Autoradiography for Biologists

Autoradiography for Biologists

1st Edition - January 1, 1972

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  • Editor: P Gahan
  • eBook ISBN: 9780323161053

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Description

Autoradiography for Biologists discusses the basic and practical aspects of various autoradiography techniques. It is composed of six chapters; each chapter covers a specific form of autoradiography technique. Chapter 1 discusses various theoretical aspects of autoradiography, such as radioactive decay, beta-particle absorption, photographic process, sensitivity, and resolving power. Chapters 2 to 6 discuss basic aspects of several forms of autoradiography, including macro-, stripping film, diffusible substances, liquid emulsion, and high resolution autoradiography. Practical and theoretical details of the technique, together with modifications made, are covered in each chapter. This book will be both an encouragement and a help to people wanting to apply autoradiography to the solution of biological problems.

Table of Contents


  • Contributors

    Preface

    Chapter 1. Theory of Autoradiography

    I. Introduction

    II . Radioactive Decay and Absorption of ß-particles

    III . Photographic Process

    IV. Sensitivity

    V. Resolving Power

    VI. Techniques

    VII. Difficulties and Artefacts

    VIII. Quantative Autoradiography

    References

    Chapter 2. Macro-Autoradiography

    I. Introduction

    II . Darkroom Requirements

    III . Amounts of Isotopes

    IV. Choice of Emulsion

    V. Tissue Preparation and Application

    VI. Exposure Times

    VII. Processing of the Autoradiography

    VIII. Artefacts

    References

    Chapter 3. Stripping Film Autoradiography

    I. Introduction

    II . Solutions Required

    III . Preparation of Histological Material

    IV. Setting up Autoradiographs

    V. Autoradiographic Exposure

    VI. Development and Fixation

    VII. Staining

    VIII. Mounting

    References

    Chapter 4. Autoradiography of Diffusible Substances

    I. Introduction

    II . Solutions Required

    III . Freezing or Quenching the Tissues

    IV. Storage of Frozen Materials

    V. Filmed Coverslips

    VI. Sectioning

    VII. "Picking-up"

    VIII. Exposure

    IX. Processing

    X. Staining

    XI. Mounting

    References

    Chapter 5. Liquid Emulsion Autoradiography

    I. Introduction

    II . Preparation of Tissues

    III . Darkroom Requirements

    IV. Choice of Emulsion

    V. Dipping Technique for Light Microscopy Autoradiography

    VI. Processing of Autoradiographs

    VII. Staining after Processing of Autoradiographs (poststaining)

    VIII. Mounting of Preparations

    IX. Special Techniques

    X. General Comments

    XI. Summary of Procedure for Coating Tissue Sections with Liquid Emulsion

    References

    Chapter 6. High Resolution Autoradiography

    I. Introduction

    II . Available Methods of High Resolution Autoradiography

    III . Requirements for Quantitative Electron Microscope Autoradiography

    IV. The preparation of High Resolution Autoradiographs

    V. Increasing Contrast in Electron Microscope Autoradiographs

    VI. The Measurement of Efficiency

    VII. Resolution in High Resolution Autoradiography

    VIII. Applications of High Resolution Autoradiography

    Acknowledgements

    References

    Subject Index


Product details

  • No. of pages: 136
  • Language: English
  • Copyright: © Academic Press 1972
  • Published: January 1, 1972
  • Imprint: Academic Press
  • eBook ISBN: 9780323161053

About the Editor

P Gahan

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