Autoradiography in Biology and Medicine

Autoradiography in Biology and Medicine

1st Edition - January 1, 1955

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  • Author: George A. Boyd
  • eBook ISBN: 9781483282336

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Description

Autoradiography in Biology and Medicine focuses on the applications of autoradiography in medicine and biology, including photographic processes, emulsions, and response of films to isotopes and x-rays. The book first offers information on the meaning of autoradiography, including the etymology of autoradiography, photographic emulsion as a scientific instrument, and the relationship of biologists and autoradiography. The publication then examines the photographic process and comparative response of commercial and scientific emulsions. Discussions focus on fixation, washing, emulsion, response of films to electrons, x-rays, and gamma rays, and response of films to beta ray spectra of isotopes. The text takes a look at commercial photographic materials for autoradiography, autoradiographic image, and resolution. The manuscript then reviews the estimation of dose and exposure time, sources of error, and techniques. Topics include chemical variables, estimation of exposure time, errors occurring during the preparation of the autoradiogram for exposure, and contour autoradiography. The book is a valuable reference for readers interested in autoradiography.

Table of Contents


  • Contents

    Part I Theory

    I. Introduction

    Historical, What Is Autoradiography?, The Photographic Emulsion As A Scientific Instrument, The Biologist and Autoradiography, The Etymology of Autoradiography

    II. The Photographic Process

    Introduction, The Emulsion, The Latent Image, Development, Latent-Image Potential, Fixation, Washing, Drying

    III. Comparative Response of Commercial and Scientific Emulsions

    Introduction, Response of Films To Electrons, Response of Films To Beta Ray Spectra of Isotopes, Response of Films to X-Rays and Gamma Rays

    IV. Commercial Photographic Materials For Autoradiography

    X-Ray Films, Nuclear Emulsions, Stripping Films and Plates, Gels and Pellicles, X-Ray Emulsions on Plate

    V. The Autoradiographic Image

    Observing The Image, Track Images, Random Grain Images, Realignment Techniques, Publishing The Autoradiogram

    VI. Resolution

    Theoretical Considerations, Experimental Results on Variables Affecting Resolution

    VII. Estimating Dose and Exposure Time

    Type of Information Desired, Chemical Variables, Variables of The Biological Laboratory, Autoradiographic Technique, Doses Reported in The Literature, Estimating The Exposure Time

    VIII. Sources of Error

    Leaching The Radioactivity During the Histologic Preparation, Errors Occurring During the Preparation of The Autoradiogram for Exposure, Errors Occurring During Exposure—Chemical Effects, Errors Occurring During Exposure—Other Miscellaneous, Errors Occurring During Photographic and Histologic Processing, Other Errors

    Part II Techniques

    Introduction

    IX. Nonflat Surfaces

    Contour Autoradiography, Autoradiography Plus Roentgenography of Patients

    X. Flat Surfaces—Macrosections and Plants

    Freezing and Slicing Soft Tissues, Bones Embedded in Plaster of Paris, Bones Mounted on Boards, Freezing and Sawing Soft and Hard Tissues Combined, Plants

    XI. Flat Surfaces—Microsections

    Apposition Methods for Microscopic Sections and Smears, Wet Mounting of Tissue Sections, Dry Mounting of Tissue Sections, Coating With Liquid Emulsion—Zoological Materials, Coating With Liquid Emulsion—Plant Materials, Coating With Permeable-Base Stripping Film, Coating and Inverting, Impermeable-Base Stripping Film Technique, The Wet Process, Constant Registry Autoradiography

    XII. Cells, Dusts, and Other Microscopic Particulate Matter

    Impermeable-Base Stripping Film Technique, Smearing Suspensions of Cells on A Photographic Plate, Staining Bone-Marrow Cells Through Permeable-Base Stripping Film, Air-Borne and Other Particular Matter

    XIII. Fluids

    XIV. Techniques for Preparing Histologic Sections of Undecalcified Bone and Teeth

    Embedding in Bio-Plastic, Embedding in M4700 With Plasticizer, Embedding in M4700 without Plasticizer, Rapid Embedding in R-250 Astrolite, Rapid Embedding in Methyl Methacrylate, Microsawing of Histologic Sections

    XV. Miscellaneous

    Thin Protective Films, Transferring Frozen Histologic Sections to Plates and Slides, Probe Autoradiography, Autoradiography by Photoelectron Emission Microscopy, Pinhole Autoradiography

    Part III Bibliography

    1. Introduction

    2. Bibliography

    3. Author Index to the Bibliography

    4. Classified Subject Index to the Bibliography

    5. Supplement to the Bibliography

    6. Books for Supplemental Reading

    Index


Product details

  • No. of pages: 414
  • Language: English
  • Copyright: © Academic Press 1955
  • Published: January 1, 1955
  • Imprint: Academic Press
  • eBook ISBN: 9781483282336

About the Author

George A. Boyd

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