This volume includes information on arachidonic acid metabolites and platelet activating factor. Assays for the quantitative measurement of prostaglandins, leukotrienes, and platelet-activating factor, including enzyme immunoassays and mass spectrophotometric techniques, are presented. Preparation and analysis of cytochrome P-450 metabolites of arachidonic acid are discussed. The isolation, purification, and assay of enzymes important in the biosynthesis and metabolism of these lipids are included.
Biochemists, pharmacologists, immunologists, hematologists, endocrinologists, physiologists, oncologists, and other biomedical researchers.
W.L. Smith, P. Borgeat, M. Hamberg, L.J. Roberts II, A.L. Willis, S. Yamamoto, P.W. Ramwell, J. Rokach, B. Samuelsson, E.J. Corey, and C.R. Pace-Asciak, Nomenclature.
I.A. Blair, Electron-Capture Negative-Ion Chemical Ionization Mass Spectrometry of Lipid Mediators.
P. Pradelles, J. Grassi, and J. Maclouf, Enzyme Immunoassays of Eicosanoids Using Acetylcholinesterase.
G. Ciabattoni, P. Patrignani, and C. Patrono, Radioimmunoassay of 11-Dehydrothromboxane B2.
F. Catella and G.A. FitzGerald, Measurement of Thromboxane Metabolites by Gas Chromatography-Mass Spectrometry.
D.F. Wendelborn, J.D. Morrow, and L.J. Roberts II, Quantification of Prostaglandin F2 Stable Isotope Dilution Mass Spectrometric Assay.
J.J. Vrbanac, J.W. Cox, T.D. Eller, and D.R. Knapp, Immunoaffinity Purification-Chromatographic Quantitative Analysis of Arachidonic Acid Metabolites.
J.-P. Lellouche, J.P. Beaucourt, and A. Vanhove, Preparation of Tetradeuterated Leukotriene A4 Methyl Ester: Methyl-[11,12,14,15-2H4]-(5S,6S)-Oxide-(7E,9E,11Z,14Z)-Eicosatetraenoate.
W.R. Mathews, Quantitative Gas Chromatography-Mass Spectrometry Analysis of Leukotriene B4.
P. Pradelles, C. Antoine, J.-P. Lellouche, and J. Maclouf, Enzyme Immunoassays for Leukotrienes C4 and E4 Using Acetylcholinesterase.
R.C. Murphy and A. Sala, Quantitation of Sulfidopeptide Leukotrienes in Biological Fluids by Gas Chromatography-Mass Spectrometry.
P. Borgeat, S. Picard, P. Vallerand, S. Bourgoin, A. Odeimat, P. Sirois, and P.E. Poubelle, Automated On-Line Extraction and Profiling of Lipoxygenase Products of Arachidonic Acid by High-Performance Liquid Chromatography.
- No. of pages:
- © Academic Press 1990
- 28th August 1990
- Academic Press
- eBook ISBN:
- Hardcover ISBN:
@from:Praise for the Volume @qu:"This volume of Methods in Enzymology brings up-to-date the new techniques in the measurement of eicosanoids, the application of molecular biological techniques to eicosanoids, the application of molecular biological techniques to eicosanoid research, and the developments in the study of the action and formation of eicosanoids in cell and tissue systems. The authors are to be commended for bringing together such a truly outstanding compendium, with top-rate articles by most of the leading scientists in the field. The book provides detailed information about individual aspects of eicosanoid research while, at the same time, encompassing the great breadth of the area. It is recommended for all individuals with an interest with eicosanoids and lipid mediators, from graduate students to eminent scientists. @source:--AMERICAN JOURNAL OF PHYSIOLOGY @qu:"Seemingly immortal series... the chapters are well written and the protocols...can be followed without further reference. This is only to be expected from a standard methods text." @source:--BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY @from:Praise for the Series @qu:"The Methods in Enzymology series represents the gold-standard." @source:--NEUROSCIENCE @qu:"Incomparably useful." @source:--ANALYTICAL BIOCHEMISTRY @qu:"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." @source:--BIO/TECHNOLOGY @qu:"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." @source:--CHEMISTRY IN INDUSTRY @qu:"The appearance of another volume in that excellent series, Methods in Enzymo