Assessment of Immune status by the Leukocyte Adherence Inhibition Test - 1st Edition - ISBN: 9780126897500, 9780323152297

Assessment of Immune status by the Leukocyte Adherence Inhibition Test

1st Edition

Editors: D Thomson
eBook ISBN: 9780323152297
Imprint: Academic Press
Published Date: 1st January 1982
Page Count: 398
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Assessment of Immune Status by the Leukocyte Adherence Inhibition Test reviews many aspects of the immune response and cellular requirements in leukocyte adherence inhibition (LAI). It compares the LAI methodology with alternative approaches, discusses the method that has been adopted to determine whether the LAI phenomenon measures a specific immune response, and explores how these phenomena fit in with and relate to the traditional immune response. Organized into three parts encompassing 15 chapters, this volume begins with an overview of the hemocytometer assay, the immunological basis and applications of hemocytometer LAI, the immune response as assayed by the test tube method, and the microplate assay. It then discusses cellular populations in LAI; measurements of humoral antitumor immunity; clinical application of LAI in breast cancer, prostate cancer, and rheumatic diseases; the biology of LAI and its response to human cancer; and cellular and pharmacologic aspects of LAI. It also compares microplate LAI with other in vitro assays of cell-mediated immunity, as well as tumor immunity in animal models and human cancer. Both beginning and experienced investigators involved in all aspects of immunologic research will find this book extremely useful.

Table of Contents

List of Contributors


I Leukocyte Adherence Inhibition on Hemocytometers

1 Hemocytometer LAI: Immunological Basis and Applications

I. Introduction

II. Historical Survey

III. Technical Summary

IV. Reaction Mechanism

V. Immunodiagnosis of Human Cancer

VI. Some Comparisons and Relationships

VII. Future Prospects


2 Cellular Populations in Leukocyte Adherence Inhibition

I. Introduction

II. LAI Methods

III. Cellular Interactions

IV. Quantitative Aspects

V. Conclusions


3 Measurements of Humoral Antitumor Immunity

I. Introduction

II. H-LAI Measurements

III. Nature of Humoral Factor

IV. Mechanisms of the H-LAI Response

V. H-LAI Measurements in Cancer Detection

VI. Conclusions


4 Clinical Application in Breast Cancer

I. Introduction

II. Antigen Preparation

III. Specificity of LAI Assay

IV. Mechanism of LAI

V. LAI Assay among Family Members of Breast Cancer Patients

VI. LAI and Serum Blocking Factors

VII. Arming Phenomenon with Breast Cancer Patients' Sera

VIII. Evolution of LAI at the City of Hope

IX. Conclusion


II Leukocyte Adherence Inhibition in Test Tubes

5 The Use of the Assay to Measure Immunity in Animal Models

I. Introduction

II. Methodological Aspects

III. Specificity, Mechanism, and Biological Significance

IV. Discussion


6 The LAI Response to Human Cancer: The Biology of the LAI Response and Features of the Antigens

I. Introduction

II. Computerized Tube LAI Assay

III. Oncofetal Organ-Specific Neoantigens of Human Cancer

IV. Biology of the LAI Response

V. Modulation of the Expression of Human Antitumor Immunity

VI. Diagnostic Value

VII. Summary


7 Leukocyte Adherence Inhibition, Carcinoembryonic Antigen, and Tumor Size in Patients with Colorectal Carcinoma

I. Introduction

II. Methods and Technical Problems

III. Results


8 Evaluation of Leukocyte Adherence Inhibition in Prostate Cancer

I. Introduction

II. Hemocytometer LAI


IV. Disease-Specific Reactivity

V. Tumor-Specific Reactivity

VI. Effect of Therapy

VII. Evaluation of LAI Data

VIII. Conclusion


9 The Leukocyte Adherence Inhibition Assay in Human and Experimental Rheumatic Diseases

I. Arthritic Disorders Evaluated Utilizing LAI Assay

II. Standardization of the Tube LAI Assay

III. Results of LAI Assay in Rheumatoid Arthritis

IV. Results of LAI Assay in Experimental Arthritis

V. Significance of LAI Studies in Experimental Arthritis

VI. Significance of LAI Studies in Rheumatoid Arthritis

VII. Conclusions


10 Cellular and Pharmacologic Aspects

I. Introduction

II. The Test in Arthritis

III. Significance of the Nonadherence in the LAI

IV. Cellular Aspects

V. Sensitization of IgG Rheumatoid Factor

VI. LAI Test and Pregnancy Serum

VII. Characterization of Synovial Extract

VIII. Activation of Polymorphonuclear Leukocytes

IX. Leukocyte Adherence and Prostaglandins

X. Leukocyte Adherence and ß Receptors

XI. LAI Test with Synovial Fluid Leukocytes

XII. Adherence and Immunomodulation

XIII. Discussion


III Leukocyte Adherence Inhibition in the Microplate

11 Comparison of Microplate LAI with Other in Vitro Assays of Cell-Mediated Immunity: Assessment of Responses to Bacterial, Viral, and Tumor Antigens in Man and Experimental Animals

I. Microplate LAI

II. Microplate LAI Reactivity in Murine Tumor Systems

III. Microplate LAI in the Assessment of Host Responses to Viral and Bacterial Antigens

IV. Conclusions


12 A Comparison of Tumor Immunity in Animal Models and Human Cancer

I. Introduction

II. Method

III. Characterization of Antigen(s) Detected in the Microplate LAI Assay

IV. Patterns of Sensitization Detected by the Microplate LAI Assay

V. Serum Studies

VI. Mechanism of Reactivity

VII. Summary


13 Cellular Requirements for the Leukocyte Adherence Inhibition Response to an Immunogenic Viral Induced Murine Tumor

I. Introduction

II. Methodology

III. LAI Response of Mice to Regressing MSV-lnduced Tumors

IV. LAI Response to Progressively Growing Tumors

V. Mechanisms of the LAI Response to MSV-lnduced Tumors

VI. Comparison with Mouse MIF Formation

VII. Conclusions


14 Leukocyte Adherence Inhibition Testing in Domestic Animals

I. Introduction

II. Methods Available for the Evaluation of Cellular Immunity in Domestic Animals

III. LAI Testing

IV. Experimental Use of the LAI Microassay

V. Conclusions


15 Leukocyte Adherence Inhibition: Measurement by Radioisotopic Labeled Cells

I. Introduction

II. Assay Using 111Indium-Labeled Cells




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© Academic Press 1982
Academic Press
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D Thomson

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