Description

A timely book for DNA researchers, Automated DNA Sequencing and Analysis reviews and assesses the state of the art of automated DNA sequence analysis-from the construction of clone libraries to the developmentof laboratory and community databases. It presents the methodologies and strategies of automated DNA sequence analysis in a way that allows them to be compared and contrasted. By taking a broad view of the process of automated sequence analysis, the present volume bridges the gap between the protocols supplied with instrument and reaction kits and the finalized data presented in the research literature. It will be an invaluable aid to both small laboratories that are interested in taking maximum advantageof automated sequence resources and to groups pursuing large-scale cDNA and genomic sequencing projects.

Key Features

* The field of automation in DAN sequencing and analysis is rapidly moving. Hovever, as the technology becomes commonplace, those applying the techniques involved to their research fields need a text which both expands on the protocols supplied by manufacturers with their instruments and explains how to utilise the data produced. This book fulfils those needs, reviews the history of the art and provides pointers to future development.

Readership

Reference text for researchers and technicians using automated DNA sequencers for studies in molecular genetics, genome sciences, and cell biology.

Table of Contents

Sequencing Instruments and Strategies: E. Chen, The Efficiency of Automated DNA Sequencing. G.M. Church, G. Gryan, N. Lakey, S. Kieffer-Higgins, L. Mintz, M. Temple, M. Rubenfield, L. Jaehn, H. Ghazizadel, K. Robison, and P. Richterick, Automated Multiplex Sequencing. X.C. Huang and R.A. Mathies, Application of Capillary Array Electrophoresis to DNA Sequencing. R. Drmanac, S. Drmanac, J. Jarvis, and I. Labat, Sequencing by Hybridization. A. Martin-Gallardo, J. Lamerdin, and A. Carrano, Shotgun Sequencing. A. Bodenteich, S. Chissoe, Y.-F. Wang, and B. Roe, Shotgun Cloning as the Strategy of Choice to Generate Templates for High Throughput Dideoxynucleotide Sequencing. C.M. Berg, G. Wang, K. Isono, H. Kasai, and D.E. Berg, Transposon-Facilitated Large-Scale DNA Sequencing. C.H. Martin, C.A. Mayeda, C.A. Davis, M.P. Strathmann, and M. Palazzolo, Transposon-Facilitated Sequencing: An Effective Set of Procedures to Sequence DNA Fragments Smaller than 4kb. L. Liu and R.D. Fleischmann, Construction of Exonuclease III Nested Deletion Sets for Rapid DNA Sequencing. M. Adams, Expressed Sequence Tags as Tools for Physiology and Genomics. W.R. McCombie, The Use of Automated DNA Sequencing in the Analysis of cDNAs of Model Organisms. Sample Preparation and Sequencing Methods: Libraries: R.L. Stalligs, N.A. Doggett, A. Ford, J. Longmire, C.E. Hildebrand, L.L. Deaven, and R. Moyzis, Applications of Cosmid Libraries in Genome Mapping and Sequencing Efforts. D.A. Smoller, W.J. Kimmerly, O. Hubbard, C. Ericsson, C.H. Martin, and M.J. Palazzolo, A Role for the P1 Cloning System in Genome Analysis. N.A. Doggett, D.L. Grady, J.L. Longmire, and

Details

No. of pages:
368
Language:
English
Copyright:
© 1994
Published:
Imprint:
Academic Press
Print ISBN:
9780127170107
Electronic ISBN:
9780080926391