properties of Streptavidin.

O. Livnah and J.L. Sussman, Crystal Forms of Avidin.

K. Dakshinamurti and J. Chauhan, Non-Avidin Biotin-Binding Proteins.

B. Wolf, J. Hymes, and G.S. Heard, Biotinidase.

K. Dakshinamurti and E.S. Rector, Monoclonal Antibody to Biotin.

General Methodology: Preparation of Biotin Derivatives:

M. Wilchek and E.A. Bayer, Biotin-Containing Reagents.

E.A. Bayer and M. Wilchek, Protein Biotinylation.

A. Schwarz, C. Wandrey, E.A. Bayer, and M. Wilchek, Enzymatic C-Terminal Biotinylation of Proteins.

D.J. O'Shannessy, Antibodies Biotinylated via Sugar Residues.

B.F. Goldin and G.A. Orr, 2-Iminobiotin-Containing Reagent and Affinity Columns.

Preparation of Avidin and Streptavidin Derivatives:

E.A. Bayer and M. Wilchek, Avidin- and Streptavidin-Containing Probes.

A.N. Glazer and L. Stryer, Phycobiliprotein-Avidin and Phycobiliprotein-Biotin Conjugates.

R.A. Kohanski and M.D. Lane,


The avidin-biotin complex has been used for isolation (affinity chromatography), localization (affinity cytochemistry, cell cytometry, and blotting technology), and diagnostics (immunoassay, histopathology, and gene probes). Recently, usage of the system has been extended to include other areas. This volume covers these new applications and methodologies including hybridoma technology, bioaffinity sensors, affinity targeting, and drug delivery, as well as cross-linking, immobilization, and fusogenic studies.


Biochemists, molecular biologists, immunologists, microbiologists, endocrinologists, biotechnologically oriented industries, analytical chemists, and clinical chemists.


No. of pages:
© Academic Press 1990
Academic Press
eBook ISBN:
Hardcover ISBN:


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About the Serial Volume Editors

Meir Wilchek Serial Volume Editor

Affiliations and Expertise

The Weizmann Institute of Science, Rehovot, Israel

Edward Bayer Serial Volume Editor

Affiliations and Expertise

The Weizmann Institute of Science, Rehovot, Israel

About the Editor-in-Chiefs

John Abelson Editor-in-Chief

Affiliations and Expertise

California Institute of Technology, Division of Biology, Pasadena, U.S.A.

Melvin Simon Editor-in-Chief

Affiliations and Expertise

The Salk Institute, La Jolla, CA, USA