Autoradiography for Biologists - 1st Edition - ISBN: 9780122732508, 9780323161053

Autoradiography for Biologists

1st Edition

Editors: P Gahan
eBook ISBN: 9780323161053
Imprint: Academic Press
Published Date: 1st January 1972
Page Count: 136
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Autoradiography for Biologists discusses the basic and practical aspects of various autoradiography techniques. It is composed of six chapters; each chapter covers a specific form of autoradiography technique. Chapter 1 discusses various theoretical aspects of autoradiography, such as radioactive decay, beta-particle absorption, photographic process, sensitivity, and resolving power. Chapters 2 to 6 discuss basic aspects of several forms of autoradiography, including macro-, stripping film, diffusible substances, liquid emulsion, and high resolution autoradiography. Practical and theoretical details of the technique, together with modifications made, are covered in each chapter. This book will be both an encouragement and a help to people wanting to apply autoradiography to the solution of biological problems.

Table of Contents

Contributors Preface Chapter 1. Theory of Autoradiography I. Introduction II . Radioactive Decay and Absorption of ß-particles III . Photographic Process IV. Sensitivity V. Resolving Power VI. Techniques VII. Difficulties and Artefacts VIII. Quantative Autoradiography References Chapter 2. Macro-Autoradiography I. Introduction II . Darkroom Requirements III . Amounts of Isotopes IV. Choice of Emulsion V. Tissue Preparation and Application VI. Exposure Times VII. Processing of the Autoradiography VIII. Artefacts References Chapter 3. Stripping Film Autoradiography I. Introduction II . Solutions Required III . Preparation of Histological Material IV. Setting up Autoradiographs
V. Autoradiographic Exposure VI. Development and Fixation VII. Staining VIII. Mounting References Chapter 4. Autoradiography of Diffusible Substances I. Introduction II . Solutions Required III . Freezing or Quenching the Tissues IV. Storage of Frozen Materials V. Filmed Coverslips VI. Sectioning VII. "Picking-up" VIII. Exposure IX. Processing X. Staining XI. Mounting References Chapter 5. Liquid Emulsion Autoradiography I. Introduction II . Preparation of Tissues III . Darkroom Requirements IV. Choice of Emulsion V. Dipping Technique for Light Microscopy Autoradiography VI. Processing of Autoradiographs VII. Staining after Processing of Autoradiographs (poststaining) VIII. Mounting of Preparations IX. Special Techniques X. General Comments XI. Summary of Procedure for Coating Tissue Sections with Liquid Emulsion References Chapter 6. High Resolution Autoradiography I. Introduction II . Available Methods of High Resolution Autoradiography I


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© Academic Press 1972
Academic Press
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About the Editor

P Gahan

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