The third and final installment of Daniel J. Klionsky's new three-volume treatment of autophagy, this volume focuses on monitoring autophagy with regard to disease connections, and presents methods that can be used to analyze autophagy in clinical samples. Edited by one of the leading authorities in the field, this volume and its companion volumes, Autophagy: Lower Eukaryotes and Autophagy in Mammalian Systems, provide a comprehensive overview of the techniques involved in studying autophagy in eukaryotes and simple animal systems, mammalian cells and non-human animals, and humans. Particularly in times of stress, like starvation and disease, higher organisms have an internal mechanism in their cells for chewing up and recycling parts of themselves. The process of internal “house cleaning” in the cell is called autophagy – literally self-eating. In the future, research in this field will help to design clinical approaches that can turn on autophagy and halt tumor growth.

Key Features

* Provides an overview of autophagy in regards to humans, specifically regarding disease connections and clinical samples. * Includes methods to evaluate the role of autophagy in the drug-induced cell death of cancer cells in culture *Presents reliable methods that, in this relatively new field, allow the reader to find appropriate techniques to identify, monitor, and quantify autophagic processes


Researchers and students in biochemistry, cell and molecular biology, oncology, pharmacology

Table of Contents

1. Initiation of Autophagy by Photodynamic Therapy Nancy L. Oleinick and David Kessel 2. Autophagic cell death Michael Lenardo and Li Yu 3. Autophagic neuron death Yasuo Uchiyama, Masato Koike, Masahiro Shibata, and Mizuho Sasaki 4. Assessing Metabolic Stress and Autophagy Status in Epithelial Tumors Robin Mathew, Vassiliki Karantza-Wadsworth and Eileen White 5. Autophagic clearance of aggregate–prone proteins associated with neurodegeneration Sovan Sarkar, Brinda Ravikumar and David Rubinsztein 6. Monitoring Autophagy in Alzheimer's Disease and Related Neurodegenerative Diseases Sovan Dun-Sheng Yang, Ju-Hyun Lee; Ralph A. Nixon 7. Live-cell imaging of autophagy induction and autophagosome-lysosome fusion in primary cultured neurons Mona Bains and Kim A. Heidenreich 8. Using Genetic Mouse Models to Study the Biology and Pathology of Autophagy in the Central Nervous System Zhenyu Yue*, Gay R. Holstein, Brian T. Chait and Qing Jun Wang 9. Biochemical and Morphological Detection of Inclusion Bodies in Autophagy-deficient Mice Masaaki Komatsu and Satoshi Waguri 10. Analyzing Autophagy in Clinical Tissues of Lung and Vascular Diseases Augustine Choi, Zhi Hua Chen, Hong Pyo Kim and Stefan Ryter 11. Autophagy in Neurite Injury and Neurodegeneration: in vitro and in vivo models Charleen T. Chu, Edward D. Plowey, Robert W. Hickey, Ruben K. Dagda and Robert S.B. Clark 12. MONITORING THE AUTOPHAGY PATHWAY IN CANCER John L. Cleveland, Frank Dorsey and Meredith Steeves 13. Autophagy pathways i


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© 2009
Academic Press
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About the editor

Daniel Klionsky

Affiliations and Expertise

Life Sciences Institute, University of Michigan, USA