Assessment of Immune status by the Leukocyte Adherence Inhibition Test

Assessment of Immune status by the Leukocyte Adherence Inhibition Test

1st Edition - January 1, 1982

Write a review

  • Editor: D Thomson
  • eBook ISBN: 9780323152297

Purchase options

Purchase options
DRM-free (PDF)
Sales tax will be calculated at check-out

Institutional Subscription

Free Global Shipping
No minimum order


Assessment of Immune Status by the Leukocyte Adherence Inhibition Test reviews many aspects of the immune response and cellular requirements in leukocyte adherence inhibition (LAI). It compares the LAI methodology with alternative approaches, discusses the method that has been adopted to determine whether the LAI phenomenon measures a specific immune response, and explores how these phenomena fit in with and relate to the traditional immune response. Organized into three parts encompassing 15 chapters, this volume begins with an overview of the hemocytometer assay, the immunological basis and applications of hemocytometer LAI, the immune response as assayed by the test tube method, and the microplate assay. It then discusses cellular populations in LAI; measurements of humoral antitumor immunity; clinical application of LAI in breast cancer, prostate cancer, and rheumatic diseases; the biology of LAI and its response to human cancer; and cellular and pharmacologic aspects of LAI. It also compares microplate LAI with other in vitro assays of cell-mediated immunity, as well as tumor immunity in animal models and human cancer. Both beginning and experienced investigators involved in all aspects of immunologic research will find this book extremely useful.

Table of Contents

  • List of Contributors


    I Leukocyte Adherence Inhibition on Hemocytometers

    1 Hemocytometer LAI: Immunological Basis and Applications

    I. Introduction

    II. Historical Survey

    III. Technical Summary

    IV. Reaction Mechanism

    V. Immunodiagnosis of Human Cancer

    VI. Some Comparisons and Relationships

    VII. Future Prospects


    2 Cellular Populations in Leukocyte Adherence Inhibition

    I. Introduction

    II. LAI Methods

    III. Cellular Interactions

    IV. Quantitative Aspects

    V. Conclusions


    3 Measurements of Humoral Antitumor Immunity

    I. Introduction

    II. H-LAI Measurements

    III. Nature of Humoral Factor

    IV. Mechanisms of the H-LAI Response

    V. H-LAI Measurements in Cancer Detection

    VI. Conclusions


    4 Clinical Application in Breast Cancer

    I. Introduction

    II. Antigen Preparation

    III. Specificity of LAI Assay

    IV. Mechanism of LAI

    V. LAI Assay among Family Members of Breast Cancer Patients

    VI. LAI and Serum Blocking Factors

    VII. Arming Phenomenon with Breast Cancer Patients' Sera

    VIII. Evolution of LAI at the City of Hope

    IX. Conclusion


    II Leukocyte Adherence Inhibition in Test Tubes

    5 The Use of the Assay to Measure Immunity in Animal Models

    I. Introduction

    II. Methodological Aspects

    III. Specificity, Mechanism, and Biological Significance

    IV. Discussion


    6 The LAI Response to Human Cancer: The Biology of the LAI Response and Features of the Antigens

    I. Introduction

    II. Computerized Tube LAI Assay

    III. Oncofetal Organ-Specific Neoantigens of Human Cancer

    IV. Biology of the LAI Response

    V. Modulation of the Expression of Human Antitumor Immunity

    VI. Diagnostic Value

    VII. Summary


    7 Leukocyte Adherence Inhibition, Carcinoembryonic Antigen, and Tumor Size in Patients with Colorectal Carcinoma

    I. Introduction

    II. Methods and Technical Problems

    III. Results


    8 Evaluation of Leukocyte Adherence Inhibition in Prostate Cancer

    I. Introduction

    II. Hemocytometer LAI

    III. Tube LAI

    IV. Disease-Specific Reactivity

    V. Tumor-Specific Reactivity

    VI. Effect of Therapy

    VII. Evaluation of LAI Data

    VIII. Conclusion


    9 The Leukocyte Adherence Inhibition Assay in Human and Experimental Rheumatic Diseases

    I. Arthritic Disorders Evaluated Utilizing LAI Assay

    II. Standardization of the Tube LAI Assay

    III. Results of LAI Assay in Rheumatoid Arthritis

    IV. Results of LAI Assay in Experimental Arthritis

    V. Significance of LAI Studies in Experimental Arthritis

    VI. Significance of LAI Studies in Rheumatoid Arthritis

    VII. Conclusions


    10 Cellular and Pharmacologic Aspects

    I. Introduction

    II. The Test in Arthritis

    III. Significance of the Nonadherence in the LAI

    IV. Cellular Aspects

    V. Sensitization of IgG Rheumatoid Factor

    VI. LAI Test and Pregnancy Serum

    VII. Characterization of Synovial Extract

    VIII. Activation of Polymorphonuclear Leukocytes

    IX. Leukocyte Adherence and Prostaglandins

    X. Leukocyte Adherence and ß Receptors

    XI. LAI Test with Synovial Fluid Leukocytes

    XII. Adherence and Immunomodulation

    XIII. Discussion


    III Leukocyte Adherence Inhibition in the Microplate

    11 Comparison of Microplate LAI with Other in Vitro Assays of Cell-Mediated Immunity: Assessment of Responses to Bacterial, Viral, and Tumor Antigens in Man and Experimental Animals

    I. Microplate LAI

    II. Microplate LAI Reactivity in Murine Tumor Systems

    III. Microplate LAI in the Assessment of Host Responses to Viral and Bacterial Antigens

    IV. Conclusions


    12 A Comparison of Tumor Immunity in Animal Models and Human Cancer

    I. Introduction

    II. Method

    III. Characterization of Antigen(s) Detected in the Microplate LAI Assay

    IV. Patterns of Sensitization Detected by the Microplate LAI Assay

    V. Serum Studies

    VI. Mechanism of Reactivity

    VII. Summary


    13 Cellular Requirements for the Leukocyte Adherence Inhibition Response to an Immunogenic Viral Induced Murine Tumor

    I. Introduction

    II. Methodology

    III. LAI Response of Mice to Regressing MSV-lnduced Tumors

    IV. LAI Response to Progressively Growing Tumors

    V. Mechanisms of the LAI Response to MSV-lnduced Tumors

    VI. Comparison with Mouse MIF Formation

    VII. Conclusions


    14 Leukocyte Adherence Inhibition Testing in Domestic Animals

    I. Introduction

    II. Methods Available for the Evaluation of Cellular Immunity in Domestic Animals

    III. LAI Testing

    IV. Experimental Use of the LAI Microassay

    V. Conclusions


    15 Leukocyte Adherence Inhibition: Measurement by Radioisotopic Labeled Cells

    I. Introduction

    II. Assay Using 111Indium-Labeled Cells



Product details

  • No. of pages: 398
  • Language: English
  • Copyright: © Academic Press 1982
  • Published: January 1, 1982
  • Imprint: Academic Press
  • eBook ISBN: 9780323152297

About the Editor

D Thomson

Ratings and Reviews

Write a review

There are currently no reviews for "Assessment of Immune status by the Leukocyte Adherence Inhibition Test"